Transposons encoding the constitutively activated forms of TAZ (pT3/EF5a TAZS89A) was a kind gift from Dr. Xin Chen at the University of California, San Francisco. The plasmids pT2/EGFP and pPGK-SB13 were described previously [27 (link)]. Open reading frames (ORFs) encoding the activated forms of BRAF (BRAFV600E) and PIK3CA (PI3KE545K) were polymerase chain reaction (PCR)-amplified from pBabe-B-RAF-V600E (#17,544; Addgene, Watertown, MA, USA) and pBabe-puro-HA-PIK3CA-E545K plasmids (#12,525; Addgene), respectively. These amplified PCR products were cloned into pT2/BH transposon vectors (#26,556; Addgene) to generate the plasmids, pT2/BRAFV600E, and pT2/PI3KE545K, respectively.
Pt2 bh transposon vectors
Manufactured by Addgene
The PT2/BH transposon vectors are genetic tools used for the insertion and expression of genes. These vectors contain a transposon sequence that can be used to integrate genetic material into the host genome. The core function of these vectors is to facilitate the stable integration and expression of genes of interest in various cell types.
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Lab products found in correlation
2 protocols using pt2 bh transposon vectors
1
Generation of Activated TAZ, BRAF, and PI3K Constructs
2
Activated YAP, TAZ, BRAF, and PIK3CA Transposon Vectors
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Transposons encoding the constitutively activated forms of YAP (pT3/EF5a YAPS127A) and TAZ (pT3/EF5a TAZS89A) were kind gifts from Dr. Xin Chen at the University of California, San Francisco. The plasmids pT2/EGFP and pPGK-SB13 were described previously (Moon et al. 2017 ). Open reading frames (ORFs) encoding the activated forms of BRAF (BRAF V600E ) and PIK3CA (PI3K E545K ) were polymerase chain reaction (PCR)-ampli ed from pBabe-B-RAF-V600E (#17544; Addgene, Watertown, MA, USA) and pBabe-puro-HA-PIK3CA-E545K plasmids (#12525; Addgene), respectively. These ampli ed PCR products were cloned into pT2/BH transposon vectors (#26556; Addgene) to generate the plasmids, pT2/ BRAF V600E , and pT2/ PI3K E545K , respectively.
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