Fpquest software
FPQuest software is a data analysis tool designed for use with Bio-Rad's fluorescence polarization (FP) instruments. It provides basic functionality to analyze and manage FP data.
Lab products found in correlation
19 protocols using fpquest software
PCR Melting Profile Analysis
Genomic Diversity Analysis of E. coli
Genotyping and Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates
PCR Fingerprinting Protocol Validation
Pulsed-Field Gel Electrophoresis for Bacterial DNA Analysis
Chromosomal Comparison of V. parahaemolyticus Using REP-PCR
Microbial Diversity Analysis using MLVA
Molecular Typing of S. aureus Isolates
The PFGE macrorestriction profiles were analyzed using FPQuest software (Bio-Rad, Marnes-la-Coquette, France). Classification of individual restriction patterns for each genetic profile was conducted using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) and Dice’s coefficient (2.0%). PFGE results were presented as a dendrogram.
Molecular Profiling of Salmonella Isolates
S. Infantis isolates (n = 26), S. Manhattan isolates (n = 7), and one O-untypeable:r:1,5 isolate that were shown to carry resistance genes (blaCMY, blaTEM, blaCTX-M, and/or blaSHV) were further examined using pulsed-field gel electrophoresis (PFGE) profiling and large-plasmid profile (LPP) analysis. To compare isolates with and without resistance genes, S. Infantis isolates (n = 18) and S. Manhattan isolates (n = 18) showing susceptibility to 11 antimicrobials were also examined. Two, three, five, four, and four susceptible S. Infantis isolates were collected in 2006, 2007, 2008, 2009, and 2010, respectively. Of the 18 susceptible S. Manhattan isolates, two, one, two, three, two, two, three, and three were collected in 2003, 2004, 2005, 2006, 2007, 2008, 2009, and 2010, respectively. PFGE using BlnI restriction digestion was conducted as previously described [30 (link)]. Similarity and cluster analyses were performed using the Dice coefficients of similarity and an unweighted pair group method with average linkage, respectively, using FPQuest Software (Bio-Rad Laboratories, Hercules, CA). For LPP analysis, total DNA was treated with 2 U/ml of S1 nuclease (incubated at 37°C for 45 min), followed by PFGE separation [30 (link)].
Pulsed-Field Gel Electrophoresis for Bacterial Strain Typing
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