Approximately 50 μl blood from individual mice was collected every other day from the tail vein into heparinized tubes and centrifuged at 10,000×g for 10 min. Supernatants were transferred to sterile cryopreserved tubes and stored at −80°C prior to analysis using mouse cytokine 20-plex kit and Luminex 200 instrument according to the manufacturer's instructions (Invitrogen). Levels of CC were exported to Excel sheets and subjected to statistical analyses (nonparametric Mann-Whitney U test and one-way ANOVA) using GraphPad Prism version 6.0 (GraphPad Software, Inc.). Differences were deemed significant at p < 0.05.
Mouse cytokine 20 plex kit
Manufactured by Thermo Fisher Scientific
The Mouse cytokine 20-plex kit is a multiplex assay designed to simultaneously measure the concentrations of 20 different mouse cytokines in a single sample. It is a quantitative tool for the detection and analysis of mouse cytokines.
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2 protocols using mouse cytokine 20 plex kit
1
Quantifying Mouse Cytokine Levels
2
Quantifying Mouse Cytokine Levels
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Approximately 50 μl blood from individual mice was collected every other day from the tail vein into heparinized tubes and centrifuged at 10,000×g for 10 min. Supernatants were transferred to sterile cryopreserved tubes and stored at −80°C prior to analysis using mouse cytokine 20-plex kit and Luminex 200 instrument according to the manufacturer's instructions (Invitrogen). Levels of CC were exported to Excel sheets and subjected to statistical analyses (nonparametric Mann-Whitney U test and one-way ANOVA) using GraphPad Prism version 6.0 (GraphPad Software, Inc.). Differences were deemed significant at p < 0.05.
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