Skin sections from IMQ-treated skin were embedded and frozen in OCT and stained with H&E and Gr-1 mAb for IHC. Epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked and then stained with purified rat-anti-mouse Gr-1 Ab (1:50 dilution) following with goat-anti-rat IgG secondary antibody (1:200 dilution, Southern Biotech). Slides were developed with AEC substrate solution (Vector Laboratories) and then counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Aec substrate solution
Manufactured by Vector Laboratories
AEC substrate solution is a reagent used in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) to visualize the presence of target proteins or antigens. The solution contains 3-amino-9-ethylcarbazole, which is an enzymatic substrate that produces a reddish-brown color upon reaction with the enzyme label, typically horseradish peroxidase. This substrate solution is used to develop and detect the signal in these immunoassay techniques.
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Lab products found in correlation
3 protocols using aec substrate solution
1
Histological Analysis of Imiquimod-Induced Skin Inflammation
2
Histological Analysis of Epidermal Thickness
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Skin sections were stained with H&E. Epidermal thickness was determined by measuring the mean interfollicular distance under the microscope. For IHC staining, skin cryosections were fixed, blocked, and stained with purified rabbit-anti-mouse Ki-67 Ab (1:200 dilution), followed by anti-rabbit IgG secondary antibody (1:200 dilution). Slides were developed with AEC substrate solution (Vector Laboratories) and then counterstained with hematoxylin. Images were acquired at 200× magnification using a Keyence BZ-X800 microscope.
3
Histological Analysis of Imiquimod-Induced Skin Inflammation
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