Cellrox deep red

Manufactured by Nikon

CellROX Deep Red is a fluorogenic probe designed to measure oxidative stress in live cells. It is a cell-permeant dye that exhibits strong fluorescence upon oxidation by reactive oxygen species (ROS).

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Lab products found in correlation

Cellrox deep red, by Thermo Fisher Scientific (2 mentions) Mitosox, by Nikon (1 mentions) Mitosox, by Thermo Fisher Scientific (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) H2dcfda, by Nikon (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Hoechst 33342, by Nikon (1 mentions)

2 protocols using cellrox deep red

Fluorescent Dye Staining of Fungal Cells

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H₂DCFDA, CellROX DeepRed and Mitosox were purchased from Invitrogen (Molecular Probes, Eugene, OR, USA). Whole mycelia and protoplasts were incubated with dyes in the growth medium for 10–15 min and washed in the medium 3 times according to the manufacturer’s instructions. Samples were observed and photographed under a Nikon fluorescence microscope using a filter with 495 nm excitation and 500–550 nm emission wavelengths (H₂DCFDA and Mitosox staining) or a filter with 510 nm excitation and 580 nm emission wavelengths (CellROX Deep Red staining).

Roze L.V., Laivenieks M., Hong S.Y., Wee J., Wong S.S., Vanos B., Awad D., Ehrlich K.C, & Linz J.E. (2015). Aflatoxin Biosynthesis Is a Novel Source of Reactive Oxygen Species—A Potential Redox Signal to Initiate Resistance to Oxidative Stress?. Toxins, 7(5), 1411-1430.

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Oxidative Stress Imaging in Cultured iNs

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Cultured iNs were incubated with 2.5 μM CellROX Deep Red (Invitrogen) for 30 min and 2 μg/mL Hoechst 33342 (Sigma-Adrich) for 15 min. Treatment of 100 μM H₂O₂ for 90 min was used to be a positive control. Random images were taken on the Nikon Observer of DIC, CellROX Deep Red and Hoechst 33342 fluorescence.

Wu P.C., Fann M.J., Tran T.T., Chen S.C., Devina T., Cheng I.H., Lien C.C., Kao L.S., Wang S.J., Fuh J.L., Tzeng T.T., Huang C.Y., Shiao Y.J, & Wong Y.H. (2019). Assessing the therapeutic potential of Graptopetalum paraguayense on Alzheimer’s disease using patient iPSC-derived neurons. Scientific Reports, 9, 19301.

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