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Sc 484

Manufactured by Santa Cruz Biotechnology
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Sourced in Morocco, United States

The Sc-484 is a laboratory instrument designed for the analysis and detection of biological samples. It features advanced optics and sensitive detection capabilities to enable accurate and reliable measurements. The core function of the Sc-484 is to provide researchers with a versatile tool for their analytical needs.

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Lab products found in correlation

Ab5095, by Abcam (5 mentions) Ab5131, by Abcam (5 mentions) Actin sc 1615, by Santa Cruz Biotechnology (2 mentions) Ab78272, by Abcam (2 mentions) α tubulin 200 301 880, by Rockland Immunochemicals (2 mentions) Sc 9001, by Santa Cruz Biotechnology (2 mentions) Sc 28678, by Santa Cruz Biotechnology (2 mentions) Sc 899, by Santa Cruz Biotechnology (2 mentions) Ab9132, by Abcam (2 mentions) Ab4729, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Ab1761, by Abcam (1 mentions) Ab290, by Abcam (1 mentions) Sc 8338, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Quantitect sybr green pcr, by Qiagen (1 mentions) Rotor gene rg 3000, by Qiagen (1 mentions) Mab β actin, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-CDK9 (sc-484) (2 citations)

6 protocols using sc 484

1

Western Blot Analysis of Transcription

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Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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2

Western Blot Analysis of Transcription

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Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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3

Chromatin Immunoprecipitation: Antibody Panel

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GFP (ab290, 5 µl used; abcam, Cambridge, MA), BRD4 (10 µg; ref. 49 (link)), CDK9 (sc-8338, 10 µg; sc-484, 10 µg; both from Santa Cruz Biotechnology), H3 (ab1791, 5 µg; abcam), acetyl H3 (06–599, 5 µg; Millipore, Billerica, MA), H3 acetyl K27 (ab4729, 2 µg; abcam), acetyl H4 (06–866, 5 µg; Millipore), H4 acetyl K12 (ab1761, 5 µg; abcam), Pol II (pan) (sc-899, 5 µg; Santa Cruz Biotechnology), Ser2P Pol II (ab5095, 5 µg; abcam), Ser5P Pol 11 (ab5131, 5 µg; abcam), or normal rabbit IgG (12–370, 5 µg; Millipore).
Kanno T., Kanno Y., LeRoy G., Campos E., Sun H.W., Brooks S.R., Vahedi G., Heightman T.D., Garcia B.A., Reinberg D., Siebenlist U., O’Shea J.J, & Ozato K. (2014). BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation. Nature structural & molecular biology, 21(12), 1047-1057.
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4

Western Blotting Analysis of RNA Pol II

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Western blotting was carried out as described previously [16] (link) using proteins harvested from cells boiled in Laemmli buffer (50 mM Tris (pH6.8), 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% bromophenol blue) and antibodies against pol II diluted 1∶200 (sc-899, Santa Cruz), pol II CTD Ser2P diluted 1∶1000 (ab5095, Abcam), Ser5P diluted 1∶1000 (ab5131, Abcam), Ser7P diluted 1∶200 [29] (link), Tyr1P diluted 1∶200 (61383, Active motif), RPAP2 diluted 1∶1000 (17401-1-AP, Proteintech), CTCF diluted 1∶1000 (07–729, Millipore), Myc-tag diluted 1∶1000 (ab9132, Abcam), CDK9 diluted 1∶500 (sc-484, Santa Cruz) and α-tubulin diluted 1∶1000 (200-301-880, Tebu-bio).
Zaborowska J., Baumli S., Laitem C., O'Reilly D., Thomas P.H., O'Hare P, & Murphy S. (2014). Herpes Simplex Virus 1 (HSV-1) ICP22 Protein Directly Interacts with Cyclin-Dependent Kinase (CDK)9 to Inhibit RNA Polymerase II Transcription Elongation. PLoS ONE, 9(9), e107654.
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5

ChIP Analysis of Transcriptional Regulation

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HeLa cells were transfected with pcDNA3, Myc-ICP22 and Myc-193-256 vectors using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions before being subjected to ChIP analysis as detailed [43] (link). ChIP samples were analyzed by qRT-PCR using QuantiTect SYBR Green PCR (Qiagen) and Rotor-Gene RG-3000 (Corbett Research). Error bars indicate the standard deviation from at least three independent experiments. Final ChIP values are expressed as a percentage of the total DNA input after deduction of the signal obtained using rabbit IgG as a negative control. Antibodies against pol II (sc-899) and CDK9 (sc-484) were obtained from Santa Cruz Biotechnology. Antibodies against Ser2P (ab5095), Ser5P (ab5131) and the Myc-tag (ab9132) were obtained from Abcam. The sequences of primers are given in Table 1.
Zaborowska J., Baumli S., Laitem C., O'Reilly D., Thomas P.H., O'Hare P, & Murphy S. (2014). Herpes Simplex Virus 1 (HSV-1) ICP22 Protein Directly Interacts with Cyclin-Dependent Kinase (CDK)9 to Inhibit RNA Polymerase II Transcription Elongation. PLoS ONE, 9(9), e107654.
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6

Western Blot Analysis of Infected Cells

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Western blot analyses of infected and mock-infected HFFs were performed as previously described [27 (link),66 (link)]. Antibodies specific for CDK9 (sc-484, Santa Cruz Biotechnology, Dallas, TX, USA), mAb-CDK1 (sc-54, Santa Cruz Biotechnology), pAb CDK2 (Sc-163, Santa Cruz Biotechnology), pAb-CDK7 (sc-723, Santa Cruz Biotechnology), and mAb β-actin (A5441, Sigma Aldrich, St. Louis, MO, USA) were used with an appropriate secondary antibody to detect the respective proteins.
Hahn F., Hamilton S.T., Wangen C., Wild M., Kicuntod J., Brückner N., Follett J.E., Herrmann L., Kheimar A., Kaufer B.B., Rawlinson W.D., Tsogoeva S.B, & Marschall M. (2021). Development of a PROTAC-Based Targeting Strategy Provides a Mechanistically Unique Mode of Anti-Cytomegalovirus Activity. International Journal of Molecular Sciences, 22(23), 12858.
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