All solutions were prepared using double de-ionized (DDI) water (18 MΩ cm−1, produced by the Nanopure treatment system purchased from Sybron Barnstead, Boston, MA, USA). Labware was acid washed with 10% nitric acid (Fisher Scientific, Fair Lawn, NJ, USA) to remove metals. Cells were washed with phosphate-buffered saline (PBS) without added calcium and magnesium (Mediatech, Inc. A Corning Subsidiary, Manassas, VA, USA). Lysis buffer components included sodium chloride (Fisher Scientific, Fair Lawn, NJ, USA), Triton x-100 (Sigma-Aldrich, St. Louis, MO, USA), tris(hydroxymethyl)aminomethane (Tris) (Acros Organics, Morris Plains, NJ, USA), and Mini EDTA-free Protease Inhibitor Cocktail Tablets (Roche Life Science, Indianapolis, IN, USA). The mobile phase for SEC contained 50 mM ammonium acetate (Fisher Scientific, Fair Lawn, NJ, USA) and 0.5% methanol (Fisher Scientific, Fair Lawn, NJ, USA) in DDI water, adjusted to pH 7.4 using ammonium hydroxide (Fisher Scientific, Fair Lawn, NJ, USA). The Gel Filtration Standard (Bio-Rad Laboratories, Inc., Hercules, CA, USA) used for SEC was a lyophilized mixture of molecular weight markers at 1.35, 17, 44, 158, and 670 kDa which was dissolved in DDI water. Total protein concentration was determined using a Qubit® Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
Tris hydroxymethyl aminomethane tris
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
Tris(hydroxymethyl)aminomethane (Tris) is a widely used organic compound that serves as a buffer in various biochemical and analytical applications. It is a white, crystalline solid that is highly soluble in water. Tris is primarily used to maintain a stable pH environment in solutions, enabling efficient biomolecular reactions and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Thermo Fisher Scientific (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Imidazole, by Thermo Fisher Scientific (3 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Thermo Fisher Scientific (3 mentions)
Dl dithiothroitol dtt, by GoldBio (3 mentions)
Ampicillin, by GoldBio (2 mentions)
Dehydrated luria bertani broth, by Thermo Fisher Scientific (2 mentions)
Isopropyl β d thiogalactopyranoside iptg, by GoldBio (2 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (2 mentions)
Z rlrgg 7 amino 4 methyl courmarin peptide amc, by Bachem (2 mentions)
Ubiquitin 7amino 4 methylcourmarin ub amc, by R&D Systems (2 mentions)
Human isg15 7amino 4 methylcourmarin isg15 amc, by R&D Systems (2 mentions)
Triton x 100, by Merck Group (1 mentions)
Qubit protein assay kit, by Thermo Fisher Scientific (1 mentions)
Gel filtration standard, by Bio-Rad (1 mentions)
Mini edta free protease inhibitor cocktail tablet, by Roche (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Nitric acid, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
9 protocols using tris hydroxymethyl aminomethane tris
1
Protein Purification and Characterization
2
Luminescent Silica Nanoparticles for Antibiotic Detection
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N-(b-aminoethyl)-g-aminopropylmethyl dimethoxysilane (AEAPMS), europium nitrate (Eu(NO3)3·6H2O, 99.99%), tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTES), citric acid anhydrous(cit), and hexadecyl trimethyl ammonium bromide (CTAB) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). N, N-dimethylformamide (DMF), hydrochloric acid (HCl), and ethanol were purchased from Kermel analytical reagent Co., Ltd. (Tianjing, China). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimidehy -drochloride (EDC), N-hydroxysuccinimide (NHS), Dicyclohexylcarbodiimide (DCC), and tris(hydroxymethyl)aminomethane (Tris) were purchased from Acros Organics. Tetracycline (TC) was purchased from Xiya reagent Co., Ltd. (Shandong, China). Oxytetracycline (OTC), thiamphenicol, kanamycin sulfate, ofloxacin, chloramphenicol, and potassium bromide (KBr, spectral pure) were purchased from Adamas-beta Reagent Co., Ltd. (Shanghai, China). Potassium chloride (KCl), sodium chloride (NaCl), calcium chloride (CaCl2), magnesium chloride (MgCl2), ferric chloride hexahydrate (FeCl3·6H2O), sodium nitrate (NaNO3), potassium nitrate (KNO3), potassium sulfate (K2SO4), sodium sulfate (Na2SO4), and sodium hydroxide (NaOH) were purchased from Shanghai Titan Scientific Co., Ltd. (Shanghai, China). All reagents are of analytical grade or higher and used without further treatment.
3
Quantification of Glutathione Using HPLC
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HPLC-grade acetonitrile, HPLC-grade water and tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific (Loughborough, UK); Ac-Tyr-OEt monohydrate (purity, HPLC 99.3 %, water content, Karl Fischer titration 5.94 %) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany); dimethylsulfoxide (DMSO), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), formic acid, reduced l -glutathione (GSH; purity, HPLC 99.3 %, purity, iodine titration 99 %) and iodoacetamide were purchased from Sigma-Aldrich (Dorset, UK); hydrochloric acid was purchased from VWR International (Leicestershire, UK); polystyrene 96-well plates were purchased from Greiner Bio One (Gloucester, UK); iT3 (ALVLIAFAQYLQQCPFEDHVK, in which valine is fully labelled with 13C and 15N; HPLC-UV purity > 95 %) was purchased from a commercial source (details available from the authors on request).
4
Synthesis of Inorganic Nanoparticles
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Dopamine hydrochloride (98%), iron(III) chloride hexahydrate (>99%), iron(II) chloride tetrahydrate (>99%) sodium chloride (>99%) and citric acid (>99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Sodium bicarbonate (>99%), sodium carbonate (>99%), tris(hydroxymethyl) aminomethane (Tris), sodium phosphate dibasic (99.2%) and magnesium chloride hexahydrate (>99%) were purchased from Fisher Scientific (Hampton, NH, USA) and used as received. Calcium chloride dihydrate (>99%) was purchased from BDH VWR International (Radnor, PA, USA). Water was purified (18.2 MΩ·cm) using Direct-Q 3 UV-R system (EMD Millipore, Billerica, MA, USA). Silicon wafers with native oxide were purchased from Silicon Quest International (San Jose, CA, USA; 1” diameter, phosphorus doped). Indium tin oxide (ITO) pieces were purchased from University Wafer (Boston, MA, USA; 20 Ω/sq; ITO on glass).
5
Protein Expression and Purification
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Poly-ethylene glycol (PEG) 3350 was purchased from Sigma Life Sciences, tri-ammonium citrate was purchased from Sigma Life Sciences, Ampicillin was purchased from GoldBio, dehydrated Luria-Bertani (LB) Broth was purchased from Fisher Scientific, DL-dithiothroitol (DTT) and isopropyl-β-d-thiogalactopyranoside (IPTG) were purchased from GoldBio. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from Fisher BioReagents. Imidazole was purchased from Acros Organics; tris(hydroxymethyl)aminomethane (Tris) was purchased from Fisher Scientific. Sodium chloride (NaCl) was purchased from Fisher Chemical, and bovine serum albumin (BSA) was purchased from Sigma Life Science.
6
Radiolabeling Bovine Serum Albumin
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Bovine serum albumins (laboratory grade albumin, albumin tissue culture grade and low endotoxin, essentially IgG free albumin) were purchased from Sigma (Dorset, UK). S-2-(4-Isothiocyanatobenzyl)-diethylenetriamine pentaacetic acid (p-SCN-Bn-DTPA) was purchased from Macrocyclics (Dallas, USA). 111InCl3 was purchased from Mallinckrodt Medical Inc. (Petten, Netherlands). Phosphate buffered saline (PBS) tablets were purchased from Oxoid (Basingstoke, UK) and prepared using ultrapure water according to the manufacturer's instructions. Foetal bovine serum was purchased from Life Technologies (USA). Diethylenetriamine pentaacetic acid (DTPA) and ammonium acetate were purchased from Fluka (UK). Sodium hydroxide (NaOH) and Tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific (Loughborough, UK). Glutaraldehyde (50% vol/vol in water) was purchased from Aldrich (UK). Ethanol was purchased from VWR (Radnor, USA).
7
Ubiquitin and ISG15 Substrate Assay
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Z-RLRGG-7-amino-4-methyl-courmarin (peptide-AMC) was purchased from Bachem. Ubiquitin-7amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15-7amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem; DLdithiothroitol (DTT) was purchased from GoldBio, and isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from GoldBio. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from Fisher BioReagents. Imidazole was purchased from Acros Organics; tris(hydroxymethyl)aminomethane (Tris) was purchased from Fisher Scientific. Losartan and Sodium chloride (NaCl) were purchased from Fisher Chemical and bovine serum albumin (BSA) was purchased from Sigma Life Science.
8
Fabrication of Polysulfone-Cellulose Acetate Membranes
9
Recombinant Protein Expression and Purification
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