Ultraglutamine 20mm solution

The human monocytic leukemia cell line THP-1 was purchased from DSMZ (Germany). Growth media (RPMI 1640) and Ultraglutamine 20 mM solution were purchased from Lonza (Belgium). Foetal bovine serum (FBS), phorbol 12-myristate 13-acetate (PMA), pure ethanol and ultra-pure water (W3500) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Human Interleukin 4 (IL-4) was purchased from Macs-Miltenyi Biotec (Germany). Cell proliferation reagent WST-1 was purchased from Roche Diagnostics GmbH (Germany) and the dye reagent for the Bradford protein assay was purchased from Bio-Rad Laboratories GmbH (Germany). RNeasy Mini kit, RT first strand kit and RT SYBR Green Rox Master Mix were from Qiagen, (CA, USA). The 384 RT-Array custom was made by Sabiosciences (Qiagen Company, CA, USA). Arnica m. was produced by Boiron Laboratoires (Lyon, France) according to the French Homeopathic pharmacopoeia and provided as a first centesimal dilution of the whole hydroalcoholic extract (Mother Tincture, MT) in 30% ethanol/distilled water. The MT was characterised by a content of sesquiterpene lactones of 0.036% (W/V).