Spleens were collected from mice at days 0, 7, 9, and 11, placed sequentially in a 10, 20, and 30% sucrose gradient overnight, and flash frozen in OCT. Spleens were sectioned in a cryostat and mounted on slides at ~8 um and stored at −80°C. Slides were blocked in 1% BSA diluted in PBS and stained overnight at 4° with B220-AF488 clone RA3–6B2 (ThermoFisher Scientific, Waltham, MA), CD3-AF594 clone 17A2 (Biolegend, San Diego, CA), and GL7-AF647 clone GL7 (BD Biosciences, San Jose, CA). Tissue was covered with ProLong Gold antifade reagent (Thermo Fisher Scientific, Waltham, MA) and imaged using a Leica TCS SP8 confocal microscope. Images were analyzed using Fiji ImageJ software for MAC.
B220 af488 clone ra3 6b2
Manufactured by Thermo Fisher Scientific
The B220-AF488 clone RA3–6B2 is a fluorescently labeled antibody used for the identification and characterization of B cells in flow cytometry applications. The antibody targets the B220 antigen, also known as CD45R, which is expressed on the surface of B lymphocytes.
Automatically generated - may contain errors
2 protocols using b220 af488 clone ra3 6b2
1
Spleen Cryosectioning and Imaging
2
Spleen Cryosectioning and Imaging
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