DPPH assay kit (Solarbio, China) was used to investigate the ability of Pt NPs and Pt NWs to scavenge free radicals. According to instructions, each sample with a different concentration formed a well-mixed solution for 30 min in the dark at room temperature. The absorbance wavelength of each sample was recorded at 515 nm by a UV–visible spectrophotometer. Finally, the results of the free radical scavenging ability were calculated according to the scavenging ratio (%).
Dpph assay kit
Manufactured by Solarbio
Sourced in China
The DPPH Assay Kit is a laboratory tool used to measure the antioxidant capacity of a sample. It contains the DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, which changes color when reduced by antioxidants. The kit provides a method to quantify the antioxidant content in a variety of samples.
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Lab products found in correlation
3 protocols using dpph assay kit
1
Pt NPs and Pt NWs DPPH Radical Scavenging
2
DPPH Assay for Antioxidant Capacity
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The free radical scavenging capacity of BBIQ compounds was assayed using the DPPH Assay Kit (Solarbio, Beijing, China, BC4755). Briefly, testing compounds were diluted in the provided solution to the concentration of 1, 5, 10, or 50 µM. An aliquot of testing compound solution (10 µl) was mixed thoroughly with 190 µl of DPPH working solution in 96-well plate and incubated at RT in dark for 30 min. After reaction, the absorbance (A) of the reaction mixture at the wave length of 515 nm was assayed using an automatic microplate reader (Thermo, Epoch 2). The DPPH scavenging rate is calculated by the formula: positive control: DVC% = [(A blank − A positive control)/A blank] × 100%; sample: D% = [[A blank − (A sample − A control)]/A blank] × 100%.
3
DPPH Radical Scavenging Assay
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The free radical scavenging capacity of a testing compound was assayed using the DPPH Assay Kit (Solarbio, BC4755). Briefly, NAC was diluted in the provided solution to the final concentration of 5 mM and all other testing compounds were diluted to 100 µM. An aliquot of testing compound solution (5 µL) was mixed thoroughly with 195 µL of DPPH working solution in 96-well plate and incubated in dark for 30 min at RT. After reaction, the absorbance (A) of the reaction mixture was assayed at the wavelength of 515 nm using an automatic microplate reader (Thermo, Epoch 2). The DPPH scavenging rate is calculated as: DPPH Scavenging Activity = [[A blank -(A sample -A control)]/A blank] × 100%.
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