Anti cd49b antibody

For scRNA-seq in Fig. 1, BMBAs generated by culturing bone marrow cells in the presence of IL-3 (0.3 ng/mL: BioLegend) for 7 days were subjected to scRNA-seq analysis. BMBAs generated from 5 mice were pooled to generate scRNA-seq data. For scRNA-seq in Fig. 2, single-cell samples were prepared from the bone marrow and spleen of Mcpt8^GFP transgenic mice and depleted of lineage-positive cells by using biotinylated lineage antibodies (anti-CD4, anti-CD8a, anti-CD19, anti-B220, anti-Gr-1 and anti-TER-119, dilution 1:400) and EasySep Mouse Streptavidin RapidSpheres Isolation Kit (Stemcell Technologies, catalog #: ST-19860). GFP-positive cells were sort-purified from lineage-negative cells. Basophils isolated from 5 mice were pooled to generate scRNA-seq data. For scRNA-seq in Fig. 5, single-cell suspensions were prepared from the bone marrow and infected skin and subjected to positive selection of CD49b-positive cells by using biotinylated anti-CD49b antibody (BioLegend; clone: DX5, catalog #: 108904, dilution 1:400) and EasySep mouse Biotin Positive Selection Kit II (Stemcell Technologies, catalog #: ST-17665) followed by the isolation of CD200R3⁺cKit^- cells by cell sorting. Basophils isolated from 5 mice were pooled to generate scRNA-seq data.