Cd45 pe cy5

Manufactured by Santa Cruz Biotechnology

Sourced in United States

CD45-PE-cy5 is a fluorescently labeled antibody that binds to the CD45 cell surface antigen. It can be used to identify and quantify cells expressing CD45, a protein tyrosine phosphatase found on the surface of most hematopoietic cells.

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2 protocols using cd45 pe cy5

Flow Cytometry Analysis of Immune Cells

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The colon tissues were digested with 0.1% collagenase type 1 and 0.05% trypsin (Sigma-Aldrich, USA) for 30 min at 37°C. The cell suspensions were passed through a 70 μm cell strainer, collected, and incubated with CD45-PE-cy5, F4/80-FITC, CD86-PE, and CD206-PE (Santa Cruz Biotechnology, USA). Then, the cells were washed and analysed using flow cytometry with a FACS Calibur (BD Biosciences, USA). The gate was set on the CD45⁺ population, and surface markers were further analysed in this gate using Flowjo program (Tree Star, Ashland, OR, USA).

Wu X., Wu D., Mu Y., Zhao Y, & Ma Z. (2020). Serum-Free Medium Enhances the Therapeutic Effects of Umbilical Cord Mesenchymal Stromal Cells on a Murine Model for Acute Colitis. Frontiers in Bioengineering and Biotechnology, 8, 586.

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Comprehensive Antibody Characterization Panel

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We purchased antibodies against Ki67 (Clone: MM1), Villin (Santa Cruz, sc-7672), Gapdh (Clone: 6C5), Calnexin (Clone: 37), GM130 (Clone: 35), Adenine nucleotide translocase (Santa Cruz, sc-9299), Lyn (Santa Cruz, sc-15), GP130 (Santa Cruz, sc-9045), Tubulin (Clone: B-5-1-2), EpCAM-PE (Clone: 9C4), CD24-APC (Clone: ML5), CD45-PE-Cy5 (Clone: 30-F11), and PDI (Clone, 1D3). We generated the antibody against mouse ZIP7 by immunizing a rabbit with a peptide corresponding to the GNTGPRDGPVKPQSPEE sequence of mouse ZIP7, which was affinity-purified by using the antigen peptide.

Ohashi W., Kimura S., Iwanaga T., Furusawa Y., Irié T., Izumi H., Watanabe T., Hijikata A., Hara T., Ohara O., Koseki H., Sato T., Robine S., Mori H., Hattori Y., Watarai H., Mishima K., Ohno H., Hase K, & Fukada T. (2016). Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress. PLoS Genetics, 12(10), e1006349.

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