D-glucose, D-fructose, and D-allulose were analyzed with a Thermo Scientific™ Dionex™ ICS-6000 ion chromatography system (HPIC) equipped with a Dionex CarboPac™ PA20 BioLC™ 3 × 150 mm analytical column. The mobile phase A was pure water, and B was water with 200 mM NaOH. The gradient (B%) was as follows: 0–15 min 10%, 15.1–25 min 100%, 25.1–35 min 10%. The flow rate was 0.5 mL/min. The oven and detector temperature was maintained at 30°C. The inject volume was 25 μL.
Dionex ics 6000
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex ICS-6000 is an ion chromatography system designed for high-performance liquid chromatography. It features a modular design and provides precise and reliable ion analysis capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Dionex carbopac pa200, by Thermo Fisher Scientific (1 mentions)
Chromeleon 7, by Thermo Fisher Scientific (1 mentions)
C18 column, by Waters Corporation (1 mentions)
Cellobiose, by Megazyme (1 mentions)
Mannobiose, by Megazyme (1 mentions)
Mannotriose, by Megazyme (1 mentions)
Xylobiose, by Megazyme (1 mentions)
Xylotriose, by Megazyme (1 mentions)
Carbopac pa200, by Thermo Fisher Scientific (1 mentions)
Xylotetraose, by Megazyme (1 mentions)
Xylopentaose, by Megazyme (1 mentions)
Xylohexaose, by Megazyme (1 mentions)
Toc vcph analyzer, by Shimadzu (1 mentions)
Icap 6000, by Thermo Fisher Scientific (1 mentions)
Sodium y zeolite, by Merck Group (1 mentions)
Nitrate standards, by Merck Group (1 mentions)
Ionpac as11, by Thermo Fisher Scientific (1 mentions)
Hq30d, by HACH (1 mentions)
Agilent 5110 icp oes, by Agilent Technologies (1 mentions)
Toc l, by Shimadzu (1 mentions)
13 protocols using dionex ics 6000
1
Carbohydrates Analysis by Ion Chromatography
2
Quantitative Metabolomic Analysis of Glutamine-Depleted Cells
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Using glutamine-free DMEM (11960044, Gibco) supplemented with 500 μM [13C5]-l -glutamic acid (IR-30570, IsoReag, Shanghai Zzbio Co. Ltd.) and 10% FBS, we labeled 786-O cells (transfected with siRNA targeting GLUL or scrambled siRNA) for 24 hours. After washing and metabolites extraction, clear supernatant was collected and analyzed by Dionex ICS-6000 high-performance ion exchange chromatography–MS/MS (HPIC-MS/MS) with the method developed by Shanghai Biotree Biomedical Technology Co. Ltd. (Shanghai, China) (68 (link)). Briefly, cells were washed with ice-cold normal saline and extracted with 500 μl of extract solvent (MeOH/H2O, 3:1, precooled at −40°C). After vortexing for 30 s, the samples were homogenized for 4 min at 35 Hz with a shaking table. The homogenized tissue extract was sonicated in ice water bath for 5 min, followed by incubation at −40°C for 1 hour and centrifugation at 12000 rpm at 4°C for 15 min. The supernatant was collected and analyzed by HPIC-MS/MS. Standard solutions of metabolites were used for calibration of PIC-MS/MS.
3
HPAEC-PAD Quantification of C1-Oxidized Oligosaccharides
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Quantification of C1-oxidized dimers and trimers
(GlcGlc1A and Glc2Glc1A) was performed using high-performance
anion exchange
chromatography with pulsed amperometric detection (HPAEC-PAD), using
a 26 min gradient as described previously.49 (link) HPAEC-PAD was performed with a Dionex ICS6000 (Thermo Fisher Scientific,
Waltham, MA, USA) equipped with a 1 × 250 mm Dionex CarboPac
PA-200 analytical column attached to 1 × 50 mm Dionex CarboPac
PA-200 guard column. The operational flow was at 63 μL/min,
and 4 μL samples were injected. Eluent generator cartridges
were used containing methanesulfonic acid (MSA) and potassium hydroxide
(KOH) to produce potassium methanesulfonate salts (KMSA). To produce
C1-oxidized standards, native cellobiose and cellotriose (Megazyme,
Bray, Ireland) were mixed to a final concentration of 0.5 mM and incubated
at 40 °C overnight with 2 μM cellobiose dehydrogenase from Myriococcim thermophilum (MtCDH,
produced in-house50 (link)). Control reactions
without ascorbate were included for all enzyme variants but are omitted
from figures for clarity as significant product formation was never
observed.
(GlcGlc1A and Glc2Glc1A) was performed using high-performance
anion exchange
chromatography with pulsed amperometric detection (HPAEC-PAD), using
a 26 min gradient as described previously.49 (link) HPAEC-PAD was performed with a Dionex ICS6000 (Thermo Fisher Scientific,
Waltham, MA, USA) equipped with a 1 × 250 mm Dionex CarboPac
PA-200 analytical column attached to 1 × 50 mm Dionex CarboPac
PA-200 guard column. The operational flow was at 63 μL/min,
and 4 μL samples were injected. Eluent generator cartridges
were used containing methanesulfonic acid (MSA) and potassium hydroxide
(KOH) to produce potassium methanesulfonate salts (KMSA). To produce
C1-oxidized standards, native cellobiose and cellotriose (Megazyme,
Bray, Ireland) were mixed to a final concentration of 0.5 mM and incubated
at 40 °C overnight with 2 μM cellobiose dehydrogenase from Myriococcim thermophilum (MtCDH,
produced in-house50 (link)). Control reactions
without ascorbate were included for all enzyme variants but are omitted
from figures for clarity as significant product formation was never
observed.
4
HPLC Analysis Using Dionex Instruments
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HPLC was performed using a Dionex ICS-6000 instrument equipped with photodiode array detector (PDA-1; Thermo Scientific), autosampler (AS-AP; Thermo Scientific), and fraction collector (ASX-280-FC; Thermo Scientific) and employing a C18 column (3.5 µm, 250 × 4.6 mm and 5 µm, 250 × 10 mm) from Waters, Ltd. Chromeleon 7 (Thermo Scientific) was the software package used for instrument control and analysis. All injections and subsequent runs were monitored at both 254- and 450-nm wavelength.
5
Batch Adsorption of Cadmium in Water
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The adsorption studies were conducted in batch mode by mixing a certain volume of 200 mg/L Cd2+ solution with different water matrices to a final volume of 50 mL and concentration of 20 mg/L. Ultrapure water (Puris MR-RO1600, Mirae ST, Anyang, Korea), tap water (from Nazarbayev University labs) and river water (sampled from 43°15’4.0”N 76°51’50.7”E, Bolshaya Almatinka, Almaty, Kazakhstan) were used without any further purification. The cryogels were comparedwith commercial ion exchange materials, namely an H⁺-form ion exchange resin (Merck, Darmstadt, Germany) and sodium Y synthetic zeolite (Sigma Aldrich, Darmstadt, Germany). A mass of 0.1 g of each adsorbent was mixed with 50 mL of 20 mg/L Cd2+ solutions at room temperature. Samples were withdrawn at 30 min, 2 h and 24 h and the residual Cd2+ was measured by a Dionex ICS 6000 ion chromatography system (Thermo Scientific, Waltham, MA, USA) after filtering through 0.45 µm hydrophilic filter and dilution. The experiments were done in duplicate and average values are reported. The average standard deviation was less than 3%.
6
Monosaccharide Analysis of EPS
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After the EPS samples were acid hydrolyzed, their monosaccharide composition was analyzed by high performance anion exchange chromatography (HPAEC) (DIONEX ICS-6000, Thermo Fisher Scientific, Waltham, MA, USA) equipped with Dionex™ CarbopacTM PA-20 anion exchange chromatography column (3 × 150 nm) and electrochemical detector. NaOH (5 mmol) was used as the mobile phase with a flow rate of 0.4 mL/min at 30 °C [17 (link)]. Established calibration curves of mannose, rhamnose, glucose, galactose, and arabinose were used for quantitative analysis [18 (link)].
7
Oligosaccharide Composition Analysis via HPAEC
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The composition of specific oligosaccharides contained in the HOS preparations (modified minimal salts media) was determined using a Dionex™ ICS-6000 high-performance anion exchange chromatography (HPAEC) system (Thermo Fisher Scientific, Madison, WI) fitted with a CarboPac™ PA200 analytical column (250 mm × 3 mm) and a corresponding microbore guard column (50 mm × 3 mm). The pulsed amperometric detection (PAD) system of ICS-6000 had a AgCl reference electrode and a gold working electrode. The mobile phases were composed of solvent A: 100 mM NaOH, and solvent B: 100 mM NaOH mixed with 320 mM sodium acetate. A gradient elution method was used as follows; hold 100% solvent A for 15 min, then ramp to 50% solvent B at a linear rate for 40 min, afterward increase solvent B to 100% in 1 min and hold constant for 4 min; finally, return the mobile phase to 100% solvent A in 1 min. The eluent flow rate was 0.5 ml/min, injection volume was 10 μl, and the column temperature was 35°C. Pure standards (>95%) of cellobiose, xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, mannobiose, mannotriose, arabinobiose, and arabinotriose, purchased from Megazyme (Wicklow, Ireland), were used to calibrate the instrument.
8
Elemental and Organic Analysis of Liquid Samples
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Elemental analysis on the liquid samples diluted with aqueous nitric acid (HNO3, Merck, superpure grade) was conducted by inductively coupled plasma optical emission spectrometry (ICP-OES) using a Thermo Fisher ICAP 6000 instrument. The calibration was performed from standards certified by the external calibration method (VWR). Total carbon (TC) was also quantified and used to quantity organic carbon (TOC) with a Shimadzu TOC V CPH analyzer equipped with a Shimadzu NDIR detector. Dissolved anions were quantified by ion chromatography with a Thermo Scientific Dionex ICS 6000 ion chromatograph equipped with an eluent generator (potassium hydroxide, KOH) Thermo Scientific Dionex EGC III, a precolumn Thermo Scientific Dionex Ionpac AG11-HC, a Thermo Scientific Dionex Ionpac AS11 column, and a conductivity meter.
9
Hg2+ Adsorption in Water Matrices
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The adsorption studies were conducted in batch mode by dissolving 10 mg/L Hg 2+ in different water matrices by using 200 mg/L Hg(NO3)2 stock solution. Ultra-pure water (Puris MR-RO1600 (Mirae ST, South Korea)), tap water (tap water from Nazarbayev University labs), river water (43°15'04.0"N 76°51'50.7"E, Bolshaya Almatinka, Almaty, Kazakhstan) and natural seawater (38°20'54.0"N 0°28'23.5"W, Alicante, Spain) were used without any further purification. For the removal experiments commercially available activated carbon (GUNT, Germany), ion-exchange resin (strongly acidic, H⁺-form, Merck), synthetic zeolite (sodium Y zeolite, Sigma Aldrich) and synthesized AAC and SAC cryogels were used. A mass of 0.1 g of each material was mixed with 50 mL of 10 mg/L Hg(NO3)2 solutions made by using different water matrix at room temperature. Samples were withdrawn at 10 min, 1 h, 4 h and 24 h and the residual mercury was measured by a mercury analyzer. Also, the cations and anions in the solutions were measured by a Dionex ICS 6000 ion chromatography system (Thermo Scientific, USA) after filtering through 0.45 µm hydrophilic filter and dilution. The experiments were done in duplicate and average values are reported. The average standard deviation was 3%.
10
Quantification of Nitrate Levels
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The nitrate content in the cultures were determined in the supernatant after centrifugation of the samples. The exact method was published elsewhere [36 (link)]. A Dionex ICS-6000 ion chromatography system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an IonPac AS11 and guard column (Thermo Fisher Scientific, Waltham, MA, USA) was used. Detection was done via a conductivity detector unit (Thermo Fisher Scientific, Waltham, MA, USA). Quantification was possible via calibration of nitrate standards (Sigma-Aldrich, St. Louis, MO, USA).
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