Lsm780 confocal system

Manufactured by Zeiss

Sourced in Germany

The LSM780 confocal system is a high-performance microscopy solution designed for advanced imaging applications. It offers a comprehensive set of features and capabilities that enable researchers to conduct detailed analyses of biological samples. The system's core function is to provide high-resolution, three-dimensional imaging of fluorescently labeled specimens through the use of confocal laser scanning microscopy technology.

Automatically generated - may contain errors

Lab products found in correlation

Zen 2012, by Zeiss (5 mentions) Axio imager, by Zeiss (4 mentions) Oct compound, by Sakura Finetek (3 mentions) Superblock blocking buffer, by Thermo Fisher Scientific (2 mentions) Ab75813, by Abcam (2 mentions) Zf 0313, by Merck Group (2 mentions) Bz x700 microscope, by Keyence (2 mentions) Zen blue software, by Zeiss (2 mentions) Inverted microscope, by Zeiss (2 mentions) Formaldehyde, by Thermo Fisher Scientific (1 mentions) Anti gl7 alexa488, by BioLegend (1 mentions) Citrisolv, by Thermo Fisher Scientific (1 mentions) Catalog no ab181598, by Abcam (1 mentions) Catalog no 4583, by Sakura Finetek (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Sc301530, by Santa Cruz Biotechnology (1 mentions) Slowfade gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Ab4728, by Abcam (1 mentions) Ab52455, by Abcam (1 mentions) Zm 0071, by ZSGB-BIO (1 mentions)

37 protocols using lsm780 confocal system

Immunofluorescent Staining of Lung Tissue for Tertiary Lymphoid Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Left lobe of the whole lung was harvested and fixed in 10% formaldehyde (Thermo Fisher Scientific) until embedding. Fixed lung tissues were embedded in paraffin, sectioned at 10-μm thickness. To identify tertial lymphoid structure, the lung tissue slide was stained with hematoxylin and eosin by the Mayo Clinic Histology Core Laboratory (Scottsdale, AZ). To measured iBALT structure, and lung tissue sections were deparaffinized in CitriSolv (Thermo Fisher Scientific) for 30 min and then immersed in ethanol series from 100, 95, 85, and 75% to distill H₂O for 5 min each for tissue hydration. For Ag retrieval, hydrated sections were steamed for 20 min in 1 mM EDTA (pH 8.0). Lung sections were blocked with Super Block Blocking buffer (Thermo Fisher Scientific) for 1 hour at room temperature. Anti–B220-eflour660 (clone: 4SM95, Invitrogen), anti–CD4-eflour570 (clone: RA3-6B2, Invitrogen), and/or anti–GL7-Alexa488 (clone: GL7, BioLegend) were stained on the lung tissue sections overnight at 4°C. After washing in 0.1% PBST [phosphate-buffered saline (PBS) with Tween 20], the slides were counterstained with 4′, 6-diaminodino-2-phenylindole (DAPI) and mounted. Tissue staining was reviewed, and representative images were acquired on a Zeiss LSM 780 confocal system (Carl Zeiss).

Son Y.M., Cheon I.S., Wu Y., Li C., Wang Z., Gao X., Chen Y., Takahashi Y., Fu Y.X., Dent A.L., Kaplan M.H., Taylor J.J., Cui W, & Sun J. (2021). Tissue-resident CD4+ T helper cells assist the development of protective respiratory B and CD8+ T cell memory responses. Science immunology, 6(55), eabb6852.

+ Open protocol

+ Expand

Immunofluorescence and Immunohistochemical Analysis of Human Decidual Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human decidual tissues were briefly fixed in 4% paraformaldehyde (PFA) and embedded in O.C.T. Compound (Catalog No. 4583, Sakura Finetek, Torrance, CA). The frozen sections at 10 μm were further fixed in 4% PFA and treated with 0.1% triton, and subjected to the incubation with specific antibodies against NCAM1/CD56 (Catalog No. ab75813, Abcam, Cambridge, MA), CK7 (Catalog No. ab181598, Abcam), CD39 (Catalog No. 14211-1-AP, Proteintech, Wuhan, China), or CD103 (Catalog No. 350227, BioLegend). Binding of the antibody was visualized using FITC-conjugated or TRITC-conjugated secondary antibody (Catalog No. ZF-0311 or ZF-0313, ZSGB-BIO, Beijing, China), and cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; Catalog No. 28718-90-3, Sigma). Immunofluorescent staining was examined using Zeiss LSM780 confocal system (Carl Zeiss, Jena, Germany) and processed with ZEN 2012 software (Carl Zeiss). Immunohistochemical staining for CK in decidua was performed by using antibody against CK7 (Catalog No. ab181598, Abcam) and HRP-conjugated second antibody (Catalog No. PV-6001, ZSGB-BIO) followed by recovery of substrate diaminodbenzidine (DAB) (Catalog No. ZLI-9019, ZSGB-BIO). The imagines were recorded on a light microscope with charge-coupled device (CCD) (Olympus, Tokyo, Japan).

Wang F., Jia W., Fan M., Shao X., Li Z., Liu Y., Ma Y., Li Y.X., Li R., Tu Q, & Wang Y.L. (2021). Single-cell Immune Landscape of Human Recurrent Miscarriage. Genomics, Proteomics & Bioinformatics, 19(2), 208-222.

+ Open protocol

+ Expand

Myofibroblast Stress Fiber Visualization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Capturing myofibroblast stress fiber formation using immunofluorescence was accomplished similar to previous studies [36 (link)] by growing 1.75 × 10⁵ cells/well on glass coverslips in 6-well plates and treated as described in the scratch assay method above. Before immunofluorescent staining each well was washed 2x with PBS (5 minutes/wash), fixed with 4% paraformaldehyde in PBS (5 minutes at room temperature), washed again with PBS (2× 5 minutes), permeablized with 0.1% Triton X-100 in PBS (3 minutes at room temperature), and washed a final time in PBS (2× 5 minutes). Cells were then stained for both filamentous actin (1:40 dilution; 1 hour at room temperature) with tetrarhodamine isothiocyanate (TRITC)–labeled phalloidin (SC-301530; Santa Cruz) and nuclei with 4′,6-diamidino-2-phenylindole (1:500 dilution, DAPI; Molecular Probes, Eugene, OR). Coverslips were mounted onto slides using SlowFade Gold Antifade Mountant (S36937; ThermoFisher Scientific, Waltham, MA) and images collected on a Zeiss LSM 780 confocal system (Carl Zeiss, Jena, Germany) with a 40x water immersion objective with the same florescent parameters for all images. Assays were completed in technical triplicate and images relative to the average shown.

Hernandez D.M., Kang J.H., Choudhury M., Andrianifahanana M., Yin X., Limper A.H, & Leof E.B. (2020). IPF Pathogenesis is Dependent upon TGFβ Induction of IGF-1. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(4), 5363-5388.

+ Open protocol

+ Expand

Immunofluorescent Lung Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The left lobe of the whole lung was harvested and fixed in 4% paraformaldehyde solution overnight at 4°C. The fixed sample was sequentially incubated in 15 and 30% sucrose solutions in PBS for 12 h each. Subsequently, the sample was embedded with OCT compound (Sakura Finetek) and stored at –80°C. For Ab staining and immunofluorescence imaging, lung sections were blocked with SuperBlock blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature. B220 eFluor 660 (clone 4SM95, Invitrogen), CD4 eFluor 570 (clone RA3-6B2, Invitrogen), and/or GL7 Alexa Fluor 488 (clone GL7, BioLegend) Abs were stained on the lung tissue sections overnight at 4°C. After washing in 0.1% PBST (PBS with Tween 20), the slides were counterstained with DAPI and mounted. Tissue staining was observed, and representative images were captured using a Zeiss LSM 780 confocal system (Carl Zeiss).

Son Y.M., Cheon I.S., Li C, & Sun J. (2024). Persistent B Cell–Derived MHC Class II Signaling Is Required for the Optimal Maintenance of Tissue-Resident Helper T Cells. ImmunoHorizons, 8(2), 163-171.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Decidual and Villous Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human decidual or villous tissues were embedded in OCT compound (Sakura Finetek) and frozen sectioned at 8 µm. The frozen sections or the FACS‐isolated cells were briefly fixed in 4% PFA and treated with 0.1% triton, and subjected to the incubation with specific antibodies against HLA‐G (Abcam, ab52455, 1:500), NCAM1 (Abcam, ab75813, 1:300), FOXP3 (Abcam, ab4728, 1:200), Jagged 1 (Abcam, ab7771, 1:200) or cytokeratin 7 (CK7; ZSGB‐BIO, ZM‐0071, 1:200). Binding of the antibody was visualized using FITC‐conjugated or TRITC‐conjugated secondary antibody (ZSGB‐BIO, ZF‐0311, ZF‐0313, 1:100), and cell nuclei were stained with 4,6‐diamidino‐2‐phenylindole (DAPI; Sigma, 28718‐90‐3). The results were recorded using a Zeiss LSM780 confocal system (Zeiss) and processed with ZEN 2012 software (Zeiss). To quantify the number of Tregs in a unit area of SPA and IVS, the regions of SPA or IVS in one image view were lined out, and the pixels were counted and transformed to area (mm²) by Image‐Pro Plus 6.0 (Media Cybernetics). At least three random views were analysed for each section, and 10 cases each from healthy pregnancy or RSA group were randomly selected for analysis.

Ma Y., Yang Q., Fan M., Zhang L., Gu Y., Jia W., Li Z., Wang F., Li Y., Wang J., Li R., Shao X, & Wang Y. (2020). Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T‐cell differentiation along the maternal‐placental circulation. Cell Proliferation, 53(5), e12802.

+ Open protocol

+ Expand

Confocal Microscopy for Sensitized Emission FRET

Check if the same lab product or an alternative is used in the 5 most similar protocols

Confocal images were sequentially acquired with Zeiss Zen software on a Zeiss LSM 780 Confocal system (Carl Zeiss Inc,) with a Zeiss Observer Z1 inverted microscope and a 25 mW Argon visible laser tuned to 488 nm and a 15 mW laser tuned to 594 nm. A 40x Plan-Apochromat 1.4 NA oil immersion objective was used at various digital zoom settings. Emission signals after sequential excitation were collected, from 490 to 553 nm or from 598 to 696 nm respectively, using individual photomultipliers. For sensitized emission FRET, an additional FRET channel was collected with 488 nm excitation and 598 to 696 nm emissions. The same confocal parameters (laser intensity, detector gain) were used for all FRET samples. mCherry alone and GFP alone samples served as FRET negative controls. FRET images were analyzed with the Zeiss Zen FRET macro.

Kaur S., Chang T., Singh S.P., Lim L., Mannan P., Garfield S.H., Pendrak M.L., Pantoja D.R., Rosenberg A.Z., Jin S, & Roberts D.D. (2014). CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. Journal of immunology (Baltimore, Md. : 1950), 193(8), 3914-3924.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Human Decidual Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human decidual tissues were briefly fixed in 4% PFA and embedded in O.C.T. compound (Sakura Finetek, Torrance, CA, USA). The frozen sections at 10 um were further fixed in 4% PFA and treated with 0.1% triton, and subjected to the incubation with specific antibodies against NCAM1/CD56 (Abcam, ab75813, 1:300), CK7 (Abcam, ab181598, 1:10000), CD39 (proteintech, 14211-1-AP, 1:200), or CD103 (350227, Biolegend, 1; 200) . Binding of the antibody was visualized using FITC-conjugated or TRITC-conjugated secondary antibody (ZSGB-BIO, ZF-0311, ZF-0313, 1:100), and cell nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI; Sigma, 28718-90-3, St Louis, MO, USA).
The results were recorded using Zeiss LSM780 confocal system (Zeiss, preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted September 18, 2020. ; https://doi.org/10.1101/2020.09.16.300939 doi: bioRxiv preprint Oberkochen, BW, Germany) and processed with ZEN 2012 software (Zeiss).
Immunohistochemical staining for Cytokeratin (CK) in decidua was performed by using antibody against CK7 (ab181598, Abcam,1:10000) and HRP-conjugated second antibody (ZSGB-BIO, PV-6001) followed by recovery of substrate DAB (ZSGB-BIO, ZLI-9019). The imagines were recorded on a light microscope with CCD (DP72, Olympus, Japan).

Wang F., Jia W., Fan M., Li Z., Liu Y., Ma Y., Shao X., Li Y., Li R., Tu Q., & Wang Y. (2020). Single-cell immune landscape of human recurrent spontaneous abortion.

+ Open protocol

+ Expand

Detecting RNA using QUMA-1 probe

Check if the same lab product or an alternative is used in the 5 most similar protocols

SCs were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature. The fixed cells were then stained with 1 μM probe QUMA-1 for 20 min at 37 °C. For cells treated with RNase A, fixed cells were firstly permeabilized with 0.5% Triton X-100 in PBS at RT for 20 min followed by incubation with 100 μg/ml RNase A for 1 h at 37 °C and staining with 1 μM probe QUMA-1 for 20 min at 37 °C. After incubation with QUMA-1, SCs cells were washed three times with PBS and confocal images were taken using a Zeiss LSM-780 confocal system.

Chen X., Yuan J., Xue G., Campanario S., Wang D., Wang W., Mou X., Liew S.W., Umar M.I., Isern J., Zhao Y., He L., Li Y., Mann C.J., Yu X., Wang L., Perdiguero E., Chen W., Xue Y., Nagamine Y., Kwok C.K., Sun H., Muñoz-Cánoves P, & Wang H. (2021). Translational control by DHX36 binding to 5′UTR G-quadruplex is essential for muscle stem-cell regenerative functions. Nature Communications, 12, 5043.

+ Open protocol

+ Expand

Quantifying Neurogenic Niche Cells in Mouse Brain

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Coronal brain sections (40 μm) were immunostained using an antigen retrieval procedure as previously described [14 (link)]. Immunostaining was performed with the following primary antibodies: anti-nestin (Aves; chick; 1:500 dilution), and anti-MCM2 (BD; mouse; 1:500 dilution). 4',6-diamidino-2-phenylindole (DAPI; Sigma) was used for counterstaining. Images were acquired on a LSM 780 confocal system (Carl Zeiss) with X40 objective lens and multi-track configuration. Stereological quantification of SGZ MCM2⁺ and MCM2⁺nestin⁺ cells was carried out as previously described [14 (link)]. RGLs were considered MCM2⁺nestin⁺ cells with apical process. Non-RGLs were considered MCM2⁺nestin⁺ cells with small horizontal processes or no processes [9 (link),15 (link)]. An observer blind to the genotypes and treatment of the animals performed assessments. Statistical significance (P < 0.05) was assessed by one-way ANOVA.

Jun H., Mohammed Qasim Hussaini S., Cho C.H., Welby J, & Jang M.H. (2015). Gadd45b Mediates Electroconvulsive Shock Induced Proliferation of Hippocampal Neural Stem Cells. Brain stimulation, 8(6), 1021-1024.

+ Open protocol

+ Expand

Retinal Vasculature Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Images of HE sections of retinas were captured using an IX71 microscope (Olympus) and the retinal thickness of the peripheral area was measured using cellSens Standard (ver.1.4.1; Olympus). Images of the whole mount retinas were captured using a LSM780 confocal system (Carl Zeiss) and a BZ-X700 microscope (Keyence). Percentage of the immunoreactivity of CD31 and collagen type IV (pixel) in whole retinal area (pixel) were analyzed using BZ-H3C software (Keyence). For pericyte coverage on the retinal vasculature, the percentage of area that was immunoreactive (pixel) for NG2, CD13 and CD31 was analyzed using ImageJ (National Institutes of Health, Bethesda, MD, USA). For the analysis of western blotting data, the immunoreactive bands were quantified using ImageJ (NIH) and normalized to the β-actin band.

Kitahara H., Kajikawa S., Ishii Y., Yamamoto S., Hamashima T., Azuma E., Sato H., Matsushima T., Shibuya M., Shimada Y, & Sasahara M. (2018). The Novel Pathogenesis of Retinopathy Mediated by Multiple RTK Signals is Uncovered in Newly Developed Mouse Model. EBioMedicine, 31, 190-201.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!