Cellular ATP levels were detected with an ATPliteTM Luminescence Assay kit (PerkinElmer) as per the manufacturer's instructions. In general, TM3 cells were pretreated with DEHP at the indicated concentrations for 24 h. ATP inhibitor cocktail was added to inhibit ATP consumption. Then 50 µL of lysis buffer was added, followed by addition of substrate. Luminescence was measured in a luminescence counter (PerkinElmer). NAD+ assay was performed by using a commercial NAD/NADH assay kit (#ab65348).
Atplitetm luminescence assay kit
Manufactured by PerkinElmer
The ATPliteTM Luminescence Assay kit is a biochemical assay used to detect and quantify the presence of adenosine triphosphate (ATP) in samples. The kit utilizes a luciferase-based reaction to generate a luminescent signal proportional to the ATP concentration in the sample. The assay provides a sensitive and reliable method for measuring ATP levels in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using atplitetm luminescence assay kit
1
Cellular ATP and NAD+ Quantification
2
ATP Quantification in Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1*106 cultured cells were harvested in 200 μl PBS and cell lysate was achieved by sonication (in ice-water bath) before the homogenate was centrifugated at 12,000 g for 10 min. Finally, in 96-well plates, 10 μl of the supernatant was mixed well with 100 μl of luciferase reagent, which catalyzed the light production from ATP and luciferin ATPliteTM Luminescence Assay kit (PerkinElmer) according the manufacturer's instructions. Luminescence was measured by luminometer (Fluroskan Ascent FL, Thermo). Standard curve was also generated and the amount of ATP in the experiments samples was calculated. Total ATP levels were expressed as nmol/106 cell.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!