Materials were purchased from standard sources: luteolin from Sigma (St Louis, MO, USA); MTT from Sigma; DMEM and FBS from Gibco BRL (Waltham, MA, USA); methanol and acetic acid (HPLC grade) from Fisher Scientific (Waltham, MA, USA); and dimethyl sulfoxide (DMSO) and acetone from KeLong Chemicals (Chengdu, China). Antibodies used include rat anti-mouse CD31 polyclonal antibody (BD Pharmingen™, Franklin Lake, NJ, USA), rabbit anti-mouse PCNA antibody (Abcam, Cambridge, UK), and rhodamine-conjugated secondary antibody (Abcam). NH2-PEG-PCL was purchased from ruixi Biological Technology Company (Xian, China).
C57 mice (6–8 weeks) and Sprague Dawley (SD) rats were purchased from the Animal Center Laboratory of Beijing HFK Bioscience Co Ltd (Beijing, People’s Republic of China). All mice were housed in a controlled temperature of 20–22°C, with a relative humidity of 50–60% and 12 hr light-dark cycles. The animals were provided with standard laboratory chow and tap water ad libitum. All animal procedures were performed following the protocol approved by the Institutional Animal Care and Treatment Committee of Sichuan University (Chengdu, People’s Republic of China) and treated humanely throughout the experimental period.
C57 mice (6–8 weeks) and Sprague Dawley (SD) rats were purchased from the Animal Center Laboratory of Beijing HFK Bioscience Co Ltd (Beijing, People’s Republic of China). All mice were housed in a controlled temperature of 20–22°C, with a relative humidity of 50–60% and 12 hr light-dark cycles. The animals were provided with standard laboratory chow and tap water ad libitum. All animal procedures were performed following the protocol approved by the Institutional Animal Care and Treatment Committee of Sichuan University (Chengdu, People’s Republic of China) and treated humanely throughout the experimental period.