Glutathione reduced form

PrimeSTAR® MAX DNA Polymerase and In-Fusion® HD Cloning Kit were purchased from Takara Bio Inc (Shiga, Japan). LB broth (Miller) was purchased from Merck Millipore Corp (Billerica, MA, USA). Tryptone, extract yeast dried, tris (hydroxymethyl) aminomethane (Tris), acrylamide/bis mixed solution (29:1), and Rapid Stain CBB kit were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Prestained XL-Ladder was purchased from APRO Science Inc (Tokushima, Japan). Hydrochloric acid, sodium chloride, potassium chloride, disodium hydrogenphosphate 12-water, dipotassium hydrogenphosphate, potassium dihydrogen phosphate, glutathione (reduced form), Fe(III) chloride hexahydrate, and trichloroacetic acid were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). N,N,N',N'-tetramethylethylenediamine, ammonium persulfate, and hydroxylamine hydrochloride were purchased from Kishida Chemical Co.,Ltd (Osaka, Japan). Z-QG was purchased from Peptide Institute, Inc. (Osaka, Japan). L-glutamic acid γ-monohydroxamate was purchased from Sigma-Aldrich Co. LLC (St. Louis, MO). Ultrapure water supplied by Milli-Q® Reference Water Purification System (Merck Millipore Corp., Billerica, MA, USA) was used for buffer preparation.