Bafilomycin a1 hy 100558

Manufactured by MedChemExpress

Sourced in United States

Bafilomycin A1 (HY-100558) is a laboratory compound produced by MedChemExpress. It is a macrolide antibiotic that functions as a selective and potent inhibitor of vacuolar-type H+-ATPase (V-ATPase).

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Lab products found in correlation

Rapamycin hy 10219, by MedChemExpress (1 mentions) Chloroquine hy 17589a, by MedChemExpress (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Penicillin, by Yeasen (1 mentions) Streptomycin, by Yeasen (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Lc3b sc 376404, by Santa Cruz Biotechnology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) P mtor ser2448, by Cell Signaling Technology (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions) Concanavalin a (cona), by Merck Group (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) β actin, by ZSGB-BIO (1 mentions) Ciclopirox olamine 1134030, by Merck Group (1 mentions) Cq hy 17589a, by MedChemExpress (1 mentions) E64d e8640, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Sorafenib hy 10201, by MedChemExpress (1 mentions)

Spelling variants of this product

Bafilomycin A1 (44 citations) HY-100558 (6 citations)

5 protocols using bafilomycin a1 hy 100558

Establishing Radioresistant Glioblastoma Cell Lines

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Human glioblastoma cell lines of U251 (TCHu 58) and T98G (CRL 1690) were purchased from Cell Bank of Chinese Academy of Science. The stable radioresistant U251R cell line was developed from its parental cell line U251 by exposing it with 2 Gy X-ray/day for 30 fractions (5 fractions/weekly in general) with a total dose of 60 Gy. Human astrocyte HA1800 cells (C1244) and human microglia HMC3 cells (C1026) were kindly provided by Shanghai WHELAB Bioscience Limited. U251, U251R and HA1800 cells were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, C11995500BT), while T98G and HMC3 cells were cultured in Minimum Essential medium (Thermo Fisher Scientific, C12571500BT). The above media contained 10% fetal bovine serum, 100 units/mL penicillin and 100 mg/mL streptomycin (Yeasen Biotechnology, 40130ES76). Cells were incubated in a 5% CO₂-humidified incubator at 37°C. Cells in log-phase were irradiated with a dose rate of 0.883 Gy/min X-ray by X-Rad320 irradiator (PXI Inc., Santa Rosa, CA, USA). For regulation of autophagic activity, rapamycin (HY-10219), bafilomycin A₁ (HY-100558) and chloroquine (HY-17589A) from MedChemExpress were employed.

Zheng W., Chen Q., Liu H., Zeng L., Zhou Y., Liu X., Bai Y., Zhang J., Pan Y, & Shao C. (2022). SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes. Autophagy, 19(3), 839-857.

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Autophagy and Apoptosis Modulation Protocol

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ConA was purchased from Sigma–Aldrich (St. Louis, MO, USA). MP (HY-B0260, analytical standard >99.0%) and bafilomycin A1 (HY-100558, analytical standard >99.0%) were obtained from MedChemExpress (NJ, USA). Antibodies p62/SQSTM1 (cat# 5114), Beclin-1 (cat# 3495), p-mTOR (Ser 2448, cat# 5536), mTOR (cat# 2983), p-Akt (Ser 473, cat# 4060), Akt (cat# 4691), Bax (cat# 2772), Bcl-2 (cat# 3498), and cleaved caspase-3 (cat# 9664) were purchased from Cell Signaling Technology (Danvers, MA, USA). LC3B (sc-376404) was purchased from Santa Cruz Biotechnology (Europe), and β-actin was purchased from ZSGB-BIO (Beijing, China). Monodansylcadaverine (MDC, G0170) was purchased from Solarbio (Beijing, China). The tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining kit was purchased from Beyotime (Shanghai, China). Other reagents were of high analytical grade and were commercially available unless otherwise specified.

Fan X., Men R., Wang H., Shen M., Wang T., Ye T., Luo X, & Yang L. (2019). Methylprednisolone Decreases Mitochondria-Mediated Apoptosis and Autophagy Dysfunction in Hepatocytes of Experimental Autoimmune Hepatitis Model via the Akt/mTOR Signaling. Frontiers in Pharmacology, 10, 1189.

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Autophagy Pathway Modulation Assay

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The following primary antibodies were used: LC3 (Sigma Aldrich Co, St Louis, MO, USA), p62 (Cell Signaling Technology, Boston, MA, USA), β-actin (Cell Signaling Technology).
Ciclopirox olamine (1134030), E64D (E8640) and Pep A (P5318) were purchased from Sigma. Bafilomycin A1 (HY-100558) and CQ (HY-17589A) were purchased from MedChemExpress (Trenton, NJ, USA).

Wan X., Xiang J., Fan H., Jiang Y., Lu Y., Zhang C., Zhang Y., Chen Q, & Lei Y. (2023). Ciclopirox Olamine Induces Proliferation Inhibition and Protective Autophagy in Hepatocellular Carcinoma. Pharmaceuticals, 16(1), 113.

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Small Molecule-Induced Cell Death

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Erastin (E7781), Ferrostatin-1 (SML0583), Deferoxamine (D9533), and Necrosulfonamide (480073) were obtained from Merck KGaA (Darmstadt, Germany). Bafilomycin A1 (HY-100558), sorafenib (HY-10201), and ZVAD-FMK (HY-16658B) were obtained from Med Chem Express (MCE, Shanghai, China).

Sun J., Liu Q., Jiang Y., Cai Z., Liu H, & Zuo H. (2023). Engineered small extracellular vesicles loaded with miR-654-5p promote ferroptosis by targeting HSPB1 to alleviate sorafenib resistance in hepatocellular carcinoma. Cell Death Discovery, 9, 362.

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Propagation and Treatment of PEDV

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Dulbecco's Modified Eagle Medium (DMEM; Invitrogen, Carlsbad, CA, United States) containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, United States) and 1% penicillin–streptomycin (NCM Biotech, Newport, RI, United States) was used to culture African green monkey kidney cells (Vero). IPEC-J2 cells (provided by Dr. Li Wang, Institute of Animal Science, Guangdong Academy of Agricultural Sciences China) were cultured in DMEM/F-12 (Gibco; Invitrogen, Carlsbad, CA, United States) containing 10% FBS and 1% penicillin–streptomycin. Both cell lines were cultured at 37°C in a humidified atmosphere with 5% CO₂. The PEDV strain GD/HZ/2016 (GenBank Accession: OP191700.1) was isolated, identified, and stored in our laboratory at the Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China. The GD/HZ/2016 strain was propagated in Vero cells in DMEM containing trypsin. Fangchinoline (HY-N1372A), wortmannin (SL-2052), chloroquine (HY-17589A), and bafilomycin A1 (HY-100558) were purchased from MedChemExpress (Monmouth Junction, NJ, United States).

Zhang W., Shen H., Wang M., Fan X., Wang S., Wuri N., Zhang B., He H., Zhang C., Liu Z., Liao M., Zhang J., Li Y, & Zhang J. (2023). Fangchinoline inhibits the PEDV replication in intestinal epithelial cells via autophagic flux suppression. Frontiers in Microbiology, 14, 1164851.

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