To quantify the nicotine deposited in the ALI exposure systems, exposures were repeated as described in the previous protocol sections using isopropyl alcohol (IPA; Fisher Scientific, Fair Lawn, NJ, USA) in place of the biological samples. Ten mL of IPA were added to each exposure well in both the cloud chamber and Cultex® system. After the exposures, the IPA was collected and shipped overnight on ice to Portland State University where it was analyzed using gas chromatography-mass spectrometry (GC-MS). Chemical analysis was performed with an Agilent 5975 GC/MS system (Agilent, Santa Clara, CA, USA) using an internal standard-based calibration procedure and method previously described [25 (link), 50 (link)].
5975 gc ms system
Manufactured by Agilent Technologies
Sourced in United States
The 5975 GC-MS system is a gas chromatography-mass spectrometry instrument manufactured by Agilent Technologies. It is designed to perform qualitative and quantitative analysis of complex mixtures by separating and identifying individual chemical components.
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5 protocols using 5975 gc ms system
1
Nicotine Quantification in ALI Exposures
2
Quantifying Nicotine Deposition in ALI Exposure
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To quantify the nicotine deposited in the ALI exposure systems, exposures were repeated as described in the previous protocol sections using isopropyl alcohol (IPA; Fisher Scientific, Fair Lawn, NJ, USA) in place of the biological samples. Ten mL of IPA were added to each exposure well in both the cloud chamber and Cultex system. After the exposures, the IPA was collected and shipped overnight on ice to Portland State University where it was analyzed using gas chromatography-mass spectrometry (GC–MS). Chemical analysis was performed with an Agilent 5975 GC/MS system (Agilent, Santa Clara, CA, USA) using an internal standard-based calibration procedure and method previously described25 (link),51 (link).
3
Essential Oil Composition Analysis
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The essential oil composition was characterized by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrophotometry (GC-MS) techniques. GC-MS analysis was performed by using an Agilent 5975 GC-MS system coupled to an Agilent 7890 A GC. To separate chemical components, the HP-Innowax column (60 m × 0.25 mm, 0.25 μm film thickness) was used. Other analytical parameters were reported in our earlier paper [48 (link)].
The retention index (RI) calculated by co-injection with reference to a homologous series of n-alkanes (C8–C30) under the identical experimental circumstances was used to identify the components. In order to make more accurate identifications, RI values and mass spectra of the compounds were compared with those of the literature and NIST 05 and Wiley eighth edition, respectively.
The retention index (RI) calculated by co-injection with reference to a homologous series of n-alkanes (C8–C30) under the identical experimental circumstances was used to identify the components. In order to make more accurate identifications, RI values and mass spectra of the compounds were compared with those of the literature and NIST 05 and Wiley eighth edition, respectively.
4
Hydrodistillation of Essential Oil
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The essential oil was obtained by using the hydro-distillation technique. One hundred g dried plant samples were distilled in a Clevenger-type apparatus for 5 h. The essential oil was dried over sodium sulphate (anhydrous) and then the obtained essential oils were stored in an amber vial at +4 °C until analysis.
The obtained essential oil was characterized by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrophotometry (GC-MS) techniques. GC-MS analysis was performed by using a 5975 GC-MS system (Agilent, city, state abbreviation if USA, country) coupled to an Agilent 7890 A GC. To separate chemical components, a HP-Innowax column (60 m × 0.25 mm, 0.25 μm film thickness) was used. Other analytical parameters were reported in our earlier paper [52 (link)]. All analytical details are given in theSupplementary Materials .
The retention index (RI) calculated by co-injection with reference to a homologous series of n-alkanes (C8-C30) under identical experimental circumstances was used to identify the components. By comparing their mass spectra to those from the NIST 05 and Wiley 8th edition libraries, as well as comparing their RIs to literature values, we were able to make more accurate identifications.
The obtained essential oil was characterized by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrophotometry (GC-MS) techniques. GC-MS analysis was performed by using a 5975 GC-MS system (Agilent, city, state abbreviation if USA, country) coupled to an Agilent 7890 A GC. To separate chemical components, a HP-Innowax column (60 m × 0.25 mm, 0.25 μm film thickness) was used. Other analytical parameters were reported in our earlier paper [52 (link)]. All analytical details are given in the
The retention index (RI) calculated by co-injection with reference to a homologous series of n-alkanes (C8-C30) under identical experimental circumstances was used to identify the components. By comparing their mass spectra to those from the NIST 05 and Wiley 8th edition libraries, as well as comparing their RIs to literature values, we were able to make more accurate identifications.
5
GC-MS Analysis of Volatile Compounds
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The GC-MS analysis was carried out with an Agilent 5975 GC-MS system. Innowax FSC column (60m, 0.25mm film thickness) was used with helium as carrier gas (0.8ml/min). GC oven temperature was kept at 60°C for 10 min and programmed to 220°C at a rate of 4°C/min, and kept constant at 220°C for 10 min. Then, programmed to 240°C at a rate of 1°C/min. Split ratio was adjusted at 40:1. The injector temperature was set at 250°C. Mass spectra were recorded at 70eV. Mass range was from m/z 35 to 450.
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