Disodium hydrogen phosphate

Curcumin extract (90%) was purchased from Sisco Research Laboratories (SRL) Pvt. Ltd., (Mumbai, Maharashtra, India). High Capacity cDNA kit, phosphate buffer saline (PBS), formaldehyde, 5,5-dithiobis (2-nitrobenzoic acid) (DTNB), trichloroacetic acid, thiobarbituric acid (TBA), butanol, nitroblue tetrazolium (NBT), disodium hydrogen phosphate, sodium citrate, and hydrogen peroxide (H₂O₂) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Mouse brain-derived neurotrophic factor (BDNF) ELISA kit and mouse CREB ELISA kit were purchased from Ray Biotech (Georgia, GA, USA). Mouse Synapsin ELISA kit and mouse DCX ELISA kit were purchased from Gentaur (Kampenhout, Belgium). RNA Isolation Reagents and TRIzol were purchased from Sigma Aldrich (St. Louis, MO, USA). SYBR Green was purchased from Applied Biosystems, MA, USA. NanoDrop (NanoDrop Technologies, Wilmington, DE, USA), Polytron tissue homogenizer (Thomas Scientific, Swedesboro, NJ, USA), ELISA reader (Trans Asia Pvt. Ltd., Mumbai, Maharashtra, India), and UV spectrometer (Perkin Elmer, Waltham, MA, USA) were used during the conduction of experimental procedures.

Individual PAHs (naphthalene, acenaphthene, anthracene, pyrene, chrysene, fluorine and acenaphthylene) were purchased from AccuStandard (New Haven, CT) as 1000 µg/µL solutions in methanol. Ethanol (99.8% HPLC grade), sodium chloride (CAS No. 7647-14-5), sodium dihydrogen phosphate monohydrate (CAS No. 10049-21-5), disodium hydrogen phosphate (CAS No. 7558-79-4), ammonium sulfate (CAS No. 7783-20-2) and Lysogeny broth (LB) (CAS No. 69-52-3) were purchased from Thermo Fisher Scientific. Antifoam 204 (A6426), IPTG (CAS No. 367-93-1), glucose (CAS No. 50-99-7), guanidine hydrochloride (CAS No. 50-01-1), and imidazole (CAS No. 288-32-4) were obtained from Sigma-Aldrich.

Gold (III) chloride trihydrate was purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium hyaluronate (50 kDa) was acquired from Lifecore Biomedical Inc. (Chaska, MN, USA). Potassium bromide (KBr), sodium hydroxide (NaOH), and paraformaldehyde were procured from Sigma Aldrich (St. Louis, MO, USA). Sodium chloride and potassium chloride were obtained from Sigma Aldrich (Bangalore, India). Disodium hydrogen phosphate was procured from Thermo Fischer Scientific (Mumbai, India). Potassium dihydrogen phosphate was acquired from Merck (Mumbai, India). Leibovitz (L-15) medium, Fetal bovine serum (FBS), trypsin, and penicillin-streptomycin were obtained from Gibco (Waltham, MA, USA). 2′,7′-Dichlorofluorescein diacetate (DCFDA) was purchased from Abcam (Cambridge, UK). A dead cell apoptosis kit with annexin V was procured from Invitrogen (Waltham, MA, USA). Propidium iodide was purchased from Sigma Aldrich (St. Louis, MO, USA). BODIPY C11 probe was purchased from Invitrogen (Waltham, MA, USA). Human recombinant CD44 protein was purchased from Invitrogen (Mumbai, India). Mouse monoclonal anti-CD44 antibody and goat anti-mouse IgG H&L (Alexa Flour 647) were purchased from Abcam (Cambridge, UK).