Log In Sign up
The largest database of trusted experimental protocols

Ab105385

Manufactured by Abcam
Visit Supplier

Ab105385 is a highly specific monoclonal antibody that recognizes the human SMAD1 protein. SMAD1 is a transcription factor that mediates the signaling of bone morphogenetic proteins (BMPs). This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the SMAD1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Glomax luminometer, by Promega (2 mentions) Igg isotype control, by Abcam (2 mentions) Luciferase assay kit, by Promega (2 mentions)

Close spelling variants of this product

Mouse polyclonal anti-KIAA0319L (ab105385), (2 citations)

2 protocols using ab105385

1

Anti-AAVR Antibody Neutralization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type HeLa cells were seeded in 96-well plates at 10,000 cells/well overnight. Anti-AAVR antibody (ab105385) or IgG isotype control (both from Abcam, Cambridge, CA) were incubated with cells (at concentrations ranging from 0.5 to 50 μg/ml in DMEM media) for 1 hr at 4°C. Cells were then infected with AAV2-luciferase at MOI 1,000 vg/cell, and left for 24 hrs at 37°C. A luciferase assay kit (#E1500, Promega, Madison, WI) was used to detect bioluminescence, with measurements being taken on the Promega GLOMAX luminometer. Importantly, the storage buffers of both antibodies did not contain preservatives such as azide that could interfere with the assay. All data presented is representative of two independent experiments.
Pillay S., Meyer N.L., Puschnik A.S., Davulcu O., Diep J., Ishikawa Y., Jae L.T., Wosen J.E., Nagamine C.M., Chapman M.S, & Carette J.E. (2016). An essential receptor for adeno-associated virus infection. Nature, 530(7588), 108-112.
+ Open protocol
+ Expand
2

Anti-AAVR Antibody Neutralization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type HeLa cells were seeded in 96-well plates at 10,000 cells/well overnight. Anti-AAVR antibody (ab105385) or IgG isotype control (both from Abcam, Cambridge, CA) were incubated with cells (at concentrations ranging from 0.5 to 50 μg/ml in DMEM media) for 1 hr at 4°C. Cells were then infected with AAV2-luciferase at MOI 1,000 vg/cell, and left for 24 hrs at 37°C. A luciferase assay kit (#E1500, Promega, Madison, WI) was used to detect bioluminescence, with measurements being taken on the Promega GLOMAX luminometer. Importantly, the storage buffers of both antibodies did not contain preservatives such as azide that could interfere with the assay. All data presented is representative of two independent experiments.
Pillay S., Meyer N.L., Puschnik A.S., Davulcu O., Diep J., Ishikawa Y., Jae L.T., Wosen J.E., Nagamine C.M., Chapman M.S, & Carette J.E. (2016). An essential receptor for adeno-associated virus infection. Nature, 530(7588), 108-112.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!