Hdac3

Manufactured by Thermo Fisher Scientific

HDAC3 is a lab equipment product from Thermo Fisher Scientific. It is a histone deacetylase enzyme that plays a role in the regulation of gene expression. The core function of HDAC3 is to remove acetyl groups from histone proteins, which can lead to changes in chromatin structure and transcriptional activity.

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Lab products found in correlation

Hdac4, by Merck Group (1 mentions) Ab4729, by Abcam (1 mentions) Bml 210, by Merck Group (1 mentions) Mef2d, by BD (1 mentions) Tmp195, by MedChemExpress (1 mentions) Recombinant human leptin, by Thermo Fisher Scientific (1 mentions) Phospho stat3 ser727, by Cell Signaling Technology (1 mentions) Phospho smad1 5, by Cell Signaling Technology (1 mentions) Phospho stat5, by Cell Signaling Technology (1 mentions) Phospho stat3 tyr705, by Cell Signaling Technology (1 mentions) Acetyl stat3, by Cell Signaling Technology (1 mentions) Phospho jak2, by Cell Signaling Technology (1 mentions) Phospho jak1, by Cell Signaling Technology (1 mentions) Noggin, by R&D Systems (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions)

2 protocols using hdac3

Histone Deacetylase Inhibitor Screening

Cited 2 times

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The primary antibodies used were anti: MEF2D (BD Bioscience); MEF2A (C-21), (Santa Cruz Biotechnology); MEF2C (15 (link)); Actin (Sigma-Aldrich); HDAC4 (16 (link)); HDAC5 (17 (link)); HDAC7 (18 (link)); HDAC9 (19 (link)); H3K27ac (ab4729) and H3K9ac (ab4441) (Abcam); Histone H3 (H0164, Sigma-Aldrich) HDAC3 (PA5-29026, Invitrogen). The following chemicals were used: SAHA (Cayman Chemicals); TMP195 (MedChemExpress), BML-210 (Sigma-Aldrich). The PAOA derivatives MC2983, MC2984, MC2985 and MC2991 were synthetized. All new compounds had spectral (¹H NMR, ESI-MS) data in agreement with the structure. Full details of the syntheses will be reported elsewhere. NKL54 (N-(2-aminophenyl)-N'-[3-(trifluoromethyl)phenyl]heptanediamide) was synthesized by SIA Chemspace (Riga, Latvia).

Minisini M., Di Giorgio E., Kerschbamer E., Dalla E., Faggiani M., Franforte E., Meyer-Almes F.J., Ragno R., Antonini L., Mai A., Fiorentino F., Rotili D., Chinellato M., Perin S., Cendron L., Weichenberger C.X., Angelini A, & Brancolini C. (2022). Transcriptomic and genomic studies classify NKL54 as a histone deacetylase inhibitor with indirect influence on MEF2-dependent transcription. Nucleic Acids Research, 50(5), 2566-2586.

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Antibodies and siRNAs for Investigating Signaling Pathways

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Rabbit polyclonal antibodies (pAbs) against Smad1/5 and goat pAb against COX2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse mAbs against HDAC1, HDAC2, and HDAC3, and rabbit pAbs against HDAC4, HDAC6, HDAC7, phospho-Ser727-STAT3, phospho-Tyr705-STAT3, acetyl-STAT3, phospho-STAT5, STAT3, STAT5, ERK, phospho-ERK1/2, phospho-Smad1/5, phospho-JAK1, phospho-JAK2, JAK1, and JAK2 were purchased from Cell Signaling Technology (Beverly, MA). Mouse mAbs against Collagen II were purchased from Chemicon (Temecula, CA). The control siRNA and specific siRNAs of BMP-2, Smad1, Smad5, STAT3, STAT5, JAK1, JAK2, ERK1, ERK2, HDAC3, and HDAC4 were purchased from Invitrogen (Carlsbad, CA). Recombinant human leptin was purchased from PeproTech (Rocky Hill, NJ, USA) and Noggin was purchased from R & D Systems (Minneapolis, MN). BMP-2 ELISA KIT were obtained from “American Diagnostica” (Greenwich, CT, USA). HDAC inhibitor-TSA [21 –23 (link)] were purchased from Sigma (St Louis, MO). All other chemicals of reagent grade were obtained from Sigma.

Chang S.F., Hsieh R.Z., Huang K.C., Chang C.A., Chiu F.Y., Kuo H.C., Chen C.N, & Su Y.P. (2015). Upregulation of Bone Morphogenetic Protein-2 Synthesis and Consequent Collagen II Expression in Leptin-stimulated Human Chondrocytes. PLoS ONE, 10(12), e0144252.

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