Intense r detection kit

Manufactured by Leica

The Intense R detection kit is a laboratory equipment product designed for the detection of specific target molecules or compounds. It provides a reliable and sensitive method for identifying the presence of the targeted analyte in a sample. The core function of this kit is to enable accurate and reproducible detection through the use of specialized reagents and protocols.

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Lab products found in correlation

Normal rabbit serum, by Vector Laboratories (2 mentions) Anti col1, by Abcam (1 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions) Texas red, by Agilent Technologies (1 mentions) Anti α sma antibody, by Abcam (1 mentions) Bond autostainer, by Leica (1 mentions) Anti brdu antibody, by Thermo Fisher Scientific (1 mentions) Anti cd3, by BD (1 mentions) Anti f4 80, by Thermo Fisher Scientific (1 mentions) Anti b220, by BD (1 mentions) F4 80, by Leica (1 mentions) Biotinylated secondary antibody, by Vector Laboratories (1 mentions) Bond max autostainer, by Leica (1 mentions)

Spelling variants of this product

Bond Intense R Detection kit (2 citations)

3 protocols using intense r detection kit

Histological Evaluation of Tumor Extracellular Matrix

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Formalin-fixed, paraffin-embedded tumor sections were processed by standard methods for hematoxylin and eosin staining, immunohistochemistry (IHC), and immunofluorescence. HA staining was detected using a modified TNF-stimulated gene 6 protein (TSG-6, 0.25 μg/mL; Halozyme; ref. 35 (link)), followed by either fluorescein-HRP (Vector Labs) and Texas Red (Dako) staining or staining with the Intense R detection kit (Leica Biosystems). Collagen I (ColI) and α-SMA were detected using anti-ColI and anti–α-SMA antibodies, respectively (Abcam), followed by fluorescein-horseradish peroxidase (HRP) and fluorescein isothiocyanate (FITC; Dako) or DAB (Agilent). HA, ColI, and α-SMA were then imaged and quantified as described below.

Li X., Shepard M.H., Cowell J.A., Zhao C., Osgood R.J., Rosengren S., Blouw B., Garrovillo S.A., Pagel M.D., Whatcott C.J., Han H., Von Hoff D.D., Taverna D.M., LaBarre M.J., Maneval D.C, & Thompson C.B. (2018). Parallel Accumulation of Tumor Hyaluronan, Collagen, and Other Drivers of Tumor Progression. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(19), 4798-4807.

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Immunohistochemical Analysis of Mouse Colon

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Mouse colons were fixed in neutral buffered formalin and embedded in paraffin. Sections at 3 μm were prepared and stained with H&E. IHC for CD3, B220 and F4/80 to identify T cells, B cells and macrophages, respectively, was performed on LeicaBiosystems Bond Autostainer. Sections were blocked with 2% normal rabbit serum (Vector Laboratories) for 20 minutes and subsequently incubated for 30 minutes with anti-CD3 (AbDSerotec), anti-B220 (BD Biosciences) or anti-F4/80 (eBiosciences) Ab. Biotinylated secondary antibody (anti-rabbit IgG), diluted 1:100 in 1.5% normal rabbit serum, was applied to the slides for 30 minutes and subsequent detection was achieved with Leica Biosystems’ Intense R detection Kit. IHC for BrdU was performed manually using anti-BrdU antibody (Invitrogen) and the Vectastain Elite Standard ABC kit (Vector Laboratories).

Yoshimura T., McLean M.H., Dzutsev A.K., Yao X., Chen K., Huang J., Gong W., Zhou J., Xiang Y., Badger J., Ohuigin C., Thovarai V., Tessarollo L., Durum S.K., Trinchieri G., Bian X.W, & Wang J.M. (2018). The Anti-microbial Peptide CRAMP Is Essential for Colon Homeostasis by Maintaining Microbiota Balance. Journal of immunology (Baltimore, Md. : 1950), 200(6), 2174-2185.

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Immunohistochemical Analysis of Tumor Xenografts

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Frozen samples of tumor xenografts were submitted to the Pathology/Histotechnology Laboratory (PHL) at NCI-Frederick. Samples were thawed in 10% buffered neutral formalin, paraffin-embedded, and cut into 5-mm sections. Staining was performed using the Bond Max Autostainer from Leica Biosystems (Buffalo Grove, IL). EDTA was used for antigen retrieval and 10% normal rabbit serum (Vector Laboratories) was used as blocking buffer. Sections were incubated with a 1:100 dilution of VEGF or CD31 antibodies (R & D Systems) for 30 min, followed by incubation with a 1:500 dilution of biotinylated secondary antibody (Vector Laboratories) for 30 min. Immune reactivity was visualized with the Intense R Detection Kit (Leica Biosystems). Sections were counterstained with hematoxylin and eosin (H & E). All slides were imaged on an Aperio bright field ScanScope.

Reece K.M., Richardson E.D., Cook K.M., Campbell T.J., Pisle S.T., Holly A.J., Venzon D.J., Liewehr D.J., Chau C.H., Price D.K, & Figg W.D. (2014). Epidithiodiketopiperazines (ETPs) exhibit in vitro antiangiogenic and in vivo antitumor activity by disrupting the HIF-1α/p300 complex in a preclinical model of prostate cancer. Molecular Cancer, 13, 91.

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