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Ab183727

Manufactured by Abcam
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Ab183727 is a laboratory reagent produced by Abcam. It is a monoclonal antibody that can be used for various research applications, but no further details about its intended use or functionality are provided in order to maintain an unbiased and factual approach.

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Lab products found in correlation

Ab77735, by Abcam (3 mentions) Ab155091, by Abcam (3 mentions) Ab4389, by Abcam (2 mentions) Ab3328, by Abcam (2 mentions) Ab3329, by Abcam (2 mentions) Sc 47197 n 17, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated 3f10, by Merck Group (2 mentions) Infrared imaging technology, by LI COR (2 mentions) Ab90867, by Abcam (1 mentions) Ab7291, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Mini protean transfer system, by Bio-Rad (1 mentions)

3 protocols using ab183727

1

Immunoblotting Analysis of Immune Proteins

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Cells were washed with PBS, lysed in sample buffer, boiled for 3 min, resolved by SDS-PAGE and analyzed by immunoblotting using primary antibodies specific for human MX2 (sc-47197 (N-17), Santa Cruz Biotechnology), human TRIM5α (ab4389, Abcam), human PSMB8 (ab3329, Abcam), human PSMB9 (ab3328, Abcam), human PSMB10 (ab77735, Abcam), human PA28A (ab155091, Abcam), human PA28B (ab183727, Abcam), FLAG (HRP-conjugated M2, Sigma), HA (HRP-conjugated 3F10, Sigma) or human α-tubulin (DM1A, Sigma), and detected using either horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (ECL+ western blotting substrate, Pierce) or IRDye®-800CW-labelled secondary antibodies and the LI-COR infrared imaging technology (LI-COR UK LTD).
Jimenez-Guardeño J.M., Apolonia L., Betancor G, & Malim M.H. (2019). Immunoproteasome activation enables human TRIM5α restriction of HIV-1. Nature microbiology, 4(6), 933-940.
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2

Immunoblotting Analysis of Immune Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with PBS, lysed in sample buffer, boiled for 3 min, resolved by SDS-PAGE and analyzed by immunoblotting using primary antibodies specific for human MX2 (sc-47197 (N-17), Santa Cruz Biotechnology), human TRIM5α (ab4389, Abcam), human PSMB8 (ab3329, Abcam), human PSMB9 (ab3328, Abcam), human PSMB10 (ab77735, Abcam), human PA28A (ab155091, Abcam), human PA28B (ab183727, Abcam), FLAG (HRP-conjugated M2, Sigma), HA (HRP-conjugated 3F10, Sigma) or human α-tubulin (DM1A, Sigma), and detected using either horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (ECL+ western blotting substrate, Pierce) or IRDye®-800CW-labelled secondary antibodies and the LI-COR infrared imaging technology (LI-COR UK LTD).
Jimenez-Guardeño J.M., Apolonia L., Betancor G, & Malim M.H. (2019). Immunoproteasome activation enables human TRIM5α restriction of HIV-1. Nature microbiology, 4(6), 933-940.
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3

Proteasome Subunit Quantification by Western Blot

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Proteins were transferred to PVDF membranes using a Bio-Rad mini-protean transfer system containing a buffer of 25 mM Tris base, 192 mM glycine, 0.1% SDS. Proteins were transferred for 6h at 40V at 4°C. Membranes with transferred proteins were blocked with a 1:1 mixture of Li-COR blocking buffer and PBS. Membranes were then incubated in blocking solution for 1 h and primary Abs (1:1000) applied for 16 h at 4°C. Membranes were washed and incubated with secondary Abs (IR Dye® 800CW goat anti-mouse IgG (H+L) or IR Dye 680CW donkey anti-rabbit IgG (H+L), and imaged using an Odyssey Li-Cor platform. Antibodies used were rabbit anti-PSMB5 polyclonal (Abcam, ab90867), rabbit monoclonal to anti-PSME1 (EPR10967, Abcam, ab155091), rabbit monoclonal to anti-PSME2 (EPR14931, Abcam, ab183727), rabbit anti-LMP2 polyclonal (PSMB9, Abcam, ab42987), rabbit anti-PSMB10 polyclonal (Abcam, ab77735), rabbit anti-LMP7 monoclonal (EPR14482, PSMB8, Abcam, ab180606), rabbit anti-PSMA6 polyclonal (Cell Signaling 2459), rabbit anti-PSMB1 polyclonal (Abcam, ab196623), rabbit anti-PSMB2 polyclonal (Abcam, ab140426), mouse anti-Rpn5 monoclonal (PSMD12, clone H3, Santa Cruz Biotechnology, sc-398279), mouse anti-α-tubulin monoclonal (DM1a, Abcam, ab7291), and rabbit anti-histone 3 polyclonal (Abcam, ab1791).
Woodle E.S., Tremblay S., Brailey P., Girnita A., Alloway R.R., Aronow B., Dasgupta N., Ebstein F., Kloetzel P.M., Lee M.J., Kim K.B., Singh H, & Driscoll J.J. (2019). Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 20(2), 399-410.
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