Accuzol total rna extraction reagent

Manufactured by Bioneer

Sourced in Cameroon

AccuZol Total RNA Extraction Reagent is a reagent used for the extraction and purification of total RNA from various biological samples. It is a complete solution for RNA isolation, providing a simple and efficient method to obtain high-quality RNA.

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Dnase 1, by Thermo Fisher Scientific (2 mentions) Mmessage mmachine sp6 kit, by Thermo Fisher Scientific (1 mentions) Accupower rocketscript rt premix, by Bioneer (1 mentions) Topscript cdna synthesis kit, by Enzynomics (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Accupower cyclescript rt premix, by Bioneer (1 mentions)

Close spelling variants of this product

Accuzol (20 citations) AccuZol™ reagent (9 citations)

6 protocols using accuzol total rna extraction reagent

Zebrafish psmd8 mRNA Rescue Protocol

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The full-length zebrafish psmd8 was amplified by RT-PCR using zebrafish cDNA, which was synthesized with AccuPower® Rocket Script RT Premix (K-2101, Bioneer, Korea) from total RNA, extracted from zebrafish embryos at 24hpf using the Accuzol™ Total RNA Extraction Reagent (K-3090, Bioneer, Korea). The zebrafish psmd8 cDNA was subcloned into pCS2+ (Clontech) and the Capped mRNA was synthesized using the mMESSAGE mMACHINE® SP6 Kit (AM1340, Thermo Fisher Scientific). The zebrafish psmd8 mRNA (200 pg/nl) was injected into the psmd8 MO-injected embryos. The following primer sequences were used for the RT-PCR:

Forward primer, 5’-CCGGAATTCATGGGCGTCTTGTTGAAAGAG-3’,

Reverse primer, 5’-GCGCGTCTAGACTACACAATCATTTCCAGCTGTCTTGC-3’.

Kim J.H., Ki S.M., Joung J.G., Scott E., Heynen-Genel S., Aza-Blanc P., Kwon C.H., Kim J., Gleeson J.G, & Lee J.E. (2016). Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation. Biochimica et biophysica acta, 1863(6 Pt A), 1307-1318.

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RAW 264.7 Macrophage Inflammatory Response

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RAW 264.7 macrophages were seeded onto 12-well plates (2 × 10⁵ cells/well) and incubated overnight. The cells were then pre-treated with MBJ (20, 50, 100, and 150 μg/mL) for 2 h and stimulated with LPS (1 μg/mL) for 3 h. Total RNA was extracted using the Accuzol total RNA extraction reagent (Bioneer Corporation, Daejeon, Korea). The total RNA (1 μg) was then reverse transcribed into complementary DNA (cDNA) using a TOPscript cDNA synthesis kit (Enzynomics, Daejeon, Korea). The amplification conditions were the following: 95 °C (5 min) initial denaturation followed by 25 cycles of 95 °C for 5 s and annealing/extension at 55 °C (30 s). The gene expression levels were normalized relative to those of the reference gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH).

Lee S., Ha J., Park J., Kang E., Jeon S.H., Han S.B., Ningsih S., Paik J.H, & Cho S. (2021). Antioxidant and Anti-Inflammatory Effects of Bischofia javanica (Blume) Leaf Methanol Extracts through the Regulation of Nrf2 and TAK1. Antioxidants, 10(8), 1295.

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Quantifying mRNA Expression Levels

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After treatment for 36 h with 5 mM Met or NH₄Cl, total RNA was extracted using AccuZol Total RNA Extraction Reagent (Bioneer, Daejeon, Korea) on the basis of the manufacturer’s instructions [19 (link)]. Then, DNaseI (Thermo Scientific, Waltham, MA, USA) was used to eliminate genomic DNA, and a Nano Drop 2000 (Thermo Scientific, Waltham, MA, USA) was used to evaluate the RNA quality and quantity. Then, a Prime Script RT Reagent Kit (Takara, Dalian, China) was applied to reverse-transcribe the RNA into cDNA(complementary Deoxyribonucleic acid). The 2^−ΔΔCT method was used to determine the relative mRNA expression levels [20 (link)]. The details of the primers used for the various genes are shown in Table 1.

Kong Z., Zhou C., Kang J, & Tan Z. (2020). Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats. Animals : an Open Access Journal from MDPI, 10(11), 2079.

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Quantitative RT-PCR Gene Expression Analysis

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Total RNA was extracted using AccuZol Total RNA Extraction Reagent (Bioneer, Korea) in accordance with the manufacturer’s protocol. The PCR primers were purchased from Bioneer (Table 1). The PCR conditions were as follows: 95°C for 10 min, followed by 40 cycles at 95°C for 15 s, 60°C for 15 s, and 72°C for 20 s. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the internal control gene. Normalized expression change was expressed as 2^–ΔΔCt (The control GAPDH was set to 1).

Choo S., An M, & Lim Y.H. (2023). Protective Effects of Heat-Killed Ruminococcus albus against β-Amyloid-Induced Apoptosis on SH-SY5Y Cells. Journal of Microbiology and Biotechnology, 34(1), 85-93.

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Total RNA Extraction and cDNA Synthesis

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The total RNA was isolated using AccuZol Total RNA Extraction Reagent (Bioneer Corporation, Daejeon, South Korea) according to the manufacturer’s instructions. cDNA was synthesized using the AccuPower CycleScript RT Premix (dT₂₀; Bioneer), and the synthesis was carried out as follows: 40 cycles for 1 minute at 15°C, 4 minutes at 50°C, 1 cycle for 5 minutes at 95°C, 60 minutes at 50°C, and 5 minutes at 95°C.

Cho T.J., Lee D.H., Choi B.H., Shinn H.K, & Park C.S. (2021). Hypoxia-Induced Suppression of Antiapoptotic Bcl-2 Expression in Human Bladder Tumor Cells Is Regulated by Caveolin-1-Dependent Adenosine Monophosphate-Activated Protein Kinase Activity. International Neurourology Journal, 25(2), 137-149.

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Age-Dependent Lung Tissue Analysis

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Eighteen male Wistar rats were obtained from the Animal House of the Faculty of Veterinary Medicine, University of Qadisiyah, and were divided into three groups according to age. The healthy rats were chosen for this study to avoid any physiological or biological changes in the organs and tissue of rats. The first group was 2 weeks, the second group was 2 months, and the third group was 6-8 months.
Rats were anesthetized before being killed by chloroform through the artificial inspiration for 2 min. Animals were killed, and samples of lung tissue were collected for each group. The samples were divided into two group samples, the first group samples were stored in 10% neutral buffered formalin (NBF) for histology study and second group samples were stored in AccuZol™ Total RNA Extraction Reagent (Bioneer, Korea) for real-time quantitative polymerase chain reaction (RT-qPCR).

Almhanna H., Al-Mamoori N.A, & Naser H.H. (2022). mRNA expression of the severe acute respiratory syndrome-coronavirus 2 angiotensin-converting enzyme 2 receptor in the lung tissue of Wistar rats according to age. Veterinary World, 15(2), 427-434.

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