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Sp5 dm6000

Manufactured by Leica
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The Leica SP5 DM6000 is a high-performance confocal microscope designed for advanced imaging applications. It features a modular design, allowing for customization to fit specific research needs. The microscope offers a range of imaging capabilities, including fluorescence, phase contrast, and DIC imaging.

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Lab products found in correlation

Las af 3, by Leica (2 mentions)

Close spelling variants of this product

DM6000-CFS TCS-SP5 SP5/DM6000 confocal microscope TCS SP5 (DM6000 CS) DM6000

2 protocols using sp5 dm6000

1

Imaging Light Response in Apical Hook

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dark-grown pDR5::GFP/RFP seedlings in WT and different mutant backgrounds were imaged during the formation phase (15h), maintenance phase (40h), opening phase (60h) as well as after 1, 2 and 3 hours of light exposure (on apical hook in maintenance phase (40h)) using a SP5 Leica DM6000 confocal laser scanning microscope equipped with a 20.0x1.25 WATER objective. GFP (excitation 488 nm and emission 500 nm - 546 nm) and RFP (excitation 561 nm and emission 574 nm – 618 nm) were quantified on 3D maximum projection obtained from a Z-stack series of pictures taken in the inner side of the apical hook using the Leica software LAS AF 3.1. The same region of interest (ROI) was defined for each individual seedling. Sample size is given in the figure legends and the data was confirmed in 3 independent experiments. Representative experiments are shown.
Béziat C., Barbez E., Feraru M.I., Lucyshyn D, & Kleine-Vehn J. (2017). Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition. Nature plants, 3, 17105.
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2

Imaging Light Response in Apical Hook

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dark-grown pDR5::GFP/RFP seedlings in WT and different mutant backgrounds were imaged during the formation phase (15h), maintenance phase (40h), opening phase (60h) as well as after 1, 2 and 3 hours of light exposure (on apical hook in maintenance phase (40h)) using a SP5 Leica DM6000 confocal laser scanning microscope equipped with a 20.0x1.25 WATER objective. GFP (excitation 488 nm and emission 500 nm - 546 nm) and RFP (excitation 561 nm and emission 574 nm – 618 nm) were quantified on 3D maximum projection obtained from a Z-stack series of pictures taken in the inner side of the apical hook using the Leica software LAS AF 3.1. The same region of interest (ROI) was defined for each individual seedling. Sample size is given in the figure legends and the data was confirmed in 3 independent experiments. Representative experiments are shown.
Béziat C., Barbez E., Feraru M.I., Lucyshyn D, & Kleine-Vehn J. (2017). Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition. Nature plants, 3, 17105.
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