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Anti phospho src

Manufactured by Santa Cruz Biotechnology

Anti-phospho-Src is a laboratory reagent that detects phosphorylated Src protein. It is a specific antibody used to identify the presence and quantify the levels of Src protein when it is in a phosphorylated state.

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2 protocols using anti phospho src

1

Quantitative Western Blotting of Phospho-Src

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Western blotting was performed with anti-phospho-Src (Tyr-416, cell signaling), anti-Lyn, and anti-Hck (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) primary antibodies and with relevant secondary antibodies (Santa Cruz Biotechnology, Inc.) via standard techniques [11 (link)]. Phospho-Src band intensities were determined densitometrically with Image J software, and normalized to Lyn or Hck intensity.
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2

Glycated-BSA-induced AGE signaling

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Glycated-BSA, used as the AGE in this study, was purchased from Bio Vision. Human thrombin was obtained from Sigma-Aldrich. Prostaglandin E1 (PGE1), anti-c-Src and anti-phospho-Src antibodies were purchased from Santa Cruz Biotechnology. BAPTA-AM and PP2 were obtained from Dojin and Abcam, respectively. Anti-Syk antibody and anti-phosphotyrosine antibody (PY100) were purchased from Cell Signaling Technology.
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