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Cd3e pe

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CD3e-PE is a fluorescently labeled antibody used to detect and analyze T cells in flow cytometry applications. It binds specifically to the CD3 epsilon chain, which is a component of the T cell receptor complex expressed on the surface of T lymphocytes.

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Lab products found in correlation

Ly6g apc, by BD (2 mentions) Cd80 fitc, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Cd86 apc, by Thermo Fisher Scientific (1 mentions) Cpg 1826, by Sangon (1 mentions) Cd8a fitc, by Thermo Fisher Scientific (1 mentions) Invivomab anti mouse pd 1, by BioXCell (1 mentions) Cd11c pe, by Thermo Fisher Scientific (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Ifn γ, by R&D Systems (1 mentions) Optilyse c, by Beckman Coulter (1 mentions) Cd11b alexafluor488, by Thermo Fisher Scientific (1 mentions) Cd19 efluor450, by Thermo Fisher Scientific (1 mentions) Cd8a apc efluor 780, by Thermo Fisher Scientific (1 mentions) F4 80 pe cy7, by Thermo Fisher Scientific (1 mentions) Kaluza analysis software, by Beckman Coulter (1 mentions) Cd4 percp, by BD (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Cd86 pe, by Thermo Fisher Scientific (1 mentions) Cd8 pe, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PE-anti-mouse CD3e mAb PE-labeled anti-CD3e (clone 145-2C11) CD3e PE-Cy7 Anti-mouse CD3e-FITC + anti-mouse MHC-II-PE CD3e (145-2C11)-PE Anti-mouse CD3e-FITC + anti-mouse MHC-I-PE Anti-mouse CD3e-PE Anti-CD3e PE-Cy7 Anti-mouse CD3e-PE-cy5 Rat anti-mouse CD3e PE-Cy7

5 protocols using cd3e pe

1

Dendritic Cell Activation Assay

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PRT, OVA, propidium iodide (PI) and lipopolysaccharides (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). CpG 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) was bought from Sangon (Shanghai, China). Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) from mouse were bought from Novus Biologicals (Colorado, USA). The antibodies such as CD11c-PE, CD86-APC, CD80-FITC, CD3e-PE, CD8a-FITC and OVA257-264 (SIINFEKL) peptide bound to H-2 Kb APC for flow cytometry (FCM) detection, and the biotinylated OVA257-264 (SIINFEKL) peptide bound to H-2 Kb monoclonal antibody for immunofluorescence, these antibodies were all bought from eBioscience (CA, USA). The ELISA kits for mouse interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) were bought from R&D Systems (Minneapolis, MA, USA). The aPD-1 (InVivoMab anti-mouse PD-1) was purchased from BioXcell (New Hampshire, USA). Avidin-FITC was bought from Boster Bio-Tech (Wuhan, China).
Jiang M., Zhao L., Cui X., Wu X., Zhang Y., Guan X., Ma J, & Zhang W. (2021). Cooperating minimalist nanovaccine with PD-1 blockade for effective and feasible cancer immunotherapy. Journal of Advanced Research, 35, 49-60.
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2

Immune Cell Profiling by Flow Cytometry

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Blood cells from whole blood were incubated with fluorochrome-conjugated antibodies for 30 min at room temperature. After incubation, samples were washed with PBS containing 5% FBS, after which Optilyse C (Beckman Coulter, A11895) was added to lyse erythrocytes. Antibodies against the following proteins were used: CD3e-PE (Clone 145-2C11; 12-0031-82), CD8a-APC-eFluor780 (Clone: 53-6.7; 47-0081-82), CD11b-AlexaFluor488 (Clone: M1/70; 53-0112-82), CD19-eFluor 450 (Clone: 1D3; 48-0193-82) and F4/80-PE-Cy7 (Clone: BM8; 25-4801-82) purchased from eBioscience. From BD Bioscience CD4-PerCP (Clone RM4-5; 553,052), and Ly6G-APC (Clone 1A8; 560599) were used. The Gallios Flow Cytometer (Beckman Coulter) was used to measure cell fluorescence and all analyses were performed with Kaluza Analysis Software (Beckman Coulter, version 1.3). Results are expressed as the number of immune cells per milliliter. To determine this concentration, the number of cells determined in a fixed volume of blood (20 μL at baseline and 50 μL at termination) was multiplied by 50 and 20, respectively, to estimate the number of cells in 1 mL. The gating strategy can be found in the Supplementary Figure S2.
Kilgallen A.B., van den Akker F., Feyen D.A., Crnko S., Snijders Blok C.J., Gremmels H., du Pré B.C., Reijers R., Doevendans P.A., de Jager S.C., Sluijter J.P., Sampaio-Pinto V, & van Laake L.W. (2022). Circadian Dependence of the Acute Immune Response to Myocardial Infarction. Frontiers in Pharmacology, 13, 869512.
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3

Multiparametric Flow Cytometry Analysis

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Splenocytes were resuspended in 100 µL of PBS, and cells were stained with anti-mouse CD11c-fluorescein isothiocyanate (FITC), CD83-phycoerythrin (PE), CD86-PE, MHC-I-PE, MHC-II-PE, CD3e-FITC, CD4-PE, CD3e-PE, and CD8-PE (eBioscience, San Diego, CA, USA) for 40 min at 4°C in the dark. A flow cytometer (Beckman Coulter Inc., Brea, CA, USA) was used for cell fluorescence analysis. All samples were stained using the above strategy and subjected to flow cytometric analysis. All labeled cells were analyzed on a FACS Aria III flow cytometer (BD Biosciences), and data were analyzed using CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA).
Zhang Y., Li S., Chu H., Li J., Lu S, & Zheng B. (2023). A novel mRNA vaccine, TGGT1_278620 mRNA-LNP, prolongs the survival time in BALB/c mice with acute toxoplasmosis. Microbiology Spectrum, 12(1), e02866-23.
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4

Flow Cytometry Analysis of Immune Cells

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Immune cells were analyzed by flow cytometry, as previously described. To prevent nonspecific binding to the Fcγ receptor, single-cell suspensions were first incubated with an anti-CD16/32 antibody. Fixable viability stain 510 (564406, BD Biosciences) and the following antibodies were used for flow cytometry: CD45-APC-Cy7 (557659, BD Biosciences), CD45-PE (553081, BD Biosciences), CD11b–fluorescein isothiocyanate (FITC; 557396, BD Biosciences), CD11b-PE-Cy7 (552850, BD Biosciences), CD11b-PE (553311, BD Biosciences), CD64-APC (558539, BD Biosciences), Ly6C-PE-Cy7 (560593, BD Biosciences), Ly6C-APC (Invitrogen, 17-5932-82), Ly6G-APC (560599, BD Biosciences), Ly6G-FITC (551460, BD Biosciences), F4/80-BV421 (565411, BD Biosciences), CD3e-BV421(564008, BD Biosciences), CD3e-PE (12-0031-81, eBioscience), CD4-APC(553051, BD Biosciences), CD8a-PE (553033, BD Biosciences), CD8a-FITC (553031, BD Biosciences), MHCII-BV421 (743870, BD Biosciences), CCR2-PE (FAB5538P, R&D Systems), CD86-PE (553692, BD Biosciences), CD206-APC (565250, BD Biosciences), and CD31-PE (553373, BD Biosciences). Flow cytometry and cell sorting were performed using a FACSAria flow cytometer (BD Biosciences). Data are expressed as the absolute number of cells per milligram of tissue and were analyzed using the FlowJo software.
Bai P.Y., Chen S.Q., Jia D.L., Pan L.H., Liu C.B., Liu J., Luo W., Yang Y., Sun M.Y., Wan N.F., Rong W.W., Sun A.J, & Ge J.B. (2022). Environmental eustress improves postinfarction cardiac repair via enhancing cardiac macrophage survival. Science Advances, 8(17), eabm3436.
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5

Multiparameter Flow Cytometry Panel

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PD-1 FITC (1:300; J43; eBioscience), NK1.1 PE (1:300; PK136; eBioscience), CD3e PE-Dazzle594 (1:300; 145-2C11; BioLegend), CD3e PE (1:200; 145-2C11; eBioscience), FoxP3 PerCP-Cy5.5 (1:200; FJK-16s; eBioscience), CD8a PE-Cy7 (1:2000; 53-6.7; eBioscience), CD8a APC (1:600; 53-6.7; eBioscience), CD8a PerCP-Cy5.5 (1:400; 53-6.7; eBioscience), TCRb AF700 (1:100; H57-597; BioLegend), CD45 BV785 (1:1000; 30-F11; BioLegend), CD4 BV711 (1:400; GK1.5; BioLegend), CD19 BV650 (1:400; 6D5; BioLegend), CD19 PE (1:500; 1D3; eBioscience), CD11b BV605 (1:1000; M1/70; BioLegend), CD11b BV510 (1:400; M1/70; BioLegend), CD11c BV605 (1:400; N418; BioLegend), Siglec-F PE (1:300; E50-2440; BD Biosciences), F4/80 APC (1:200; BM8; BioLegend), Ly-6G AF 700 (1:300; 1A8; BioLegend), MHC II BV650 (1:4000; M5/114.15.2; BioLegend), CD64 BV421 (1:200; X54-5/7.1; BioLegend), TNF FITC (1:300; MP6-XT22; BioLegend), IFNg PE-Cy7 (1: 1000; XMG1.2; BD Biosciences), Eomes PE (1:200; W17001A; BioLegend), Ki-67 BV 650 (1:200, 11F6; Biolegend), GzmB FITC (1:100; GB11; BioLegend). Gating strategy is depicted in Supplementary Fig. 6d.
Frey N., Tortola L., Egli D., Janjuha S., Rothgangl T., Marquart K.F., Ampenberger F., Kopf M, & Schwank G. (2022). Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing. Nature Communications, 13, 1804.
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