The ovaries used in the histological analysis were fixed in Bouin’s solution for 24 h. They were then dehydrated with ethanol and clarified with xylene prior to being embedded in paraffin, followed by sectioning to a 5–7 μm thickness. The sections were stained with Hematoxylin Eosion (HE). Images were captured using a Nikon E80 microscope with a DS-Ri2 Imaging System (Nikon, Tokyo, Japan).
Ds ri2 imaging system
Manufactured by Nikon
Sourced in Japan
The DS-Ri2 is a digital imaging system designed for microscopy applications. It features a high-resolution CMOS sensor and advanced image capture capabilities. The system is capable of capturing detailed images for various research and analysis purposes.
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Lab products found in correlation
5 protocols using ds ri2 imaging system
1
Histological Analysis of Ovarian Tissue
2
Immunohistochemical Analysis of RNF180 Expression in Lung Cancer
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The tumorous or normal lung tissue sections (4–7 μm in thickness) were incubated with RNF180 antibodies (ab127548, Abcam, UK) overnight at 4°C, followed by incubation with secondary antibodies (D-3004; Long Island Biotech, China) for 30 min at 25°C. For IHC staining, 3,3′-diaminobenzidine (DAB) substrate (Long Island Biotech, USA) and hematoxylin 714094 (BASO Diagnostic Inc., China) were used. The ECLIPSE Ni-E/Ni-U microscope (Nikon, Japan) with DS-Ri2 imaging system (Nikon) was used to visualize RNF180-positive cells. The tumor cells with a positive stain of >25% were considered as having high RNF180 expression according to the methods as previous reports mentioned [24 (link), 25 (link)], whereas those with <25% were considered as having low RNF180 expression.
3
Histological Evaluation of Testis Tissue
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A testis lobe from each fish was fixed in Bouin’s solution for 24 h, dehydrated in a series of ethanol, clarified in xylene and embedded in paraffin. The tissues were embedded in paraffin and sectioned with a microtome (Leica RM 2155) at 3 μm thicknesses. All tissue sections were stained with HE. Images were captured by a Nikon Ni-E microscope equipped with a Nikon DS-Ri2 imaging system. All sections from each male fish were examined in a random fashion. Each cell type (spermatogonia, spermatocyte, spermatid, and spermatozoa) at each section of the border and inside of the cyst was scored and five random images per fish were analysed.
4
IHC Analysis of TMEM14A and c-Myc in OV
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The tumorous or normal OV tissue sections (4-7 µm in thickness) were incubated with TMEM14A (1:350, cat. no. PA5-112745; Thermo Fisher Scientific, Inc.) or c-MYC (1:1,000; cat. no. D84C12; Cell Signaling Technology, Inc.) antibodies overnight at 4˚C, followed by incubation with HRP-labeled secondary antibodies (cat. no. D-3004; Long Island Biotech) for 30 min at 25˚C. For IHC staining, 3,3'-diaminobenzidine (DAB) substrate (Shanghai Long Island Biotechnology Co., Ltd.) and hematoxylin 714094 (BASO Diagnostic Inc.) were used. The ECLIPSE Ni-E/Ni-U microscope with DS-Ri2 imaging system (Nikon Corporation) was used to visualize TMEM14A or c-MYC-positive cells. The results were read by two independent pathologists according to a previous report (30 (link)). Stain intensity and the percentage of positive cells were scored as follows: i) for stain intensity, negative, score 0; light brown, score 1; brown, score 2; deep brown, score 3; ii) for percentage of positive cells, scored each criterion on a scale of 0 to 3, ≤5%, scored 0; 6-25% scored 1, 26-50% scored 2 and >50% scored 3. The final IHC-score (0-9) were calculated by multiplying the scores of the percentage of positive cells by the stained intensity.
5
Immunohistochemical Staining of RNF180 in Lung Tissue
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The tumorous or normal lung tissue sections (~ 4-7 μm thick) were incubated with RNF180 antibody (ab127548, Abcam) overnight at 4°C and then with secondary antibodies (D-3004, Long Island Biotech, China) for 30 min at 25°C. DAB (3,3'-diaminobenzidine) substrate (Long Island Biotech, USA) and hematoxylin 714094 (BASO Diagnostic Inc., China) were applied for IHC staining. The Eclipse Ni-E/Ni-U microscope (NIKON, Japan) with DS-Ri2 imaging system (NIKON, Japan) was used to visualize and count RNF180-postive cells.
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