Tumors were fixed in 10% neutral buffered formalin. Samples were then transferred to 75% ethanol and embedded in paraffin. Histologic sections were stained with hematoxylin and eosin or processed for immunohistochemical analysis. Tissue sections used for immunohistochemical analysis were deparaffinized and then rehydrated following xylene and alcohol series. Antigen retrieval was performed in 100 mM sodium citrate (pH 6.0), and endogenous peroxidase was blocked with 1% hydrogen peroxide. Then, tissue sections were incubated with primary antibodies for p-Akt (Epitomics, Burlingame, CA); p-Erk and p-Smad2 (Cell Signaling Technology, Danvers, MA); CD45, Gr-1, F4/80 and Nk1 (BioLegend, San Diego, CA); CD3, α–SMA and Ki67 (Thermo Scientific, Rockford, IL); p16 (Santa Cruz Biotechnology, Santa Cruz, CA). Primary antibodies were detected using the ImmPRESS system (Vector Laboratories, Burlingame, CA). Slides were visualized on a Leica DMLA microscope (Leica Microsystems, Buffalo Grove, IL), and images were captured with a Hamamatsu ORCA-ER camera (Shizuoka, Japan).
P erk and p smad2
Manufactured by Cell Signaling Technology
P-Erk and p-Smad2 are antibodies used in laboratory research. P-Erk is an antibody that detects the phosphorylated form of the extracellular signal-regulated kinase (Erk) protein. p-Smad2 is an antibody that detects the phosphorylated form of the Smad2 protein. These antibodies are used to analyze the activation of cellular signaling pathways.
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Lab products found in correlation
2 protocols using p erk and p smad2
1
Immunohistochemical Analysis of Tumor Samples
2
Immunohistochemical Analysis of Tumor Samples
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Tumors were fixed in 10% neutral buffered formalin. Samples were then transferred to 75% ethanol and embedded in paraffin. Histologic sections were stained with hematoxylin and eosin or processed for immunohistochemical analysis. Tissue sections used for immunohistochemical analysis were deparaffinized and then rehydrated following xylene and alcohol series. Antigen retrieval was performed in 100 mM sodium citrate (pH 6.0), and endogenous peroxidase was blocked with 1% hydrogen peroxide. Then, tissue sections were incubated with primary antibodies for p-Akt (Epitomics, Burlingame, CA); p-Erk and p-Smad2 (Cell Signaling Technology, Danvers, MA); CD45, Gr-1, F4/80 and Nk1 (BioLegend, San Diego, CA); CD3, α–SMA and Ki67 (Thermo Scientific, Rockford, IL); p16 (Santa Cruz Biotechnology, Santa Cruz, CA). Primary antibodies were detected using the ImmPRESS system (Vector Laboratories, Burlingame, CA). Slides were visualized on a Leica DMLA microscope (Leica Microsystems, Buffalo Grove, IL), and images were captured with a Hamamatsu ORCA-ER camera (Shizuoka, Japan).
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