Spectra manager code of federal regulations cfr software version 2

FT-IR spectral data were acquired from the skin of gilthead sea bream fillets using a ZnSe 45° HATR crystal (PIKE Technologies, Madison, WI, USA) and an FT-IR 6200 JASCO spectrometer (Jasco Corp., Tokyo, Japan), with a triglycine sulphate detector and a Ge/KBr beam splitter. The collected spectra were analyzed using the Spectra Manager™ Code of Federal Regulations (CFR) software version 2 (Jasco Corp.). The FT-IR spectral data over the wavenumber range of 3100–2700 cm⁻¹ and 1800–900 cm⁻¹ were selected for further analysis [11 (link)].
In addition, multispectral images from the skin of gilthead sea bream fillets were acquired using the Videometer Lab apparatus (Videometer A/S, Hørsholm, Denmark). This instrument acquires multispectral images in 18 non-uniformly distributed wavelengths ranging from 405 to 970 nm [11 (link),28 (link)]. The advantage of this method is that it provides information in the visible and short NIR region and, at the same time, it uses the spatial information of each pixel. Image acquisition, segmentation, and model development have been detailed previously [12 (link),29 (link)]. After the analysis of the images, feature extraction included the mean reflectance values of the 18 wavelengths (±the standard deviation) that were further assessed using multivariate analysis.

FT-IR measurements were performed using a ZnSe 45 HATR (Horizontal Attenuated Total Reflectance) crystal (PIKE Technologies, Madison, Wisconsin, United States), and a FT-IR-6200 JASCO spectrometer (Jasco Corp., Tokyo, Japan). The measurement crystal shows a refractive index of 2.4 and a depth of penetration of 2.0 ​μm ​at 1000 ​cm^-1. Spectra were obtained at the wavenumber range of 4000 to 400 ​cm^-1 using Spectra Manager Code of Federal Regulations (CFR) software version 2 (Jasco Corp., Tokyo, Japan), by accumulating 100 scans with a resolution of 4 ​cm^-1 and a total integration time of 2 ​min.

FTIR spectral data were collected using a ZnSe 45° HATR (Horizontal Attenuated Total Reflectance) crystal (PIKE Technologies, Madison, Wisconsin, United States), and a FTIR-6200 JASCO spectrometer (Jasco Corp., Tokyo, Japan). The crystal used has a refractive index of 2.4 and a depth of penetration of 2.0 μm at 1000 cm⁻¹. Spectra were collected over the wavenumber range of 4000 to 400 cm⁻¹ using the Spectra Manager™ Code of Federal Regulations (CFR) software version 2 (Jasco Corp.), by accumulating 100 scans with a resolution of 4 cm⁻¹ and a total integration time of 2 min.
A small portion from each meat sample was transferred to the crystal plate, covered with a small piece of aluminum foil, and then pressed to ensure the best possible contact with the crystal. Prior to the measurements of the samples, reference spectra were acquired using the cleaned blank (no added sample) crystal. After each measurement, the crystal’s surface was cleaned, first with detergent and distilled water and then with analytical grade acetone, and dried using lint-free tissue. As previously practiced [15 (link)], the FTIR spectra that were ultimately used in further analyses were in the approximate wavenumber range of 1800 to 900 cm⁻¹.