Log In Sign up
The largest database of trusted experimental protocols

Z arg arg pna

Manufactured by Bachem
Visit Supplier
Sourced in Switzerland, United States

Z-Arg-Arg-pNA is a synthetic peptide substrate used in biochemical and enzymatic assays. It is composed of the amino acid sequence Z-arginine-arginine-p-nitroaniline. This substrate can be utilized to measure the activity of proteolytic enzymes that cleave the arginine-pNA bond, releasing the chromogenic p-nitroaniline group which can be detected spectrophotometrically.

Automatically generated - may contain errors

Lab products found in correlation

Z phe arg pna, by Bachem (1 mentions) Z arg arg pna, by Merck Group (1 mentions) Total nitric oxide assay kit, by Beyotime (1 mentions)

6 protocols using z arg arg pna

1

Proteinase Activity Measurement Using ZArg-Arg-pNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteinase activity was measured using the synthetic peptide ZArg-Arg-pNA (Bachem) as a substrate [129 (link)]. 20 μl of cell-free supernatants collected from the secreted aSMase activity assays were combined with 180 μl of PBS and 2 μl of the 10 mM stock substrate for 2 h at 37 °C, reading every 15 min. The release of p-nitroaniline was measured in a microplate reader (Multiskan Go Thermo Scientific) at 405 nm. One unit of activity is defined as the number of micromoles of substrate hydrolyzed per min.
Ramírez-Montiel F., Mendoza-Macías C., Andrade-Guillén S., Rangel-Serrano Á., Páramo-Pérez I., Rivera-Cuéllar P.E., España-Sánchez B.L., Luna-Bárcenas G., Anaya-Velázquez F., Franco B, & Padilla-Vaca F. (2019). Plasma membrane damage repair is mediated by an acid sphingomyelinase in Entamoeba histolytica. PLoS Pathogens, 15(8), e1008016.
+ Open protocol
+ Expand
2

Cysteine Peptidase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cysteine peptidase (CP) activity was measured using the synthetic peptide Z-Arg-Arg-pNA (Bachem, Bubendorf, Switzerland) as substrate [24 (link)]. One unit of enzymatic activity is defined as the amount of enzyme that catalyzes the generation of 1 mmol p-nitroaniline in 1 min. The assay was performed at least four times in duplicate. To determine the relative CP activity, the activity of the controls (B2p_pNC, B8np) was set to 100%. Significance was evaluated using the Mann-Whitney U test.
König C., Meyer M., Lender C., Nehls S., Wallaschkowski T., Holm T., Matthies T., Lercher D., Matthiesen J., Fehling H., Roeder T., Reindl S., Rosenthal M., Metwally N.G., Lotter H, & Bruchhaus I. (2020). An Alcohol Dehydrogenase 3 (ADH3) from Entamoeba histolytica Is Involved in the Detoxification of Toxic Aldehydes. Microorganisms, 8(10), 1608.
+ Open protocol
+ Expand
3

Measuring Cathepsin Protease Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
CP activity was measured using the synthetic peptide Z-Arg-Arg-pNA (Bachem, Bubendorf, Switzerland) as substrate [72 (link)]. One unit of enzymatic activity is defined as the amount of protein that catalyses the generation of 1 μmoL p-nitroaniline per min.
Meyer M., Fehling H., Matthiesen J., Lorenzen S., Schuldt K., Bernin H., Zaruba M., Lender C., Ernst T., Ittrich H., Roeder T., Tannich E., Lotter H, & Bruchhaus I. (2016). Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation. PLoS Pathogens, 12(8), e1005853.
+ Open protocol
+ Expand
4

Substrate Specificity Analysis of Proteases

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Substrate specificity was studied using the 0.25 mM chromogenic substrates Z-Phe-Arg-pNA (where Z = benzyloxycarbonyl), Z-Arg-Arg-pNA (both from Bachem, Switzerland), Glp-Phe-Gln-pNA, Glp-Phe-Leu-pNA, Glp-Phe-Ala-pNA, and Glp-Val-Ala-pNA, as described in Section 4.4. The substrates Glp-Phe-Leu-pNA, Glp-Val-Ala-pNA, Glp-Phe-Ala-pNA, Glp-Phe-Ala-AMC, and Glp-Phe-Gln-pNA were synthesized, as described in [41 (link),43 (link),47 ].
Dvoryakova E.A., Klimova M.A., Simonyan T.R., Dombrovsky I.A., Serebryakova M.V., Tereshchenkova V.F., Dunaevsky Y.E., Belozersky M.A., Filippova I.Y, & Elpidina E.N. (2022). Recombinant Cathepsin L of Tribolium castaneum and Its Potential in the Hydrolysis of Immunogenic Gliadin Peptides. International Journal of Molecular Sciences, 23(13), 7001.
+ Open protocol
+ Expand
5

Cysteine Protease Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
CPs activity was monitored by cleavage of the synthetic substrate benzyloxycarbonyl-l-arginyl-l-arginine-p-nitroanilide (z-Arg-Arg-pNA) (Bachem, Torrance, CA, USA) using a previously described protocol [27 (link)] except that DTT was not systematically added to the reaction buffer. Briefly, z-Arg-Arg-pNA was incubated for 0–10 min at 37 °C with E. histolytica lysate (40 µg) (prepared in phosphate buffer saline (PBS) nonidet P-40 (1%) (Sigma-Aldrich, Israel) in 990 µL CP buffer (0.1 M KH2PO4, 2 mM EDTA, pH 7.0). Cleavage of z-Arg-Arg-pNA substrate were detected at 405 nm in a Novaspec plus spectrophotometer (Sigma-Aldrich, Israel).
Sarid L., Zanditenas E., Ye J., Trebicz-Geffen M, & Ankri S. (2022). Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics. Antioxidants, 11(5), 814.
+ Open protocol
+ Expand
6

Measurement of CTSB, XOR, and NO

Check if the same lab product or an alternative is used in the 5 most similar protocols
CTSB activity of whole cell extracts was measured in 100 mM sodium acetate and 5 mM CaCl 2 (pH 5.5) containing 10 mM DTT. Substrates benzyloxycarbonyl-Arg-Arg-p-nitroaniline (Z-Arg-Arg-pNA) was purchased from Bachem (CA, USA). Cleavage of the substrate resulted in free pNA that was detected colorimetrically at 405 nm. XOR activity was measured by XOR Assay Kit (Jiancheng Biotech, Nanjing, China) according to the manufacturer's recommendations. NO levels were measured by Total Nitric Oxide Assay Kit (Nitrate/Nitrite Assay Kit, Beyotime) according to the manufacturer's recommendations. CTSB concentration in serum was measured by Human Cathepsin B ELISA kit (Cusabio, Wuhan, China) according to the manufacturer's recommendations.
Lin Z., Zhao S., Li X., Miao Z., Cao J., Chen Y., Shi Z., Zhang J., Wang D., Chen S., Wang L., Gu A., Chen F., Yang T., Sun K., Han Y., Xie L., Chen H, & Ji Y. (2023). Cathepsin B S-nitrosylation promotes ADAR1-mediated editing of its own mRNA transcript via an ADD1/MATR3 regulatory axis. Cell research, 33(7).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!