Scramble mirna probes

Manufactured by Qiagen

Sourced in Denmark

Scramble-miRNA probes are a type of molecular probe designed for use in microRNA (miRNA) analysis. They serve as a control to help assess the specificity and background levels in miRNA detection and quantification experiments.

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Lab products found in correlation

Yeast rna, by Thermo Fisher Scientific (1 mentions) Diaminobenzidine dab, by ZSGB-BIO (1 mentions)

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Scramble probes (2 citations) MiRNA probes (2 citations)

2 protocols using scramble mirna probes

In-Situ Hybridization of miRNA Probes

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Hybrid hsa-miR-96, hsa-miR-217, U6 and scramble-miRNA probes were purchased from Exiqon (Vedbaek, Denmark). The hsa-miR-217, hsa-miR-96, U6, and scramble-miRNA probes were labeled with 5' digoxigenin (5'DIG). Yeast RNA was purchased from Ambion (United States). DEPC was purchased from Invitrogen (California, United States). Anti-digoxigenin alkaline phosphatase-labeled secondary anti-DIG-AP Fab and NBT/BCIP staining kit were purchased from Roche (Mannheim, Germany). The sequences of the probes are shown in Table 1.

Chang X., Yu C., Li J., Yu S, & Chen J. (2017). hsa-miR-96 and hsa-miR-217 Expression Down-Regulates with Increasing Dysplasia in Pancreatic Intraepithelial Neoplasias and Intraductal Papillary Mucinous Neoplasms. International Journal of Medical Sciences, 14(5), 412-418.

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Microwave Radiation's Impact on miR-30a

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At 7 and 14 days after microwave radiation, rat brains were collected and fixed in 10% buffered formalin solution, embedded in paraffin and cut into 3-μm-thick sections. Then, miR-30a expression was assessed by in situ hybridization (ISH). Briefly, the sections were hybridized with miR-30a, U6, or scramble miRNA probes (Exiqon, Vedbaek, Denmark). After being labeled with anti-DIG (Boster Biological Technology, China) and stained with diaminobenzidine (DAB) (ZSGB-Bio), the expression of miR-30a and U6 was assessed in a blind manner under a light microscope (Leica). To quantify miR-30a expression, Image-Pro Plus 6.0 was used to analyze the IOD, and the expression of miR-30a was normalized to that of U6.

Hao Y., Li W., Wang H., Zhang J., Wang H., Dong J., Yao B., Xu X., Zhao L, & Peng R. (2022). Microwave radiation induces neuronal autophagy through miR-30a-5p/AMPKα2 signal pathway. Bioscience Reports, 42(4), BSR20212584.

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