Exebacase MICs and minimum bactericidal concentrations (MBCs) were determined by the BMD method using CAMHB-HSD media (11 (link), 13 , 16 , 17 ), comprising BBL Mueller-Hinton II (cation-adjusted) broth (catalog no. 212322, lot 5257869; Becton Dickinson, Franklin Lakes, NJ, USA) with 25% (vol/vol) horse serum (catalog no. H1270, lot no. 21C414; Sigma-Aldrich, St. Louis, MO) and 0.5 mM dl -dithiothreitol (final concentration), pH 7.2–7.4 (catalog no. 646563, lot no. MKCMO454; Sigma-Aldrich). MIC endpoints were read as described in CLSI document M100-ed33 (12 ). Antibiotic MICs and MBCs were determined as described in CLSI documents M07-A11 (16 ) and M26-A (17 ), respectively, and in CAMHB-HSD.
Bbl mueller hinton 2 cation adjusted broth
Manufactured by BD
Sourced in United States
BBL Mueller-Hinton II (cation-adjusted) broth is a microbiological culture medium used for antimicrobial susceptibility testing. It is formulated to meet the requirements specified in standard testing methods.
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Lab products found in correlation
3 protocols using bbl mueller hinton 2 cation adjusted broth
1
Exebacase Antimicrobial Susceptibility Testing
2
Antibiotic Susceptibility Testing Protocol
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Exebacase MICs were determined by broth microdilution using CAMHB-HSD medium (16 (link), 18 , 20 (link)), comprised of BBL Mueller-Hinton II (cation-adjusted) broth (catalog no. 212322, lot 1242967; Becton, Dickinson, Franklin Lakes, NJ) with horse serum (25% [vol/vol]) (catalog no. H1270, lot no. 21C414; Sigma-Aldrich, St. Louis, MO) and 0.5 mM dl -dithiothreitol (pH 7.2 to 7.4) (Sigma-Aldrich). As described in CLSI standard M07-A11 (20 (link)), daptomycin MICs were determined in CAMHB supplemented to 50 μg/mL with CaCl2, oxacillin MICs were determined in CAMHB with 2% NaCl, and vancomycin MICs were determined in CAMHB. In addition, oxacillin, daptomycin, and vancomycin MICs were generated against each strain using CAMHB-HSD. MIC panels were incubated for 18 to 20 h at 37°C.
3
Exebacase Antimicrobial Susceptibility Testing
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Exebacase MICs and minimum bactericidal concentrations (MBCs) were determined by the BMD method using CAMHB-HSD media (11 (link), 13 , 16 , 17 ), comprising BBL Mueller-Hinton II (cation-adjusted) broth (catalog no. 212322, lot 5257869; Becton Dickinson, Franklin Lakes, NJ, USA) with 25% (vol/vol) horse serum (catalog no. H1270, lot no. 21C414; Sigma-Aldrich, St. Louis, MO) and 0.5 mM dl -dithiothreitol (final concentration), pH 7.2–7.4 (catalog no. 646563, lot no. MKCMO454; Sigma-Aldrich). MIC endpoints were read as described in CLSI document M100-ed33 (12 ). Antibiotic MICs and MBCs were determined as described in CLSI documents M07-A11 (16 ) and M26-A (17 ), respectively, and in CAMHB-HSD.
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