E el 0047c

ELISA kit was applied to detect the contents of norepinephrine (NE) (E-EL-0047c, Elabscience, Wuhan, China, https://www.elabscience.cn), alanine aminotransfease (ALT) (E-EL-R1232km-1, Elabscience) and aspartate aminotransferase (AST) (E-EL-R0076c, Elabscience) in rat serum. The kit was taken out to stand for 30 min at room temperature. The standard group (6 concentrations), the blank group and the sample group to be tested were set. The samples were added as follows: 100 μL of the standard solution was added to the blank well in the standard group; 100 μL distilled water was added to the blank well. The remaining wells were filled with 100 μL of samples to be tested. 50 μL hydrocresol peroxide solution was added to each well of the standard group and the sample group to be tested. Then, the label board was sealed with sealing paper and incubated at 37°C for 1 hr. At the end of incubation, each well was filled with diluted detergent solution and stood for 15-30 sec. In addition, the label board was fully cleaned for 5 times and the paper was patted dry. 50 μL chromogenic solution A and 50 μL chromogenic solution B were added to each well, and reacted at 25-37°C in the dark for 15 min. Ultimately, 50 μL of termination fluid was added to terminate the reaction. OD value of each well at a wavelength of 450 nm was measured by Molecular Devices (Shanghai, China).