Human scd14 immunoassay

The CSF analyses of T-tau (INNOTEST^®hTau Ag) and phospho-tau181 (P-tau181) (INNOTEST^® PHOSPHO-TAU (181P)) were performed using Innogenetics enzyme-linked immunosorbent assays (ELISA, Innogenetics, Ghent, Belgium). The NFL ELISA (UmanDiagnostics NF-light^®, Umeå, Sweden) was performed according to a previously established protocol,24 (link) with minor modifications. The analysis of MBP was performed by an ELISA (Active^® MBP, Diagnostic Systems Laboratories, Webster, TX, USA) purchased from Diagnostic Systems Laboratories. CSF levels of YKL-40 and sCD14 were analyzed by ELISAs (Quantikine^® ELISA, Human Chitinase 3-like 1 Immunoassay and Human sCD14 Immunoassay, both from R&D Systems Inc, Abingdon, UK). MCP-1 was measured by an ultra-sensitive ELISA (Human MCP-1 Ultra-Sensitive kit, Meso Scale Discovery, Rockville, MD, USA). All the commercial assays were analyzed according to the instructions given by the manufacturers. GFAP was measured by a previously described ELISA procedure.25 (link) The coefficients of variation for all biochemical analyses were below 10%.