Selenium

Manufactured by Lonza

Sourced in United States

Selenium is a high-purity, essential trace element that plays a critical role in various cellular processes. It is a key component in the production of certain enzymes and proteins, and is necessary for the proper functioning of the immune system and thyroid gland. Selenium can be used in a wide range of laboratory applications, including as a nutritional supplement or as a component in specialized reagents and equipment.

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Lab products found in correlation

Transferrin, by Lonza (5 mentions) Insulin, by Lonza (5 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (3 mentions) Foetal bovine serum (fbs), by Lonza (2 mentions) Epidermal growth factor (egf), by BD (2 mentions) Hcc1954, by American Type Culture Collection (2 mentions) Hek293, by American Type Culture Collection (2 mentions) Bt474, by American Type Culture Collection (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Mycoalert mycoplasma detection kit, by Lonza (2 mentions) Epidermal growth factor (egf), by Lonza (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) Six well plate, by Corning (1 mentions) Rat tail collagen type 1, by Merck Group (1 mentions) Gentamicin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Lonza (1 mentions) Dmem f12, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Glutamine, by Biosera (1 mentions) Ascorbic acid, by Merck Group (1 mentions)

Spelling variants of this product

Insulin transferrin selenium (10 citations)

7 protocols using selenium

IPEC-J2 Intestinal Epithelial Cell Culture

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The non‐transformed porcine intestinal epithelial cell line IPEC‐J2, originally isolated from jejunal epithelia of a neonatal unsuckled piglet (Schierack et al., 2006 (link)), was a kind gift of Dr. Jody Gookin, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, NC, USA. IPEC‐J2 cells were grown and maintained in complete medium, which consisted of a 1:1 mixture of Dulbecco's modified eagle's medium and Ham's F‐12 Nutrient Mixture (DMEM/F12) (plain medium) supplemented with 5% foetal bovine serum (FBS), 5 μg/ml insulin, 5 μg/ml transferrin, 5 ng/ml selenium, 5 ng/ml epidermal growth factor and 1% penicillin–streptomycin (all from Lonza Group Ltd, Belgium). Cells were grown at 37°C in a humidified atmosphere of 5% CO₂.
IPEC‐J2 cells were seeded onto six‐well plates (Corning Inc., Corning, NY, USA), coated with 8 μg/cm² rat tail collagen type I (Sigma–Aldrich, Steinheim, Germany), at a density of 10⁶ cells/ml; the volume of complete medium was 2.5 ml. Cells could adhere for 24 h before being washed and re‐fed every other day.

Palócz O., Noszticzius Z., Kály‐Kullai K., Bradley E, & Csikó G. (2021). In vitro study of chlorine dioxide on porcine intestinal epithelial cell gene markers. Veterinary Medicine and Science, 8(2), 591-597.

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Cultivation and Maintenance of IPEC-J2 Cell Line

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The IPEC-J2 cell line was provided by Juan José Garrido from the Department of Genetics and Animal Breeding, University of Cordoba, Spain. IPEC-J2 cells were grown and maintained in DMEM/F-12 (Sigma-Aldrich, St. Louis, MO, USA), supplemented with fetal bovine serum (5% FBS; Lonza, Switzerland), glutamine (2 mM; Biosera), epidermal growth factor (5 ng/mL; BD Biosciences, San José, CA, USA), transferrin (10 μg/mL), insulin (10 μg/mL), selenium (10 ng/mL) (Lonza), and gentamicin (50 μg/mL; Sigma-Aldrich). Cells were incubated at 37 °C in a fully humidified atmosphere with 5% CO_2, until confluence. Cells were seeded in 6-well culture plates (TPP, Switzerland) (1.5 × 10⁵ cells per well) 72 h before the experiment, and cultured in DMEM/F-12 medium as mentioned above, but supplemented with hydrocortisone (0.28 μM; Sigma-Aldrich) and ascorbic acid (5 μg/mL; Sigma-Aldrich) to avoid preliminary cell-activation. At 24 h prior to the experiment, the cell medium was changed to DMEM/F-12 without supplementation (without FBS and gentamicin). The cultures were regularly controlled for the absence of mycoplasma contamination [13 (link)].

Kiššová Z., Tkáčiková Ľ., Mudroňová D, & Bhide M.R. (2022). Immunomodulatory Effect of Lactobacillus reuteri (Limosilactobacillus reuteri) and Its Exopolysaccharides Investigated on Epithelial Cell Line IPEC-J2 Challenged with Salmonella Typhimurium. Life, 12(12), 1955.

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Cell Culture Media Composition

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DMEM growth medium, containing l-glutamine, d-glucose, and pyruvate, was purchased from US Biological (Swampscott, MA, U.S.A.). Insulin, transferrin, and selenium (500×) were purchased from Lonza (Walkersville, MD, U.S.A.). Humulin R was from Eli Lilly (Indianapolis, IN, U.S.A.). All other reagents were from Sigma–Aldrich Corp. (St. Louis, MO, U.S.A.), unless indicated otherwise.

Gibb A.A., Lorkiewicz P.K., Zheng Y.T., Zhang X., Bhatnagar A., Jones S.P, & Hill B.G. (2017). Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochemical Journal, 474(16), 2785-2801.

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Breast Cancer Cell Culture and PDX Propagation

Cited 2 times

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HMT-3522 T4–2 breast cancer cells were propagated as previously described^{57 (link)}. Cells were embedded in rBM (Growth Factor Reduced Matrigel) for 12 days to generate spheroids (~100 μm)^{23 (link)}. Primary breast cancer NHRI-BC-008 cells were isolated from the surgical specimen of a patient with TNBC (Tung’s Metro-Harbor Hospital, Taichung, Taiwan) and propagated in IMDM (Invitrogen) supplemented with glutamine, insulin, transferrin, selenium (Lonza) and 20% FBS^{59 (link)}. MDA-MB-231, T47D, HEK-293, HCC-1954, BT-474, and 4T1 cells (American Type Culture Collection) were grown on tissue culture plastic in DMEM with 10% fetal bovine serum and antibiotics. Cell lines were tested for Mycoplasma (MycoAlert Mycoplasma Detection Kit; Lonza). All cell lines were derived with authenticated sources. HCI-001 and HCI-002 PDX fragments were acquired from B. Welm (Huntsman Cancer Institute, University of Utah, UT). The PDX line BCM 2665, was acquired from M. Lewis (Baylor College of Medicine, Houston, TX)^{22 (link)}. All PDOs were processed and cultivated in rBM^{20 ,25}. All human tissues were collected using protocols approved by the Institutional Review Boards.

Tsai K.K., Huang S.S., Northey J.J., Liao W.Y., Hsu C.C., Cheng L.H., Werner M.E., Chuu C.P., Chatterjee C., Lakins J.N, & Weaver V.M. (2022). Breast cancer patient-derived organoid screening implicates the repressor NCOR2 in cytotoxic stress response and anti-tumor immunity. Nature cancer, 3(6), 734-752.

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Breast Cancer Cell Culture and PDX Propagation

Cited 2 times

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IPEC-1 Cells Challenged with ETEC

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The IPEC-1 cell line was grown in 6-well culture plates in medium containing Dulbecco’s modified Eagle medium/F-12 (Sigma-Aldrich) supplemented with 5% foetal bovine serum (FBS; Lonza, Switzerland), 5 ng/mL epidermal growth factor (BD Biosciences, USA), 10 μg/mL insulin, 10 μg/mL transferrin, and 10 ng/mL selenium (Lonza) at 37 °C in a fully humidified atmosphere with 5% CO₂. Upon reaching 70% confluency, the cells were washed with sterile phosphate-buffered saline, and EPS (0.1 mg/mL, 1 mL each well) reconstituted in IPEC-1 cell medium without FBS was added. After 4 h of preincubation with the EPS, the cells were challenged with ETEC (multiplicity of infection: 50:1) without refreshing of the medium for 45 min. Subsequently, the monolayers were washed with sterile PBS and stored at −20 °C until further use.

Tkáčiková Ľ., Mochnáčová E., Tyagi P., Kiššová Z, & Bhide M. (2020). Comprehensive mapping of the cell response to E. coli infection in porcine intestinal epithelial cells pretreated with exopolysaccharide derived from Lactobacillus reuteri. Veterinary Research, 51, 49.

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Isolation and Propagation of Primary Hepatocellular Carcinoma Cells

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Human HCC specimens were excised from four HCC patients who received tumor resection at Kaohsiung Veterans General Hospital (KVGH), Taiwan (Table S2). The isolation of primary HCC cells is described in the Supplemental Experimental Procedures. The freshly isolated HCC cells were cultured in IMDM (Invitrogen) supplemented with glutamine, insulin, transferrin, selenium (all from Lonza), and 20% fetal bovine serum (FBS). Two of the primary cells (KVGH-80T and KVGH-90T cells) could be propagated for more than ten passages to permit subsequent functional and molecular studies. HuH-1 (HBV-related HCC) and HuH-7 (non-B, non-C HCC) cells (Japanese Collection of Research Bioresources; RRID: CVCL-2956) were propagated on tissue culture plastics in DMEM (Invitrogen) supplemented with 10% FBS.

Liao W.Y., Hsu C.C., Chan T.S., Yen C.J., Chen W.Y., Pan H.W, & Tsai K.K. (2020). Dishevelled 1-Regulated Superpotent Cancer Stem Cells Mediate Wnt Heterogeneity and Tumor Progression in Hepatocellular Carcinoma. Stem Cell Reports, 14(3), 462-477.

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