Lipopolysaccharides (lps)

Manufactured by MyBioSource

Sourced in United States

LPS is a laboratory equipment designed for the extraction and purification of lipopolysaccharides (LPS) from bacterial cultures. It utilizes a multi-step process to isolate LPS, a major component of the outer membrane of Gram-negative bacteria.

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Lab products found in correlation

8 protocols using lipopolysaccharides (lps)

Plasma Biomarkers in HIV-infected Individuals

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Plasma was isolated from blood samples collected into BD Vacutainer tubes and stored in endotoxin-free cryovials at −80°C. Behavioral data and blood samples typically were collected on the same day (range=0–17 intervening days). Enzyme-linked immunosorbent assays (ELISA) were used to quantify LPS (MyBioSource, San Diego, CA), LBP (Hycult Biotech, Wayne, PA), sCD14, and sCD163 (R&D Systems, Minneapolis, MN) following manufacturer instructions. ELISA tests were performed in duplicate, and samples with coefficient of variation ≥ 20% were excluded from further analysis. Percentages of samples exceeding this threshold by biomarker were as follows: 0% for LPS; 8% for LBP; 6% for sCD14; and 6% for sCD163. For HIV-infected participants, HCV status was determined at baseline by antibody test followed by HCV RNA testing for confirmation of reactive antibody results. HCV testing was not performed in controls due to resource limitations.

Monnig M.A., Cohen R., Ramratnam B., McAdams M., Tashima K, & Monti P.M. (2019). HIV Infection, HCV Co-Infection, and Alcohol Use: Associations with Microbial Translocation and Immune Activation. Alcoholism, clinical and experimental research, 43(6), 1126-1134.

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Plasma Biomarker ELISA Protocol

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Plasma ZO-1 (Catalog #: MBS706368, MyBioSource, San Diego, CA), LPS (Catalog #: MBS266722, MyBioSource, San Diego, CA), sCD14 (Catalog #: RK01060, Abyntek Biopharma, Vizcaya, Spain), and IL-1β (Catalog #: MBS2510385, MyBioSource, San Diego, CA) were measured using commercially available ELISA kits according to the manufacturers’ instructions. Circulating human anti-β-lactoglobulin antibody levels (IgG isotypes) were assayed using a validated home-made ELISA protocol as detailed below. All the plasma samples were measured in blind duplicates for each biomarker. For all protocols, absorbance at 450 nm with 570 nm correction was measured in a microplate reader, and corrected absorbance was interpolated in each standard curve to determine the concentrations (Sigma Plot).

Martín F., Blanco-Suárez M., Zambrano P., Cáceres O., Almirall M., Alegre-Martín J., Lobo B., González-Castro A.M., Santos J., Domingo J.C., Jurek J, & Castro-Marrero J. (2023). Increased gut permeability and bacterial translocation are associated with fibromyalgia and myalgic encephalomyelitis/chronic fatigue syndrome: implications for disease-related biomarker discovery. Frontiers in Immunology, 14, 1253121.

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Serum Biomarkers After Stroke

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Serum was collected from blood samples obtained after 2 days poststroke. ELISA assays were used to determine IGF (Insulin‐like Growth Factor)‐1 (R&D Laboratories), IGFBP‐3 (Crystal Chem), 17β‐estradiol, intestinal fatty acid binding protein (iFABP), LPS (My Biosource), following the manufacturer's protocol.

Bake S., Hurst D.A., Miranda R.C, & Sohrabji F. (2022). Prenatal alcohol exposure exacerbates acute sensorimotor deficits and impedes long‐term behavioral recovery from the effects of an adult‐onset cerebrovascular ischemic stroke. Alcoholism, Clinical and Experimental Research, 46(12), 2267-2279.

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Quantifying Endotoxin and Immune Markers

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Blood samples were collected into endotoxin-free tubes, processed, and stored at −80 °C. Commercially available enzyme-linked immunosorbent assays for LPS (MyBiosource), sCD14 (Enzo LifeSciences), and EndoCAb (Hycult) were performed in duplicate following manufacturer instructions. Data were available for 18/21 participants for LPS and 20/21 participants for sCD14 and EndoCAb.

Monnig M.A., Kahler C.W., Cioe P.A., Tucker L., Monti P.M., Mayer K.H, & Ramratnam B. (2016). Alcohol use predicts elevation in inflammatory marker soluble CD14 in men living with HIV. AIDS care, 28(11), 1434-1440.

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Quantifying Stress Biomarkers in Samples

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Serum concentrations of LPS (MBS700021; MyBioSource, San Diego, CA, USA), CORT (KA0468; Abnova, Neihu District, Taipei City, Taiwan), CRH (MBS265444; MyBioSource), ACTH (MBS2700344; MyBioSource), IFN-γ (551,866; BD Bioscience, Franklin Lakes, NJ, USA), and IL-6 (555,240; BD Bioscience) were measured using ELISA kits according to the manufacturer’s protocols.

Cho D.E., Hong J.P., Kim Y., Sim J.Y., Kim H.S., Kim S.R., Lee B., Cho H.S., Cho I.H., Shin S., Yeom M., Kwon S.K., Lee I.S., Park H., Kim K, & Hahm D.H. (2024). Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis. Scientific Reports, 14, 6263.

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Quantification of Intestinal Immunoglobulins

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To quantify luminal Igs, small intestines and colon were cut open longitudinally and mucus and feces were scrapped out and suspended into 1ml of PBS. Luminal content were spun at 8000g for 5 minutes and the supernatant collected and stored at −80°C. LPS (MyBiosource), sIgA (Cloud-Clone Corp.), IgA (Bethyl), IgM (Bethyl), IgG (Bethyl) and Calprotectin (S100A8/S100A9 Heterodimer Duoset; R&D systems) concentrations were measured by ELISAs in the serum or luminal content according to the manufacturer instructions. For luminal Igs measurement, concentration was normalized to the mucus weight. Murine serum Zonulin concentrations were measured by using a mouse Zonulin ELISA kit (MyBioSource).

Rivas M.N., Wakita D., Franklin M.K., Carvalho T.T., Abolhesn A., Gomez A.C., Chen S., Lehman T.J., Sato K., Shibuya A., Fasano A., Kiyono H., Abe M., Tatsumoto N., Yamashita M., Crother T.R., Shimada K, & Arditi M. (2019). Intestinal permeability and IgA provoke immune vasculitis linked to cardiovascular inflammation. Immunity, 51(3), 508-521.e6.

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Evaluation of LPS-Induced Metabolic Effects

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LPS (Escherichia coli 055:B5) were obtained from Aladdin (Beijing, China). De Man, Rogosa, and Sharpe (MRS) was from Solarbio Ltd. (Solarbio, Beijing, China). Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), ALT, AST, MDA, T-AOC, SOD, GSH-Px, CAT, TNF-α, transforming growth factor (TGF)-β, IL-1β, IL-6, IL-17, IL-10, IL-22, and LPS kits were obtained from MY BioSource Co., Ltd. (San Diego, CA, USA).

Guo W., Mao B., Tang X., Zhang Q., Zhao J., Cui S, & Zhang H. (2022). Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis. Microorganisms, 10(7), 1321.

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Plasma Biomarker Measurement Protocol

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Plasma insulin (Millipore, Burlington, MA, USA) and lipopolysaccharide (LPS, MyBioSource, San Diego, CA, USA) concentrations were measured using rat/mouse ELISA kits. F2-isoprostane levels were measured by extraction of isoprostanes from plasma samples, adding a mixture of F2 isoprostanes as internal standard (Concordia University, Montreal, QC, Canada) for mass spectrometric quantification [27 (link),28 (link)]. Plasma levels of triglyceride (TG) and total cholesterol (TC) were assessed using commercial kits (Randox Laboratories, Crumlin, UK). Lipid peroxidation was estimated by measuring plasma malondialdehyde (MDA) concentration by HPLC as previously described [29 (link)].

Sauvé M.F., Feldman F., Koudoufio M., Ould-Chikh N.E., Ahmarani L., Sane A., N’Timbane T., El-Jalbout R., Patey N., Spahis S., Stintzi A., Delvin E, & Levy E. (2021). Glycomacropeptide for Management of Insulin Resistance and Liver Metabolic Perturbations. Biomedicines, 9(9), 1140.

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