Clinical data were retrospectively investigated using medical records, and quantitative values were measured at the same time as sICs/cytokines estimation in the first and second weeks. Blood urine nitrogen (BUN), creatinine, lactate dehydrogenase (LD), procalcitonin, and C-reactive protein (CRP) levels were determined using an Atellica IM (Siemens Healthcare Diagnostics Manufacturing Ltd., Munich, Germany). The hemoglobin, platelet, total white blood cell (WBC), absolute neutrophil count (ANC), and lymphocyte counts were obtained using an ADVIA 2120i (Siemens Healthcare Diagnostics Manufacturing Ltd.). SARS-CoV-2 RNA was extracted using the MagNa Pure 96 System (Roche Diagnostics, Basel, Switzerland) and subjected to a real-time polymerase chain reaction using the STANDARD M nCoV Real-Time Detection kit (SD Biosensor, Gyeonggi, South Korea) and Bio–Rad CFX96 analyzer (Bio–Rad Laboratories) for quantification.
Atellica im
Manufactured by Siemens
Sourced in Germany
The Atellica IM is a laboratory instrument designed for in-vitro diagnostic testing. It is capable of performing immunoassay analysis on a variety of biological samples. The core function of the Atellica IM is to automate the process of immunoassay testing, providing accurate and reliable results to support clinical decision-making.
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Lab products found in correlation
Cfx96 analyzer, by Bio-Rad (1 mentions)
Standard m ncov real time detection kit, by SD Biosensor (1 mentions)
Magna pure 96 system, by Roche (1 mentions)
Atellica ch analyzer, by Siemens (1 mentions)
Ph meter ph 700, by Thermo Fisher Scientific (1 mentions)
Dimension xpand plus, by Siemens (1 mentions)
Bn 2, by Siemens (1 mentions)
Cobas e411, by Roche (1 mentions)
Alinity i, by Abbott (1 mentions)
Access dxl, by Beckman Coulter (1 mentions)
Cobas e801, by Roche (1 mentions)
8 protocols using atellica im
1
Comprehensive COVID-19 Laboratory Evaluation
2
Fecal Elastase and Metabolic Markers
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Blood samples were collected on the morning of the MRI after an overnight fast. All blood analyses except C-peptide and pro-insulin were performed by the clinical chemistry library of the Amsterdam UMC upon blood withdrawal. Separate blood tubes were processed for storage at -80 °C to be analyzed at a later date. C-peptide was measured in stored heparinized plasma using an immunoluminometric assay on an automated immunoanalyzer (Atellica IM, Siemens). Pro-insulin was measured in stored EDTA plasma using the Human Total Proinsulin ELISA kit by Millipore. Moreover, patients collected morning stool samples which were stored at -80 °C until the measurement of fecal elastase levels by ELISA (BIOSERV Diagnostics). Patients were categorized based on fecal elastase results into either normal exocrine function (≥200 μg/g) or exocrine insufficiency (<200 μg/g).
3
Comprehensive Clinical Chemistry Analysis
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Concentrations of sodium, potassium and chloride were measured potentiometrically using an ion-selective electrode on the Atellica CH Analyzer (Siemens Healthineers). Determinations of magnesium (xylidyl blue method), phosphate (ammonium molybdate method), calcium (o-cresol phthalein complexone method), glucose (hexokinase method), total protein (pyrogallol red method), albumin (bromocresol green method) were performed photometrically on the Atellica CH Analyzer (Siemens Healthineers). Immunoturbidimetric methods were used in the measurements of Lp(a), Apo A1, and Apo B on the Atellica CH Analyzer (Siemens Healthineers). Immunonephelometric concentration determinations of IgG, IgA, IgM, the IgG subclasses, α1-antitrypsin, α2-macroglobulin and transferrin were performed on the BN-II (Siemens Healthineers). Measurements of IgE-activity and concentrations of CA 125, CA 15 − 3, CA 19 − 9, and CA 72 − 4 were performed via chemiluminescent sandwich immunoassays on the Atellica IM (Siemens Healthineers). Ammonia concentrations were determined on Dimension XPand Plus (Siemens Healthineers) by enzymatic method using glutamate dehydrogenase. Measurements of pH values were performed on the pH meter pH700 (Eutech Instruments). Controls of the instruments were performed according to the manufacturer’s instructions. Parameters are accredited according DIN EN ISO 15189:2014.
4
Thyroid Hormone Assay Performance
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Thyroid hormone profiles, including TSH, T3, and free T4 (FT4) levels, were measured by quantitative chemiluminescent immunoassays using the Atellica IM analyzer (Siemens Healthineers, Erlangen, Germany). The assay showed TSH, T3, and FT4 levels of 0.008–150 mIU/L, 0.10–8.00 ng/mL, and 0.1–12.0 ng/dL, respectively. The TSH assay was designed to have within-laboratory precision of ≤0.0032 mIU/L standard deviation (SD) for samples <0.020 mIU/L, ≤16% coefficient of variation (CV) for samples ranging from 0.020 to 0.299 mIU/L, ≤8% CV for samples ranging from 0.300 to 90.00 mIU/L, and ≤10% CV for samples >90.00 mIU/L. The T3 assay was designed to have within-laboratory precision of ≤0.08 ng/mL SD for samples <0.60 ng/mL, ≤13% CV for samples ranging from 0.60 to 0.80 ng/mL, ≥11% CV for samples ranging from 0.80 to 1.80 ng/mL, and ≤10% CV for samples >1.80 ng/mL. The FT4 assay was designed to have within laboratory precision of ≤0.03 SD for samples <0.5 ng/dL, ≤8.0% CV for samples ranging from 0.5 to 1.0 ng/dL, and <6.0% CV for samples >1.0 ng/dL. The reference ranges of TSH, T3, and FT4 were 0.55–4.78 mIU/L, 0.6–1.8 ng/mL, and 0.89–1.76 ng/dL, respectively, as recommended by the kit provider.
5
Thyroid Hormones and Bone Mineral Density
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The T3, FT4, and TSH levels were determined using the blood sample obtained on the same morning that the BMD was measured. The serum T3, FT4, and TSH levels were measured by performing a direct chemiluminescent immunoassay (Atellica IM, Siemens Healthcare Diagnostics Inc.®, Malvern, PA, USA) with inter-assay coefficients of variation of less than 13%, 8%, and 10% for T3, FT4, and TSH, respectively. Subsequently, the T3, FT4, and TSH levels were subcategorized into either the high/normal/low or normal/abnormal based on the age-matched reference range (0.83–2.13 ng/mL for T3, 0.8–2 ng/dL for FT4, and 0.6–8 and 0.6–6 µU/mL for TSH of individuals aged 10–15 years and 16–18 years, respectively), and the LSBMD z-scores of each group were compared [16 (link)].
6
Vitamin D Status in COVID-19 Patients
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We tested total serum 25(OH)D levels to determine vitamin D status at the time of admission for COVID-19 patients with different disease severity. Serum 25(OH)D levels in the plasma were measured using automated electrochemiluminescence (Atellica IM, Siemens, Germany). Atellica IM VitD assay has a correlation coefficient of 0.95 and a slope of 1.00.1. (Atellica IM VitD, Siemens, Germany). Serum 25(OH)D levels were classified with cut-off value into sufficient (>20 ng/mL), insufficient (12–20 ng/mL), and deficient (<12 ng/mL).
7
Cushing's Syndrome Diagnostic Evaluation
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Thirty-six patients (25 females, 11 males; age: 48.58 ± 14.34 years) with confirmed HC [13 Cushing's disease, 8 ectopic ACTH-production, 15 adrenal Cushing's syndrome (of these 7 metastasized adrenocortical carcinomas)] were enrolled. These patients were referred to our centre with clinically overt HC. Most clinical signs and symptoms of HC were hypertension ≥ WHO grade II (prevalence: 69 %), weight gain/obesity (prevalence: 65 %), skin complaints (prevalence: 59 %) or proximal myopathy (prevalence: 43 %). The biochemical diagnosis for HC was established by at least two of the following parameters: elevated 24-hour urinary-free cortisol levels [measured by electrochemiluminescence immunoassay (ECLIA, Cobas e411, Roche Diagnostics, Rotkreuz, Switzerland)] in at least two measurements, elevated midnight serum cortisol (measured by ECLIA, Atellica IM, Siemens Healthcare, Erlangen, Germany) and insufficient serum cortisol suppression during a 1 mg dexamethasone suppression test. A final diagnosis of the cause of HC was established based on measurement of ACTH and in case of an ACTH-depending hypercortisolism due to the results of inferior petrosus sinus sampling (IPSS). ACTH-producing pituitary adenomas and ectopic ACTH-production as well as adrenal adenomas were proven histologically.
8
Comparative Evaluation of PSA Assays
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Samples' preparation and measurements on the four analytical platforms Access Dxl (Beckman Coulter), Atellica IM (Siemens Healthcare Diagnostics), Alinity i (Abbott Diagnostics) and Cobas e801 (Roche Diagnostics) have been described elsewhere [4] . Also, the assay design and the characterization of the antibodies used were reported in the same paper [4] .
Briefly, we selected 135 and 137 leftover serum samples, from different patients, as a representative clinical sampling for the measurement of tPSA and fPSA, respectively of the daily clinical routine, excluding from the collection haemolyzed, lipemic or icteric samples, according to the interference thresholds reported by PSA assays. The leftover serum specimens were immediately separated into a total of four 400 µL aliquots (one for each of the analytical platforms evaluated in the study) and stored at -80 °C until analysis. The study design followed the Clinical and Laboratory Standards Institute EP-09 protocol concerning the procedure of sample collection and measurements [17] .
Briefly, we selected 135 and 137 leftover serum samples, from different patients, as a representative clinical sampling for the measurement of tPSA and fPSA, respectively of the daily clinical routine, excluding from the collection haemolyzed, lipemic or icteric samples, according to the interference thresholds reported by PSA assays. The leftover serum specimens were immediately separated into a total of four 400 µL aliquots (one for each of the analytical platforms evaluated in the study) and stored at -80 °C until analysis. The study design followed the Clinical and Laboratory Standards Institute EP-09 protocol concerning the procedure of sample collection and measurements [17] .
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