Sybr green realtime pcr kit
The SYBR Green Realtime PCR Kit is a laboratory equipment product designed for real-time polymerase chain reaction (PCR) analysis. It contains SYBR Green I dye, which binds to double-stranded DNA, allowing for the detection and quantification of DNA sequences during the PCR process.
Lab products found in correlation
7 protocols using sybr green realtime pcr kit
Expression Analysis of Cerebrovascular Markers
Quantitative Analysis of MMP-9 Expression
Quantitative Real-Time PCR Protocol
Quantitative Real-Time PCR Analysis
Gene Expression Analysis by RT-qPCR
The sequences of primers used in RT-qPCR
Primers | Sequences (5′–3′) |
---|---|
DARS-AS1-F | CATCGGGACACGGAACTGG |
DARS-AS1-R | TGCAAAGAACTGCAGAAGACAC |
miR-628-5p-F | GCCGAGATGCTGACATATTTAC |
miR-628-5p-R | CTCAACTGGTGTCGTGGA |
MGEA5-F | TGTGGCCAAAAGCATGATGG |
MGEA5-R | GTACAAGAAAGTTGGGCACAGG |
CACNB2-F | GAAGGCAATCATAGGCGAGC |
CACNB2-R | GACCCTGCAGTACTCTGCTT |
JAG1-F | CAAACACCAGCAGAAAGCCC |
JAG1-R | TAAGTCAGCAACGGCCTCAG |
ARRDC3-F | GAGACCACTGAGACGAGCG |
ARRDC3-R | TTCACCTTTCCCAGCACCAT |
VCAN-F | CTCGCAGAAACTGCATCACC |
VCAN-R | CTGAGGTTGGACTGTGGCTT |
GAPDH-F | CTCTGCTCCTCCTGTTCGAC |
GAPDH-R | TTCCCGTTCTCAGCCTTGAC |
U6-F | TCATCAGAAACAGTGGAGGT |
U6-R | CATCCTTACACAGGAGCCAT |
Quantitative Analysis of lncRNA and miRNA Expression
Primer sequences used for qRT-PCR
Gene | Primer sequences |
---|---|
SNHG16 | |
F | 5ʹ-CAGAATGCCATGGTTTCCCC-3ʹ′ |
R | 5ʹ-TGGCAAGAGACTTCCTGAGG-3ʹ |
miR-302b-3p | |
Loop | 5′-GTCGTATCCAGTCCAGGGACCGAGGACTGGATACGACCTACTA-3′ |
F | 5′-GCGTAAGTGCTTCCATGTT-3′ |
R | 5′-TCCAGGGACCGAGGA-3′ |
SLC2A4 | |
F | 5′-TGGCTGGGTTCTCCAACTG-3′ |
R | 5′-CTGGAAACCCATGCCAATG-3′ |
β-actin | |
F | 5′-ACCGAGCGCGGCTACAG-3′ |
R | 5′-CTTAATGTCACGCACGATTTCC-3′ |
U6 snRNA | |
F | 5′ -CTCGCTTCGGCAGCACA-3′ |
R | 5′-AACGCTTCACGAATTTGCGT-3′ |
Quantitative Analysis of SNHG16, SLC2A4 and miR-302b-3p Expression
Table 1 Primer sequences for qRT-PCR
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