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Crocetin

Manufactured by Yuanye Bio-Technology
Sourced in China

Crocetin is a carotenoid compound extracted from the Crocus sativus plant. It serves as a key functional component in various laboratory applications, providing a natural colorant and potential antioxidant properties. The core function of Crocetin is to act as a molecular marker and research tool for scientific investigations.

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2 protocols using crocetin

1

Crocetin and ATO Antioxidant Mechanism

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Crocetin with HPLC purity of ≥ 98% and arsenic trioxide (ATO, MW:197.84) were procured from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) detection kits were procured from Jiancheng Bioengineering Institute of Nanjing (Nanjing, Jiangsu, China). The ROS detection kit was procured from Wuhan Servicebio Technology, Co., Ltd (Wuhan, China). Antibodies specific for Nrf2 and NQO1 were procured from Bioworld Technology, Co., Ltd. (Nanjing, Jiangsu, China). HO-1 antibodies were obtained from Wuhan Sanying Biotechnology Co., Ltd (Wuhan, China). β-actin was procured from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd (Beijing, China). The IL-6, IL-1β, and TNF-α enzyme-linked immunosorbent assay (ELISA) kits were obtained from Wuhan Servicebio Technology CO., Ltd (Wuhan, China).
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2

Hepatoprotective Compounds Evaluation

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Standard compounds geniposide, gardenoside, genipin-1-gentiobioside, shanzhiside, chlorogenicacid, crocin-1, crocin-2, crocetin, geniposidic acid, and genipin with over 98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Cell Counting Kit-8 purchased from EnoGene technology Co., Ltd. (Nanjing, China). Annexin V-FITC/PI apoptosis detection kit purchased from BestBio technology Co., Ltd. (Shanghai, China). DAPI staining solution purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). TNF-α,IL-6, and NO enzyme-linked immuno sorbent assay (ELISA) kits (R&D, USA) were purchased from Changchun Bestgene Biotechnology Co., Ltd. (Changchun, China). ALT, AST, ALP, MDA, SOD, and GSH kits were obtained from Nanjing JianCheng Institute of Biological Engineering (Nanjing, China). Acetonitrile and Formic acid (Fisher, Fair Lawn, NJ, USA) were of HPLC grade.
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