Standard antimicrobial discs
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Standard antimicrobial discs are a type of laboratory equipment used for antimicrobial susceptibility testing. These discs are impregnated with a known concentration of an antimicrobial agent and are placed on a culture medium inoculated with a bacterial sample. The size of the resulting zone of inhibition around the disc is used to determine the susceptibility of the bacteria to the tested antimicrobial agent.
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Lab products found in correlation
2 protocols using standard antimicrobial discs
1
Antimicrobial Activity of Biosurfactant Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
(i) Agar well assay. RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs were screened for activity against all bacterial isolates using an agar well diffusion assay (73 (link)). Bacterial suspensions in sterile saline corresponding to an optical density of a 0.5 McFarland standard (1.5 × 108 CFU/mL) were grown in Mueller-Hinton (MH) agar (Oxoid Ltd., England). Wells (8 mm) were cut into each inoculated agar plate, and a 100-μL aliquot of each NP solution (10 μg/mL concentration) was pipetted into each well. NPs were replaced with sterile water as a negative control for bacterial growth and standard antimicrobial discs (Oxoid, Cambridge, UK). Imipenem (IPM; 10 μg) and ciprofloxacin (CIP; 5 μg) were used as positive control for bactericidal action for E. coli , while vancomycin (VA; 30 μg), ciprofloxacin (CIP; 5 μg), and oxacillin (OX; 1 μg) were used for S. aureus . The plates were incubated at 37°C for 24 h. After incubation, zones of growth inhibition were measured to the nearest millimeter to determine the antimicrobial potency of the screened antimicrobial substances (74 (link)). The results are expressed as mean ± standard deviation (75 (link)).
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2
Antimicrobial Activity of Biosurfactant Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
(i) Agar well assay. RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs were screened for activity against all bacterial isolates using an agar well diffusion assay (73 (link)). Bacterial suspensions in sterile saline corresponding to an optical density of a 0.5 McFarland standard (1.5 × 108 CFU/mL) were grown in Mueller-Hinton (MH) agar (Oxoid Ltd., England). Wells (8 mm) were cut into each inoculated agar plate, and a 100-μL aliquot of each NP solution (10 μg/mL concentration) was pipetted into each well. NPs were replaced with sterile water as a negative control for bacterial growth and standard antimicrobial discs (Oxoid, Cambridge, UK). Imipenem (IPM; 10 μg) and ciprofloxacin (CIP; 5 μg) were used as positive control for bactericidal action for E. coli , while vancomycin (VA; 30 μg), ciprofloxacin (CIP; 5 μg), and oxacillin (OX; 1 μg) were used for S. aureus . The plates were incubated at 37°C for 24 h. After incubation, zones of growth inhibition were measured to the nearest millimeter to determine the antimicrobial potency of the screened antimicrobial substances (74 (link)). The results are expressed as mean ± standard deviation (75 (link)).
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