Isopropyl thio β d galacto pyranoside

The materials and sources used in this study are as follows: dithiothreitol (DTT), 17-β-estradiol (E2), ethylenediaminetetraacetic acid (EDTA), L-glutathione (reduced), 1-hydroxypyrene (1-HP), 4-hydroxytamoxifen (TAM), imidazole, isopropyl-thio-β-D-galactopyranoside (IPTG), Lysogeny broth (LB), lysozyme, pepstatin A, raloxifene (Ral), and sodium phosphate were the highest grade available from Sigma. Ampicillin, HEPES, KCl, KOH, MgCl₂, NaCl and phenylmethylsulfonyl fluoride (PMSF) were purchased from Fisher Scientific. Epigallocatechin gallate (EGCG) and epigallocatechin (EGC) were obtained from Santa Cruz Biotechnology, Inc. Anion exchange HPLC was performed using an Eprogen, AX100 (5μm) column. Glutathione- and nickel-chelating resins were obtained from GE Healthcare. Competent E. coli (BL21(DE3)) was purchased from Novagen. PAPS and PAP were synthesized in house as previously described (16 (link), 31 (link)) and were > 98% pure as assessed by anion-exchange high- performance liquid chromatography.

The materials and sources used in this study are as follows: dithiothreitol (DTT), ethylenediaminetetraacetic acid (EDTA), L-glutathione (reduced), 1-hydroxypyrene (1-HP), imidazole, isopropyl-thio-β-D-galacto-pyranoside (IPTG), LB media, lysozyme, pepstatin A, potassium phosphate, and 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid (TSP) sodium salt were the highest grade available from Sigma. Ampicillin, HEPES, KCl, KOH, MgCl₂ and phenylmethylsulfonyl fluoride (PMSF) were purchased from Fisher Scientific. Acetaminophen was obtained from CVS Pharmacy, liquid gel tablets, lot 1001402. Dehydroepiandrosterone (DHEA), the chemical, was purchased from TCI America, DHEA [1,2,6,7-³H(N)] was obtained from PerkinElmer, DHEA, the nutraceutical, was obtained from the General Nutrition Corporation, lot 57429B17. Mefenamic acid, the chemical, was obtained from Santa Cruz Biotechnology. Mefenamic acid, the drug, was obtained from Lupin Limited, Lot G700974. Deuterium Oxide (> 99%) was purchased from Acros Organics. Glutathione- and nickel-chelating resins were obtained from GE Healthcare. Competent E. coli (BL21(DE3)) was purchased from Novagen. PAPS was synthesized as previously described [17 (link),18 (link)] and was ≥99% pure as assessed by anion-exchange HPLC.