Polysorbate 20

Manufactured by Bio-Rad

Polysorbate 20 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a wetting agent, emulsifier, and detergent, helping to solubilize and disperse materials in aqueous solutions.

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Lab products found in correlation

Non fat dry milk, by Bio-Rad (1 mentions) Silicon chips, by Ted Pella (1 mentions) Bovine albumin, by MP Biomedicals (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)

2 protocols using polysorbate 20

Quantifying RBD-ACE2 Binding Kinetics

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Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s. Biosensors with captured RBD were washed in assay buffer, dipped in the indicated concentrations of sACE2–8h protein, and returned to assay buffer to measure dissociation. Data were collected on a BLItz instrument and analyzed with a 1:1 binding model using BLItz Pro Data Analysis Software (Molecular Devices). The assay buffer was 10 mM HEPES pH 7.6, 150 mM NaCl, 3 mM EDTA, 0.05% polysorbate 20, 0.5% non-fat dry milk (Bio-Rad).

, & Procko E. (2020). The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. bioRxiv.

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Raman Spectroscopy for Biomolecular Reference Spectra

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Silicon chips (5×5mm; Ted Pella, Inc., Redding, CA) served as substrates for all Raman measurements performed herein. The following reagents were used to generate the reference spectra library: bovine albumin (MP Biomedicals; Solon, OH), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC; Avanti Polar Lipids, Inc., Alabaster, AL), 1,2-dioleoyl-sn¬-glycero-3-phsophocholine (DOPC; Avanti Polar Lipids, Inc., Alabaster, AL), DNA (from salmon sperm; AmResco Inc., Solon, OH), and cholesterol (ovine; Avanti Polar Lipid, Inc., Alabaster, AL). Polysorbate 20 (Bio-Rad Laboratories, Inc., Hercules, CA) was utilized as surfactant in diluent for albumin ink formulations. The following reagents were used to formulate HDL nanoparticle suspensions: 22A (PVLDLFRELLNELLEALKQKLK) was synthesized by Genscript (Piscataway, NJ), using solid-phase Fmoc (9-fluorenylmethyl carbamate) chemistry and purified with reverse phase chromatography (>95 % pure). 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was purchased from NOF America Corporation and cholesterol was obtained from Sigma-Aldrich (St. Louis, MO).

LaLone V., Fawaz M.V., Morales-Mercado J., Mourão M.A., Snyder C.S., Kim S.Y., Lieberman A.P., Tuteja A., Mehta G., Standiford T.J., Raghavendran K., Shedden K., Schwendeman A., Stringer K.A, & Rosania G.R. (2019). Inkjet-Printed Micro-Calibration Standards for Ultraquantitative Raman Spectral Cytometry. The Analyst, 144(12), 3790-3799.

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