Tb green premix 2

Total RNA was extracted using RNAiso Plus (9109, Takara, Japan) according to the manufacturer’s protocol. Sketch™ RT Master Mix (RR036A, Takara, Japan) was used to synthesize complementary DNA by reverse transcription of RNA into DNA. Quantitative real-time PCR (qRT-PCR) experiments were performed using TB-Green™ Premix™ II (RR820A, Takara, Japan). Primers were designed and synthesized by Servicebio. GAPDH was used as an endogenous reference gene. Relative gene expression was determined using the 2^−ΔΔCT method. The sequences of the employed PCR primers are shown below.

GAPDH-F 5′-CCTCGTCCCGTAGACAAAATG-3′

GAPDH-R 5′-TGAGGTCAATGAAGGGGTCGT-3′

HDAC1-F 5′-CACAAAGCCAATGCTGAGGAG-3′

HDAC1-R 5′-CGATGTCCGTCTGCTGCTTAT-3′

KPNA2-F 5′-AGAACCTTTGATGAACCTCCTGA-3′

KPNA2-R 5′-TTTTATCCAAGCCTCCACACTCT-3′

CHD1L-F 5′-GAAGACCTGAGTTTGGGTGATGT-3′

CHD1L-R 5′-CGCTTGCTTTCTTTTTCTTTGC-3′

PPP2R5B-F 5′-GCGGTATTTGGGACCCTCTA-3′

PPP2R5B-R 5′-TTCTGCTGCTCCTGTTGTTTTT-3′

RAD54B-F 5′-CAGACGAGAATCACCAGCGG-3′

RAD54B-R 5′-CCTAAGCCCATTTCATCAGCAA-3′

RUVBL1-F 5′-AGAGCACCACGAAGACGCA-3′

RUVBL1-R 5′-GCAATAGCAAGGGCCAAGG-3′

POLR2L-F 5′-CAAATGGGAAGCCTACCTGG-3′

POLR2L-R 5′-CAGCTTCTCAATCAGGTCCACG-3′

MUTYH-F 5′-ATCGCCTTTGACCAGGTAACC-3′

MUTYH-R 5′-ATGGCAGCTTGATTGAAGTCCC-3′

SPP1-F 5′-TACAGCCTGCACCCAGATCCTAT-3′

SPP1-R 5′-GCTTTCATTGGAATTGCTTGGA-3′

NEIL3-F 5′-GGCTGCTCCAATGAATGCTAA-3′

NEIL3-R 5′-CTCCCGTGGGTTAATCAAGATG-3′

Total RNA was extracted using RNAiso Plus (9109, Takara, Japan) according to the manufacturer’s protocol. Sketch™ RT Master Mix (RR036A, Takara, Japan) was used to synthesize complementary DNA via the reverse transcription of RNA into DNA. Quantitative real-time PCR (qRT–PCR) experiments were performed using TB-Green™ Premix™ II (RR820A, Takara, Japan). GAPDH was used as an endogenous reference gene. Relative gene expression was determined using the 2^−ΔΔCT method. The following is a list of the PCR primer sequences that were used to analyze the skin tissues.
GAPDH-F 5′-GGA​AGC​TTG​TCA​TCA​ATG​GAA​ATC-3′
GAPDH-R 5′-TGA​TGA​CCC​TTT​TGG​CTC​CC-3′
FSP1-F 5′- CTC​CGT​GGA​GAC​AGG​GTT​CG -3′
FSP1-R 5′- GGT​TCT​TCA​GGT​CTA​TCC​CCA​CTA -3′
CoQ-F 5′-CTC​ATG​CGG​TTG​GAC​AAG​C-3′
CoQ-R 5′-CCT​GCT​CCA​CGC​ATC​AGA​ATA -3′
GPX4-F 5′-CCG​CTG​TGG​AAG​TGG​ATG​AAG -3′
GPX4-R 5′-CTT​GTC​GAT​GAG​GAA​CTT​GGT​GAA -3′
The following primer sequences were used to verify the establishment of overexpressing cells.
Actin-F 5′- TGG​CAC​CCA​GCA​CAA​TGA​A -3′
Actin-R 5′- CTA​AGT​CAT​AGT​CCG​CCT​AGA​AGC​A -3′
FSP1-F 5′- CAA​GAT​CAA​CAG​CTC​CGC​CTA​C -3′
FSP1-R 5′- AGG​TGC​TCG​TTC​ACT​CTC​AGA -3′

Total RNA was extracted using RNAiso Plus (9109, Takara, Japan) according to the manufacturer’s protocol. Sketch™ RT Master Mix (RR036A, Takara, Japan) was used to synthesize complementary DNA by reverse transcription of RNA into DNA. Quantitative real-time PCR (qRT–PCR) experiments were performed using TB-Green™ Premix™ II (RR820A, Takara, Japan). Primers were designed and synthesized by Takara. Actin was used as an endogenous reference gene. Relative gene expression was determined using the 2^−ΔΔCT method. The sequences of the employed PCR primers are shown below.
Actin-F 5′-AAATGGTGAGGGTCGGTGAAC-3′
Actin-R 5′-CAAATCCTCTTTGCCACTG-3′
ALDH3B1-F 5′- GAACTACCCCGTGAACCTGAC -3′
ALDH3B1-R 5′- ACCTTCTCCGTGCCCTTACTA -3′
NRF2-F 5′-TAGATGACCATGAGTCGCTTGC -3′
NRF2-R 5′-GCCAAACTTGCTCCATGTCC -3′