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β nicotinamide adenine dinucleotide

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β-nicotinamide adenine dinucleotide is a coenzyme that plays a crucial role in various cellular redox reactions, particularly in the process of energy production within cells. It serves as an essential cofactor for numerous enzymes involved in metabolic pathways.

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Lab products found in correlation

Soluble starch, by Merck Group (5 mentions) Thiamine pyrophosphate, by Merck Group (5 mentions) D glucose, by Carl Roth (5 mentions) K2hpo4, by Carl Roth (5 mentions) Kh2po4, by Carl Roth (5 mentions) Sodium chloride (nacl), by Carl Roth (5 mentions) L glutamine, by Carl Roth (5 mentions) 4 aminobenzoic acid, by Merck Group (5 mentions) Thiamine hcl, by Carl Roth (5 mentions) Vitamin b12, by Merck Group (5 mentions) Fe no3 3, by Merck Group (4 mentions) Proteose peptone no 3, by BD (4 mentions) Dehydrated agar, by BD (3 mentions) Isovitalex, by Carl Roth (2 mentions) L cysteine hcl h20, by Carl Roth (2 mentions) Bacto agar, by BD (2 mentions) L cysteine hcl h2o, by Carl Roth (2 mentions) L cysteine hcl x h2o, by Carl Roth (1 mentions) Bacto proteose peptone no 3, by BD (1 mentions)

5 protocols using β nicotinamide adenine dinucleotide

1

Gonococcal Base Agar Composition

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Gonococcal base agar was made from 10 g/l dehydrated agar (BD Biosciences, Bedford, MA), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/l D-glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL x H2O (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
Cronenberg T., Hennes M., Wielert I, & Maier B. (2021). Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment. PLoS Pathogens, 17(2), e1009251.
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2

Culturing N. gonorrhoeae for Experiments

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N. gonorrrhoeae (Table S1) was grown overnight at 37°C and 5% CO2 on agar plates containing gonococcal base agar (10 g/l Bacto agar (BD Biosciences, Bedford, MA, USA), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO, USA)) and the following supplements: 1 g/l D-Glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL×H20 (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth) and 0.1 mg/l vitamin B12 (Sigma-Aldrich). Before each experiment gonococcal colonies were resuspended in GC-medium.
Gangel H., Hepp C., Müller S., Oldewurtel E.R., Aas F.E., Koomey M, & Maier B. (2014). Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation. PLoS Pathogens, 10(4), e1004043.
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3

Gonococcal Base Agar Preparation

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Media composition, assay preparation, and setup follow [27 (link)]. Gonococcal base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, Massachusetts, United States of America), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L L-cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/LFe(NO3) 3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch. Employed bacterial strains (Table i in S1 Text) are derivatives of strain N. gonorrhoeae MS11.
Hennes M., Bender N., Cronenberg T., Welker A, & Maier B. (2023). Collective polarization dynamics in bacterial colonies signify the occurrence of distinct subpopulations. PLOS Biology, 21(1), e3001960.
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4

Preparation of GC-base Agar Medium

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GC base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, MA), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L l-cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/L Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
Welker A., Hennes M., Bender N., Cronenberg T., Schneider G, & Maier B. (2021). Spatiotemporal dynamics of growth and death within spherical bacterial colonies. Biophysical Journal, 120(16), 3418-3428.
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5

Cultivation of Neisseria gonorrhoeae

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N. gonorrrhoeae was grown overnight at 37°C and 5% CO2 on agar plates containing gonococcal base agar (10 g/l Bacto agar [BD Biosciences, Bedford, MA, USA], 5 g/l NaCl [Roth, Darmstadt, Germany], 4 g/l K2HPO4 [Roth], 1 g/l KH2PO4 [Roth], 15 g/l Bacto Proteose Peptone No. 3 [BD], 0.5 g/l soluble starch [Sigma–Aldrich, St. Louis, MO, USA]), and the following supplements: 1 g/l D-Glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL × H20 (Roth), 1 mg/l thiamine pyrophosphate (Sigma–Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma–Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma–Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma–Aldrich). Before each experiment, gonococcal colonies were resuspended in GC-medium.
Oldewurtel E.R., Kouzel N., Dewenter L., Henseler K, & Maier B. (2015). Differential interaction forces govern bacterial sorting in early biofilms. eLife, 4, e10811.
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