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Rna polymerase 2 antibody

Manufactured by Santa Cruz Biotechnology
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The RNA polymerase II antibody is a laboratory tool used to detect and study the RNA polymerase II enzyme, which is responsible for the transcription of protein-coding genes in eukaryotic cells. This antibody can be used in various techniques, such as immunoprecipitation, Western blotting, and immunofluorescence, to identify and analyze the presence and localization of RNA polymerase II within a sample.

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Lab products found in correlation

Hbc antibody, by Agilent Technologies (2 mentions) Brg1 antibody, by Abcam (2 mentions) Histone 3 antibody, by Active Motif (2 mentions) H3k27me3 antibody, by Abcam (2 mentions) Sall4 antibody, by Abcam (2 mentions) Stat3 antibody, by Santa Cruz Biotechnology (2 mentions) Oct4 antibody, by Abcam (2 mentions) Iscript cdna synthesis kit, by Bio-Rad (2 mentions)

Close spelling variants of this product

Anti-RNA Polymerase II antibody RNA polymerase II Polymerase II

4 protocols using rna polymerase 2 antibody

1

Chromatin Immunoprecipitation Protocol

Cited 1 time
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These analyses were carried out as previously described7 (link). The following antibodies were used in this study: SALL4 antibody (Abcam, Cambridge, MA); HBc antibody (Dako, Carpinteria, CA); RNA polymerase II antibody (Santa cruz biotechnology, Dallas, Texas); BRG1 antibody (Abcam, Cambridge, MA); STAT3 antibody (Santa Cruz biotechnology, Dallas, Texas); OCT4 antibody (Abcam, Cambridge, MA); H3K27me3 antibody (Abcam, Cambridge, MA); Histone 3 antibody (Active Motif, Carlsbad, CA). ChIP primer sequences listed in Supplementary Table 1.
Fan H., Cui Z., Zhang H., Kailasam Mani S.K., Diab A., Lefrancois L., Fares N., Merle P, & Andrisani O. (2016). DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection. Oncogene, 36(17), 2435-2445.
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2

RNA-Protein Interaction Protocol

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We followed the standard protocol described previously31 . RNA polymerase II antibody (Santa Cruz biotechnology, Dallas, Texas) was used for IP and iScript cDNA Synthesis Kit (Bio-Rad, Hercules, California) was employed for reverse transcription of RNA. RIP primer sequences are available in Supplementary Table 1.
Fan H., Cui Z., Zhang H., Kailasam Mani S.K., Diab A., Lefrancois L., Fares N., Merle P, & Andrisani O. (2016). DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection. Oncogene, 36(17), 2435-2445.
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3

Chromatin Immunoprecipitation Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
These analyses were carried out as previously described7 (link). The following antibodies were used in this study: SALL4 antibody (Abcam, Cambridge, MA); HBc antibody (Dako, Carpinteria, CA); RNA polymerase II antibody (Santa cruz biotechnology, Dallas, Texas); BRG1 antibody (Abcam, Cambridge, MA); STAT3 antibody (Santa Cruz biotechnology, Dallas, Texas); OCT4 antibody (Abcam, Cambridge, MA); H3K27me3 antibody (Abcam, Cambridge, MA); Histone 3 antibody (Active Motif, Carlsbad, CA). ChIP primer sequences listed in Supplementary Table 1.
Fan H., Cui Z., Zhang H., Kailasam Mani S.K., Diab A., Lefrancois L., Fares N., Merle P, & Andrisani O. (2016). DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection. Oncogene, 36(17), 2435-2445.
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4

RNA-Protein Interaction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
We followed the standard protocol described previously31 . RNA polymerase II antibody (Santa Cruz biotechnology, Dallas, Texas) was used for IP and iScript cDNA Synthesis Kit (Bio-Rad, Hercules, California) was employed for reverse transcription of RNA. RIP primer sequences are available in Supplementary Table 1.
Fan H., Cui Z., Zhang H., Kailasam Mani S.K., Diab A., Lefrancois L., Fares N., Merle P, & Andrisani O. (2016). DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection. Oncogene, 36(17), 2435-2445.
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