GPC analysis was performed on a PL-GPC 50 integrated GPC/SEC system (Agilent, Basel, Switzerland) equipped with a refractive index detector, a PSS NOVEMA MAX columns set (1× guard column, 10 μm; 2× analytical columns 1000 Å, 10 μm; 1× analytical column 30 Å, 10 μm), using 0.3 M aqueous AcOH and the 0.2 M aqueous NaOAc as eluents. The system was calibrated with pullulan from 180 to 1,220,000 Da. The samples of CS and dCS (2 mg each) were dissolved overnight in 1.5 mL of mobile phase. The solutions were filtered through a sterile 0.22 µm PTFE filter followed by injection (100 µL). The flow rate of analysis was 1 mL/min at 40 °C. The results of the GPC analysis were expressed as the number of the average molecular weight (Mn), the weight average of the molecular weight (Mw), and the molecular weight of the highest peak (Mp) and polydispersity index (PD).
Pl gpc 50 integrated gpc sec system
Manufactured by Agilent Technologies
The PL-GPC 50 Integrated GPC/SEC System is a laboratory equipment designed for gel permeation chromatography (GPC) and size exclusion chromatography (SEC) analysis. It serves as an integrated solution for the characterization of polymers, small molecules, and other macromolecular species.
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4 protocols using pl gpc 50 integrated gpc sec system
1
Gel Permeation Chromatography of Chitosan
2
Polymer Characterization by GPC and HRTEM
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The molecular weights of the resulted polymers and oligomers were analyzed using PL-GPC 50 Integrated GPC/SEC System (Agilent Technologies) using a 1 mL/min THF flow rate and a column oven temperature of 30 °C using polystyrene as standard. The GPC traces were treated according to Gavrilov et al.37 (link).
The high-resolution transmission electron microscopy (HRTEM) studies were performed on an atomic resolution analytical JEOL JEM-ARM 200 F electron microscope, operating at 200 kV. Specimens for HRTEM were prepared in the following way: a drop of the solution was put on holey carbon TEM grid and dried at 100 °C for 5 min.
The high-resolution transmission electron microscopy (HRTEM) studies were performed on an atomic resolution analytical JEOL JEM-ARM 200 F electron microscope, operating at 200 kV. Specimens for HRTEM were prepared in the following way: a drop of the solution was put on holey carbon TEM grid and dried at 100 °C for 5 min.
3
Molecular Weight Analysis of Depolymerized PET
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2.3.1.GPC analysis of the glycolyzed PET.
Using gel permeation chromatography (GPC) and an Agilent Technologies PL-GPC 50 Integrated GPC/SEC System equipment fitted with a refractive index detector and tetrahydrofuran (THF) as solvent, the molecular weight of depolymerized PET was determined.
Using gel permeation chromatography (GPC) and an Agilent Technologies PL-GPC 50 Integrated GPC/SEC System equipment fitted with a refractive index detector and tetrahydrofuran (THF) as solvent, the molecular weight of depolymerized PET was determined.
4
Comprehensive Polymer Characterization by Advanced Analytical Techniques
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The UV-Vis-NIR spectra were recorded using a spectrofotometer Shimadzu UV-3600. The fluorescence spectra were registered using a Jasco FP-6500 Able Jasco spectrofluorimeter.
The molecular weights of the resulted polymers and oligomers were analyzed using PL-GPC 50 Integrated GPC/SEC System (Agilent Technologies) using a 1 mL/min THF flow rate and a column oven temperature of 50 °C.
Infrared absorption spectra have been recorded at room temperature with a Nicolet 6700 FTIR spectrometer in the range of 4000–400 cm−1.
The elemental analysis was performed with Perkin Elmer 2400 Series II CHNS/O Analyzer equipped with thermal conductivity detector (combustion temperature 975 °C, reduction temperature 500 °C).
The high resolution transmission electron microscopy (HRTEM) studies were performed on an atomic resolution analytical JEOL JEM-ARM 200F electron microscope, operating at 200 kV. Specimens for HRTEM were prepared in the following way: a drop of the solution was put on holey carbon TEM grid and dried at 100 °C for 5 min.
The molecular weights of the resulted polymers and oligomers were analyzed using PL-GPC 50 Integrated GPC/SEC System (Agilent Technologies) using a 1 mL/min THF flow rate and a column oven temperature of 50 °C.
Infrared absorption spectra have been recorded at room temperature with a Nicolet 6700 FTIR spectrometer in the range of 4000–400 cm−1.
The elemental analysis was performed with Perkin Elmer 2400 Series II CHNS/O Analyzer equipped with thermal conductivity detector (combustion temperature 975 °C, reduction temperature 500 °C).
The high resolution transmission electron microscopy (HRTEM) studies were performed on an atomic resolution analytical JEOL JEM-ARM 200F electron microscope, operating at 200 kV. Specimens for HRTEM were prepared in the following way: a drop of the solution was put on holey carbon TEM grid and dried at 100 °C for 5 min.
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