Anti cd182 cxcr2

Manufactured by BioLegend

Sourced in United States

Anti-CD182 (CXCR2) is a laboratory reagent used to identify and study cells expressing the CXCR2 chemokine receptor. It can be used in flow cytometry and other immunoassays.

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2 protocols using anti cd182 cxcr2

Multiparameter Immune Cell Profiling

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Anti-mouse ABs: anti-CD3e (clone: 145-2C11), anti-CD34 (clone: HM34), anti-CD45R (clone: RA3-6B2), anti-CD117 (c-KIT, clone: 2B8), anti-CD182 (CXCR2, clone: SA044G4), anti-CD184 (CXCR4, clone: L276F12), anti-Ly-6A/E (Sca-1, clone: D7), anti-Ki-67 (clone: 11F6), anti-Ly-6G (clone: 1A8), anti-NK-1.1 (clone: PK1), anti-mouse/human-CD11b (clone: M1/70) all from BioLegend (San Diego, CA, USA), and anti-CD16/32 (clone: 93) and anti-CD62L (clone: MEL-14) (both from eBioscience, Thermo Fisher Scientific, Waltham, MA, USA). Anti-human ABs: anti-CD66b (clone: 80H3, Beckman Coulter, Brea, CA, USA), and anti-CD71 (clone: M-A712, BD Biosciences, Franklin Lakes, NJ, USA). Viability dye eFluor780 and eFluor506 (eBioscience, Thermo Fisher Scientific, Waltham, MA, USA) or DAPI (BioLegend, San Diego, CA, USA) were used to determine viable cells.

Siakaeva E., Pylaeva E., Spyra I., Bordbari S., Höing B., Kürten C., Lang S, & Jablonska J. (2019). Neutrophil Maturation and Survival Is Controlled by IFN-Dependent Regulation of NAMPT Signaling. International Journal of Molecular Sciences, 20(22), 5584.

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Comprehensive Immune Cell Profiling Protocol

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Anti-CD45 antibody was obtained from BD. FVS was obtained from eBioscience. TUNEL was obtained from Beyotime. Anti-Ly6g (1A8), anti-CD11b, anti-CD182 (CXCR2) and anti-CD192 (CCR2) antibodies were obtained from BioLegend. PECAM-1 (390) was obtained from Thermo Fisher Scientific. DNase I was obtained from Solarbio. Diphtheria toxin and fluorescein isothiocyanate (FITC)-albumin were obtained from Sigma–Aldrich. Acetylcysteine (L-NAC) was obtained from Absin.

Xiang Y., Dai J., Li Y., You Z., Zhang J., Huang X., Nie S., Chen Y., Xu L., Liu F., Jiang J, & Xu J. (2022). ROS-activated CXCR2+ neutrophils recruited by CXCL1 delay denervated skeletal muscle atrophy and undergo P53-mediated apoptosis. Experimental & Molecular Medicine, 54(7), 1011-1023.

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