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Micro2.5 gradient system

Manufactured by Bruker
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The Bruker Micro2.5 gradient system is a compact, high-performance gradient system designed for small-bore magnetic resonance imaging (MRI) applications. The system provides reliable and consistent gradient performance, enabling precise spatial encoding and high-quality imaging.

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Lab products found in correlation

Fluorinert fc 77, by 3M (1 mentions) Fomblin perfluoro polyether, by Solvay (1 mentions) 11.7 t vertical bore scanner, by Bruker (1 mentions) Fomblin, by Solvay (1 mentions)

6 protocols using micro2.5 gradient system

1

MRI Imaging of Tissue Samples

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The tissue samples were removed from the storing solution and cut to a suitable sample size. The specimens were immersed into a 20-mm-NMR-tube with a perfluorinated fluid (Fluorinert™ FC-77, 3M Belgium NV/SA, Zwijndrecht, Belgium) which prevents dehydration and contributes no proton signal during MRI measurements.
MR imaging was performed using a 7-T Bruker Avance nonclinical NMR spectrometer with a vertical-bore magnet (300 MHz Larmor frequency for protons, Bruker BioSpin, Rheinstetten, Germany) using a linear polarized birdcage radiofrequency coil of 20 mm inner diameter and a Bruker Micro 2.5 gradient system generating a maximum magnetic field gradient strength of up to 1 T/m on three axes. The experimental parameters used for imaging are summarized in Table 2.
Elschner C., Korn P., Hauptstock M., Schulz M.C., Range U., Jünger D, & Scheler U. (2017). Assessing agreement between preclinical magnetic resonance imaging and histology: An evaluation of their image qualities and quantitative results. PLoS ONE, 12(6), e0179249.
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2

Testicular MRI Imaging Protocol

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Testes of mice with different genotypes from the same litter were fixed with 4% formaldehyde, soaked in PBS overnight before MRI analysis. All MRI experiments were using a 14 Tesla Bruker Ascend 600WB vertical bore magnet, equipped with a MicWB40 micro-imaging probe in combination with a Micro2.5 gradient system. Images were obtained using Paravision 6.0.1 software. Excised testis samples were bedded on top of 1% agarose gel in a 10mm diameter glass tube, and a quadrature coil with an inner diameter of 25mm was used to transmit/receive the MR signals. Fast Low Angle Shot (FLASH) pulse sequence with gradient echo was used to acquire 2D images. Susceptibility weighted images (SWI) were reconstructed to obtain suitable contrast of blood vessels versus seminiferous tubules.
Bao Q., Morshedi A., Wang F., Bhargy S., Pervushin K., Yu W.P, & Dröge P. (2017). Utf1 contributes to intergenerational epigenetic inheritance of pluripotency. Scientific Reports, 7, 14612.
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3

High-Field MRA of Vascular System

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The magnetic resonance angiography experiments were performed on a 9.4 T vertical 89-mm-bore system (Bruker BioSpin, Rheinstetten, Germany) equipped with a Bruker Micro 2.5 gradient system and a transmit/receive birdcage radio frequency coil with an inner diameter of 30 mm. During data collection, animals were anesthetized with 2–3% solution of sevoflurane. Body temperature and respiration rate were monitored using ECG/respiratory unit (SA Instruments, Inc., Stony Brook, NY, USA). Images of vascular system were acquired using a multi-slice 2D TOF (time of flight) flow compensated sequence using the following parameters: TE = 3.1 ms, TR = 12 ms, flip angle = 80°, field of view 2 × 3cm2, matrix size 200 × 300, slice thickness 0.3 mm, inter-slice distance 0.2 mm, and number of signal averages 3.
Pilny E., Smolarczyk R., Jarosz-Biej M., Hadyk A., Skorupa A., Ciszek M., Krakowczyk Ł., Kułach N., Gillner D., Sokół M., Szala S, & Cichoń T. (2019). Human ADSC xenograft through IL-6 secretion activates M2 macrophages responsible for the repair of damaged muscle tissue. Stem Cell Research & Therapy, 10, 93.
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4

Ex Vivo dMRI of Mouse Brains

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Ex vivo dMRI of the same adult and P11 mouse brains was performed on a vertical 17.6 Tesla NMR spectrometer (Bruker Biospin, Billerica, MA, USA) with a Micro2.5 gradient system (maximum gradient strength = 1500 mT/m) and a 15 mm diameter transceiver volume coil. During MRI, the specimens were immersed in fomblin (Fomblin Perfluoropolyether, Solvay Solexis, Thorofare, NJ, USA) for susceptibility matching and to prevent dehydration. The temperature of the specimens was maintained at 37 °C via the spectrometer’s temperature control system. The same imaging protocol as the in vivo MRI was used, except that a higher b-value (800 s/mm2) was used to compensate for the reduced ADC in the ex vivo brains. The SNR measured in the cortex were greater than 70 for both OGSE and PGSE experiments.
Wu D., Martin L.J., Northington F.J, & Zhang J. (2014). Oscillating gradient diffusion MRI reveals unique microstructural information in normal and hypoxia-ischemia injured mouse brains. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 72(5), 1366-1374.
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5

High-Resolution 3D Diffusion MRI of Brains

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The brains were scanned on an 11.7 T NMR spectrometer (Bruker BioSpin, Billerica, MA, United States), with a Micro2.5 gradient system (maximum gradient strength = 1,000 mT/m). A 20-mm-diameter birdcage volume coil was used for radiofrequency transmission and signal reception. Diffusion data were acquired using a 3D diffusion-weighted gradient- and spin-echo (DW-GRASE) sequence (Aggarwal et al., 2010 (link)) with TR/TE = 800/33 ms, rare-factor/EPI factor = 4/3, bandwidth = 100 kHz, number of averages = 2, FOV = 22.8 mm × 16.8 mm × 11.7 mm, matrix size (read × phase × phase 2) = 152 × 112 × 78, acquired spatial resolution = 150 μm3 isotropic (zero-filling interpolation to 0.075 mm3 isotropic), number of uniformly distributed diffusion directions = 30 for each b-value of 3,000 and 6,000 s/mm2, number of minimally diffusion-weighted images = 3, diffusion gradient duration (δ)/separation (Δ) = 5/12 ms, total acquisition time ∼21 h.
Chary K., Narvaez O., Salo R.A., San Martín Molina I., Tohka J., Aggarwal M., Gröhn O, & Sierra A. (2021). Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury. Frontiers in Neuroscience, 15, 746214.
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6

High-Resolution 3D MRI of Mouse Brains

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The specimens were transferred to PBS for five days before MRI, and placed in 20-mm diameter tubes that were filled with Fomblin (Solvay Inc., Princeton, NJ, USA), a proton-free perfluoropolyether, to prevent dehydration and for susceptibility matching at the tissue-air interface. MRI experiments were performed on an 11.7 T vertical-bore scanner (Bruker Biospin, Billerica, MA, USA) equipped with an actively-shielded Micro2.5 gradient system with a maximum gradient strength of 1500 mT/m. A 20-mm-diameter birdcage volume coil was used as the radiofrequency transceiver. The mouse brains were scanned with the dorsal-ventral axis of the skull oriented along the main magnetic field direction. A 3D multi-echo gradient echo (MGE) sequence with fast flyback for monopolar readout gradients was used for image acquisition. The imaging parameters were as follows: flip angle = 30°, pulse repetition time (TR) = 100 ms, first echo time (TE1) = 4 ms, 8 echoes with inter-echo spacing = 3.8 ms, 4 signal averages, and receiver bandwidth = 70 kHz. The 3D multi-echo data were acquired at an isotropic spatial resolution of 70 μm with a scan time of 3 h 45 min. The imaging field-of-view and acquisition matrix size were 12 mm × 9.2 mm × 17.6 mm and 170 × 132 × 252, respectively.
Joshi J., Yao M., Kakazu A., Ouyang Y., Duan W, & Aggarwal M. (2024). Distinguishing microgliosis and tau deposition in the mouse brain using paramagnetic and diamagnetic susceptibility source separation. bioRxiv.
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